The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection

The Pseudomonas aeruginosa lytic polysaccharide monooxygenase CbpD, prevalent in clinical isolates, has been proposed to act as a virulence factor. Here, the authors combine structural work, in silico simulations, enzymatic activity and in vitro and in vivo experiments to further delineate the role...

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Autores principales:	Fatemeh Askarian, Satoshi Uchiyama, Helen Masson, Henrik Vinther Sørensen, Ole Golten, Anne Cathrine Bunæs, Sophanit Mekasha, Åsmund Kjendseth Røhr, Eirik Kommedal, Judith Anita Ludviksen, Magnus Ø. Arntzen, Benjamin Schmidt, Raymond H. Zurich, Nina M. van Sorge, Vincent G. H. Eijsink, Ute Krengel, Tom Eirik Mollnes, Nathan E. Lewis, Victor Nizet, Gustav Vaaje-Kolstad
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/3489cf0fa4cd4b18b05a1a1e7e7583d6
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spelling	oai:doaj.org-article:3489cf0fa4cd4b18b05a1a1e7e7583d62021-12-02T14:29:04ZThe lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection10.1038/s41467-021-21473-02041-1723https://doaj.org/article/3489cf0fa4cd4b18b05a1a1e7e7583d62021-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21473-0https://doaj.org/toc/2041-1723The Pseudomonas aeruginosa lytic polysaccharide monooxygenase CbpD, prevalent in clinical isolates, has been proposed to act as a virulence factor. Here, the authors combine structural work, in silico simulations, enzymatic activity and in vitro and in vivo experiments to further delineate the role of CbpD and show that its deletion renders P. aeruginosa unable to establish a lethal systemic infection, leading to enhanced bacterial clearance in a mouse model of infection.Fatemeh AskarianSatoshi UchiyamaHelen MassonHenrik Vinther SørensenOle GoltenAnne Cathrine BunæsSophanit MekashaÅsmund Kjendseth RøhrEirik KommedalJudith Anita LudviksenMagnus Ø. ArntzenBenjamin SchmidtRaymond H. ZurichNina M. van SorgeVincent G. H. EijsinkUte KrengelTom Eirik MollnesNathan E. LewisVictor NizetGustav Vaaje-KolstadNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Fatemeh Askarian Satoshi Uchiyama Helen Masson Henrik Vinther Sørensen Ole Golten Anne Cathrine Bunæs Sophanit Mekasha Åsmund Kjendseth Røhr Eirik Kommedal Judith Anita Ludviksen Magnus Ø. Arntzen Benjamin Schmidt Raymond H. Zurich Nina M. van Sorge Vincent G. H. Eijsink Ute Krengel Tom Eirik Mollnes Nathan E. Lewis Victor Nizet Gustav Vaaje-Kolstad The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection
description	The Pseudomonas aeruginosa lytic polysaccharide monooxygenase CbpD, prevalent in clinical isolates, has been proposed to act as a virulence factor. Here, the authors combine structural work, in silico simulations, enzymatic activity and in vitro and in vivo experiments to further delineate the role of CbpD and show that its deletion renders P. aeruginosa unable to establish a lethal systemic infection, leading to enhanced bacterial clearance in a mouse model of infection.
format	article
author	Fatemeh Askarian Satoshi Uchiyama Helen Masson Henrik Vinther Sørensen Ole Golten Anne Cathrine Bunæs Sophanit Mekasha Åsmund Kjendseth Røhr Eirik Kommedal Judith Anita Ludviksen Magnus Ø. Arntzen Benjamin Schmidt Raymond H. Zurich Nina M. van Sorge Vincent G. H. Eijsink Ute Krengel Tom Eirik Mollnes Nathan E. Lewis Victor Nizet Gustav Vaaje-Kolstad
author_facet	Fatemeh Askarian Satoshi Uchiyama Helen Masson Henrik Vinther Sørensen Ole Golten Anne Cathrine Bunæs Sophanit Mekasha Åsmund Kjendseth Røhr Eirik Kommedal Judith Anita Ludviksen Magnus Ø. Arntzen Benjamin Schmidt Raymond H. Zurich Nina M. van Sorge Vincent G. H. Eijsink Ute Krengel Tom Eirik Mollnes Nathan E. Lewis Victor Nizet Gustav Vaaje-Kolstad
author_sort	Fatemeh Askarian
title	The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection
title_short	The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection
title_full	The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection
title_fullStr	The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection
title_full_unstemmed	The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection
title_sort	lytic polysaccharide monooxygenase cbpd promotes pseudomonas aeruginosa virulence in systemic infection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3489cf0fa4cd4b18b05a1a1e7e7583d6
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The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection

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