Study on titanium dioxide nanoparticles as MALDI MS matrix for the determination of lipids in the brain

The structures of lipids are diverse, and thus, lipids show various biological functions. Systematic determination of lipids in organisms has always been a concern. In this paper, a methodology on the matrix-assisted laser desorption ionization mass spectrometry (MALDI MS), with titanium dioxide nan...

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Autores principales:	Peng Congnan, Zhang Qian, Liu Jian-an, Wang Zhen-peng, Zhao Zhen-wen, Kang Ning, Chen Yuxin, Huo Qing
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	tio2 nps maldi mass spectrometry lipid Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/34887cfcde1e4901ba8d4fb8321b1d50
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spelling	oai:doaj.org-article:34887cfcde1e4901ba8d4fb8321b1d502021-12-05T14:10:50ZStudy on titanium dioxide nanoparticles as MALDI MS matrix for the determination of lipids in the brain2191-955010.1515/gps-2021-0056https://doaj.org/article/34887cfcde1e4901ba8d4fb8321b1d502021-11-01T00:00:00Zhttps://doi.org/10.1515/gps-2021-0056https://doaj.org/toc/2191-9550The structures of lipids are diverse, and thus, lipids show various biological functions. Systematic determination of lipids in organisms has always been a concern. In this paper, a methodology on the matrix-assisted laser desorption ionization mass spectrometry (MALDI MS), with titanium dioxide nanoparticles (TiO2 NPs) as the matrix, was studied for lipid determination. The results showed that the following conditions were preferable in the determination of small-molecule lipids (such as hypoxanthine, guanosine, uridine, and cytidine), lipid standards (such as GC, GM, TG, phosphatidylethanolamine, phosphatidylcholine, and ceramide), and mixed lipids (extracted from brain homogenate with methanol alone and with the B&D method): TiO2 NPs as the matrix, absolute ethanol as the solvent, 1 mg of TiO2 NPs dispersed in 1 mL of absolute ethanol as the matrix solution, NaCl as the ionization reagent, and positive mass spectrometry (MS) as the mode. Modified TiO2 NP as a new matrix for MALDI MS will be a future research direction; in addition, the characteristics of TiO2 NPs make it a potential matrix for imaging MS.Peng CongnanZhang QianLiu Jian-anWang Zhen-pengZhao Zhen-wenKang NingChen YuxinHuo QingDe Gruyterarticletio2 npsmaldi mass spectrometrylipidChemistryQD1-999ENGreen Processing and Synthesis, Vol 10, Iss 1, Pp 700-710 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	tio2 nps maldi mass spectrometry lipid Chemistry QD1-999
spellingShingle	tio2 nps maldi mass spectrometry lipid Chemistry QD1-999 Peng Congnan Zhang Qian Liu Jian-an Wang Zhen-peng Zhao Zhen-wen Kang Ning Chen Yuxin Huo Qing Study on titanium dioxide nanoparticles as MALDI MS matrix for the determination of lipids in the brain
description	The structures of lipids are diverse, and thus, lipids show various biological functions. Systematic determination of lipids in organisms has always been a concern. In this paper, a methodology on the matrix-assisted laser desorption ionization mass spectrometry (MALDI MS), with titanium dioxide nanoparticles (TiO2 NPs) as the matrix, was studied for lipid determination. The results showed that the following conditions were preferable in the determination of small-molecule lipids (such as hypoxanthine, guanosine, uridine, and cytidine), lipid standards (such as GC, GM, TG, phosphatidylethanolamine, phosphatidylcholine, and ceramide), and mixed lipids (extracted from brain homogenate with methanol alone and with the B&D method): TiO2 NPs as the matrix, absolute ethanol as the solvent, 1 mg of TiO2 NPs dispersed in 1 mL of absolute ethanol as the matrix solution, NaCl as the ionization reagent, and positive mass spectrometry (MS) as the mode. Modified TiO2 NP as a new matrix for MALDI MS will be a future research direction; in addition, the characteristics of TiO2 NPs make it a potential matrix for imaging MS.
format	article
author	Peng Congnan Zhang Qian Liu Jian-an Wang Zhen-peng Zhao Zhen-wen Kang Ning Chen Yuxin Huo Qing
author_facet	Peng Congnan Zhang Qian Liu Jian-an Wang Zhen-peng Zhao Zhen-wen Kang Ning Chen Yuxin Huo Qing
author_sort	Peng Congnan
title	Study on titanium dioxide nanoparticles as MALDI MS matrix for the determination of lipids in the brain
title_short	Study on titanium dioxide nanoparticles as MALDI MS matrix for the determination of lipids in the brain
title_full	Study on titanium dioxide nanoparticles as MALDI MS matrix for the determination of lipids in the brain
title_fullStr	Study on titanium dioxide nanoparticles as MALDI MS matrix for the determination of lipids in the brain
title_full_unstemmed	Study on titanium dioxide nanoparticles as MALDI MS matrix for the determination of lipids in the brain
title_sort	study on titanium dioxide nanoparticles as maldi ms matrix for the determination of lipids in the brain
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/34887cfcde1e4901ba8d4fb8321b1d50
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Study on titanium dioxide nanoparticles as MALDI MS matrix for the determination of lipids in the brain

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