Design And Characterisation Of Novel Sorafenib-Loaded Carbon Nanotubes With Distinct Tumour-Suppressive Activity In Hepatocellular Carcinoma

Mahmoud MA Elsayed,1 Mahmoud E Mostafa,2 Eman Alaaeldin,2,3 Hatem AA Sarhan,2 Montaser ShA Shaykoon,4 Shady Allam,5 Ahmed RH Ahmed,6 Bakheet EM Elsadek7 1Department of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag University, Sohag, Egypt; 2Department of Pharmaceutics, Faculty of P...

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Autores principales: Elsayed MMA, Mostafa ME, Alaaeldin E, Sarhan HAA, Shaykoon MS, Allam S, Ahmed ARH, Elsadek BEM
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Publicado: Dove Medical Press 2019
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spelling oai:doaj.org-article:3489a32513b34e969844f918bb4d15a32021-12-02T06:41:24ZDesign And Characterisation Of Novel Sorafenib-Loaded Carbon Nanotubes With Distinct Tumour-Suppressive Activity In Hepatocellular Carcinoma1178-2013https://doaj.org/article/3489a32513b34e969844f918bb4d15a32019-10-01T00:00:00Zhttps://www.dovepress.com/design-and-characterisation-of-novel-sorafenib-loaded-carbon-nanotubes-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mahmoud MA Elsayed,1 Mahmoud E Mostafa,2 Eman Alaaeldin,2,3 Hatem AA Sarhan,2 Montaser ShA Shaykoon,4 Shady Allam,5 Ahmed RH Ahmed,6 Bakheet EM Elsadek7 1Department of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag University, Sohag, Egypt; 2Department of Pharmaceutics, Faculty of Pharmacy, Minia University, Minia, Egypt; 3Department of Clinical Pharmacy, Deraya University, Minia, Egypt; 4Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut, Egypt; 5Department of Pharmacology and Toxicology, Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh, Egypt; 6Department of Pathology, Faculty of Medicine, Sohag University, Sohag, Egypt; 7Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut, EgyptCorrespondence: Bakheet EM ElsadekBiochemistry and Molecular Biology, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, P. O. Box 71524, Assiut, EgyptTel +201110596270Email bakheet.elkot@azhar.edu.egPurpose: Over the past 30 years, no consistent survival benefits have been recorded for anticancer agents of advanced hepatocellular carcinoma (HCC), except for the multikinase inhibitor sorafenib (Nexavar®), which clinically achieves only ∼3 months overall survival benefit. This modest benefit is attributed to limited aqueous solubility, slow dissolution rate and, consequently, limited absorption from the gastrointestinal tract. Thus, novel formulation modalities are in demand to improve the bioavailability of the drug to attack HCC in a more efficient manner. In the current study, we aimed to design a novel sorafenib-loaded carbon nanotubes (CNTs) formula that is able to improve the therapeutic efficacy of carried cargo against HCC and subsequently investigate the antitumour activity of this formula.Materials and methods: Sorafenib was loaded on functionalized CNTs through physical adsorption, and an alginate-based method was subsequently applied to microcapsulate the drug-loaded CNTs (CNTs-SFN). The therapeutic efficacy of the new formula was estimated and compared to that of conventional sorafenib, both in vitro (against HepG2 cells) and in vivo (in a DENA-induced HCC rat model).Results: The in vitro MTT anti-proliferative assay revealed that the drug-loaded CNTs formula was at least two-fold more cytotoxic towards HepG2 cells than was sorafenib itself. Moreover, the in vivo animal experiments proved that our innovative formula was superior to conventional sorafenib at all assessed end points. Circulating AFP-L3% was significantly decreased in the CNTs-SFN-MCs-treated group (14.0%) in comparison to that of the DENA (40.3%) and sorafenib (38.8%) groups. This superiority was further confirmed by Western blot analysis and immunofluorescence assessment of some HCC-relevant biomarkers.Conclusion: Our results firmly suggest the distinctive cancer-suppressive nature of CNTs-SFN-MCs, both against HepG2 cells in vitro and in a DENA-induced HCC rat model in vivo, with a preferential superiority over conventional sorafenib.Keywords: carbon nanotubes, sorafenib, DENA, hepatocellular carcinoma, microcapsulesElsayed MMAMostafa MEAlaaeldin ESarhan HAAShaykoon MSAllam SAhmed ARHElsadek BEMDove Medical Pressarticlecarbon nanotubessorafenibdenahepatocellular carcinomamicrocapsulesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 8445-8467 (2019)
institution DOAJ
collection DOAJ
language EN
topic carbon nanotubes
sorafenib
dena
hepatocellular carcinoma
microcapsules
Medicine (General)
R5-920
spellingShingle carbon nanotubes
sorafenib
dena
hepatocellular carcinoma
microcapsules
Medicine (General)
R5-920
Elsayed MMA
Mostafa ME
Alaaeldin E
Sarhan HAA
Shaykoon MS
Allam S
Ahmed ARH
Elsadek BEM
Design And Characterisation Of Novel Sorafenib-Loaded Carbon Nanotubes With Distinct Tumour-Suppressive Activity In Hepatocellular Carcinoma
description Mahmoud MA Elsayed,1 Mahmoud E Mostafa,2 Eman Alaaeldin,2,3 Hatem AA Sarhan,2 Montaser ShA Shaykoon,4 Shady Allam,5 Ahmed RH Ahmed,6 Bakheet EM Elsadek7 1Department of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag University, Sohag, Egypt; 2Department of Pharmaceutics, Faculty of Pharmacy, Minia University, Minia, Egypt; 3Department of Clinical Pharmacy, Deraya University, Minia, Egypt; 4Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut, Egypt; 5Department of Pharmacology and Toxicology, Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh, Egypt; 6Department of Pathology, Faculty of Medicine, Sohag University, Sohag, Egypt; 7Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut, EgyptCorrespondence: Bakheet EM ElsadekBiochemistry and Molecular Biology, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, P. O. Box 71524, Assiut, EgyptTel +201110596270Email bakheet.elkot@azhar.edu.egPurpose: Over the past 30 years, no consistent survival benefits have been recorded for anticancer agents of advanced hepatocellular carcinoma (HCC), except for the multikinase inhibitor sorafenib (Nexavar®), which clinically achieves only ∼3 months overall survival benefit. This modest benefit is attributed to limited aqueous solubility, slow dissolution rate and, consequently, limited absorption from the gastrointestinal tract. Thus, novel formulation modalities are in demand to improve the bioavailability of the drug to attack HCC in a more efficient manner. In the current study, we aimed to design a novel sorafenib-loaded carbon nanotubes (CNTs) formula that is able to improve the therapeutic efficacy of carried cargo against HCC and subsequently investigate the antitumour activity of this formula.Materials and methods: Sorafenib was loaded on functionalized CNTs through physical adsorption, and an alginate-based method was subsequently applied to microcapsulate the drug-loaded CNTs (CNTs-SFN). The therapeutic efficacy of the new formula was estimated and compared to that of conventional sorafenib, both in vitro (against HepG2 cells) and in vivo (in a DENA-induced HCC rat model).Results: The in vitro MTT anti-proliferative assay revealed that the drug-loaded CNTs formula was at least two-fold more cytotoxic towards HepG2 cells than was sorafenib itself. Moreover, the in vivo animal experiments proved that our innovative formula was superior to conventional sorafenib at all assessed end points. Circulating AFP-L3% was significantly decreased in the CNTs-SFN-MCs-treated group (14.0%) in comparison to that of the DENA (40.3%) and sorafenib (38.8%) groups. This superiority was further confirmed by Western blot analysis and immunofluorescence assessment of some HCC-relevant biomarkers.Conclusion: Our results firmly suggest the distinctive cancer-suppressive nature of CNTs-SFN-MCs, both against HepG2 cells in vitro and in a DENA-induced HCC rat model in vivo, with a preferential superiority over conventional sorafenib.Keywords: carbon nanotubes, sorafenib, DENA, hepatocellular carcinoma, microcapsules
format article
author Elsayed MMA
Mostafa ME
Alaaeldin E
Sarhan HAA
Shaykoon MS
Allam S
Ahmed ARH
Elsadek BEM
author_facet Elsayed MMA
Mostafa ME
Alaaeldin E
Sarhan HAA
Shaykoon MS
Allam S
Ahmed ARH
Elsadek BEM
author_sort Elsayed MMA
title Design And Characterisation Of Novel Sorafenib-Loaded Carbon Nanotubes With Distinct Tumour-Suppressive Activity In Hepatocellular Carcinoma
title_short Design And Characterisation Of Novel Sorafenib-Loaded Carbon Nanotubes With Distinct Tumour-Suppressive Activity In Hepatocellular Carcinoma
title_full Design And Characterisation Of Novel Sorafenib-Loaded Carbon Nanotubes With Distinct Tumour-Suppressive Activity In Hepatocellular Carcinoma
title_fullStr Design And Characterisation Of Novel Sorafenib-Loaded Carbon Nanotubes With Distinct Tumour-Suppressive Activity In Hepatocellular Carcinoma
title_full_unstemmed Design And Characterisation Of Novel Sorafenib-Loaded Carbon Nanotubes With Distinct Tumour-Suppressive Activity In Hepatocellular Carcinoma
title_sort design and characterisation of novel sorafenib-loaded carbon nanotubes with distinct tumour-suppressive activity in hepatocellular carcinoma
publisher Dove Medical Press
publishDate 2019
url https://doaj.org/article/3489a32513b34e969844f918bb4d15a3
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