Direct Current Electrical Fields Improve Experimental Wound Healing by Activation of Cytokine Secretion and Erk1/2 Pathway Stimulation

There is growing evidence that cell behaviors can be influenced by the direct current electric fields (EFs). Some behaviors may influence wound healing directly. This study aimed to investigate the effects of EF (200 mV/mm) on immortalized nontumorigenic human epidermal (HaCaT) cells. We established...

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Autores principales:	Chao Lu, Jonas Kolbenschlag, Andreas K. Nüssler, Sabrina Ehnert, Colin D. McCaig, Urška Čebron, Adrien Daigeler, Cosima Prahm
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	direct current electrical fields HaCaT cells wound healing electrical stimulation Science Q
Acceso en línea:	https://doaj.org/article/348a699befaa410181f232c354705c31
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spelling	oai:doaj.org-article:348a699befaa410181f232c354705c312021-11-25T18:11:05ZDirect Current Electrical Fields Improve Experimental Wound Healing by Activation of Cytokine Secretion and Erk1/2 Pathway Stimulation10.3390/life111111952075-1729https://doaj.org/article/348a699befaa410181f232c354705c312021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1195https://doaj.org/toc/2075-1729There is growing evidence that cell behaviors can be influenced by the direct current electric fields (EFs). Some behaviors may influence wound healing directly. This study aimed to investigate the effects of EF (200 mV/mm) on immortalized nontumorigenic human epidermal (HaCaT) cells. We established a setup that can transmit an EF and maintain a stable cell culture environment. An EF was applied to HaCaT cells, and scratch-assays were performed as a model of wound healing to observe cell migration. Proliferation was evaluated by mitochondrial activity, total protein, and DNA content. Secretion of healing-associated cytokines was evaluated via cytokine arrays, and Western blot was applied to investigate signaling pathway alterations. Compared with the control group, the migration of cells exposed to EFs significantly increased (<i>p</i> < 0.01). After 7 days, the changes in proliferation also increased significantly (<i>p</i> < 0.05). The cytokine arrays revealed that granulocyte-macrophage colony-stimulating factor (GM-CSF) was the most abundant factor secreted by HaCaT following EF exposure. The signals for phospho-Erk1/2 showed a significant (<i>p</i> < 0.0001) increase following EF exposure. The results demonstrate that exposure of HaCaT cells to EFs has positive effects on migration, proliferation, and cytokine secretion—three important steps in wound healing—and these effects may be partially mediated by activation of the Erk1/2 signaling pathway.Chao LuJonas KolbenschlagAndreas K. NüsslerSabrina EhnertColin D. McCaigUrška ČebronAdrien DaigelerCosima PrahmMDPI AGarticledirect current electrical fieldsHaCaT cellswound healingelectrical stimulationScienceQENLife, Vol 11, Iss 1195, p 1195 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	direct current electrical fields HaCaT cells wound healing electrical stimulation Science Q
spellingShingle	direct current electrical fields HaCaT cells wound healing electrical stimulation Science Q Chao Lu Jonas Kolbenschlag Andreas K. Nüssler Sabrina Ehnert Colin D. McCaig Urška Čebron Adrien Daigeler Cosima Prahm Direct Current Electrical Fields Improve Experimental Wound Healing by Activation of Cytokine Secretion and Erk1/2 Pathway Stimulation
description	There is growing evidence that cell behaviors can be influenced by the direct current electric fields (EFs). Some behaviors may influence wound healing directly. This study aimed to investigate the effects of EF (200 mV/mm) on immortalized nontumorigenic human epidermal (HaCaT) cells. We established a setup that can transmit an EF and maintain a stable cell culture environment. An EF was applied to HaCaT cells, and scratch-assays were performed as a model of wound healing to observe cell migration. Proliferation was evaluated by mitochondrial activity, total protein, and DNA content. Secretion of healing-associated cytokines was evaluated via cytokine arrays, and Western blot was applied to investigate signaling pathway alterations. Compared with the control group, the migration of cells exposed to EFs significantly increased (<i>p</i> < 0.01). After 7 days, the changes in proliferation also increased significantly (<i>p</i> < 0.05). The cytokine arrays revealed that granulocyte-macrophage colony-stimulating factor (GM-CSF) was the most abundant factor secreted by HaCaT following EF exposure. The signals for phospho-Erk1/2 showed a significant (<i>p</i> < 0.0001) increase following EF exposure. The results demonstrate that exposure of HaCaT cells to EFs has positive effects on migration, proliferation, and cytokine secretion—three important steps in wound healing—and these effects may be partially mediated by activation of the Erk1/2 signaling pathway.
format	article
author	Chao Lu Jonas Kolbenschlag Andreas K. Nüssler Sabrina Ehnert Colin D. McCaig Urška Čebron Adrien Daigeler Cosima Prahm
author_facet	Chao Lu Jonas Kolbenschlag Andreas K. Nüssler Sabrina Ehnert Colin D. McCaig Urška Čebron Adrien Daigeler Cosima Prahm
author_sort	Chao Lu
title	Direct Current Electrical Fields Improve Experimental Wound Healing by Activation of Cytokine Secretion and Erk1/2 Pathway Stimulation
title_short	Direct Current Electrical Fields Improve Experimental Wound Healing by Activation of Cytokine Secretion and Erk1/2 Pathway Stimulation
title_full	Direct Current Electrical Fields Improve Experimental Wound Healing by Activation of Cytokine Secretion and Erk1/2 Pathway Stimulation
title_fullStr	Direct Current Electrical Fields Improve Experimental Wound Healing by Activation of Cytokine Secretion and Erk1/2 Pathway Stimulation
title_full_unstemmed	Direct Current Electrical Fields Improve Experimental Wound Healing by Activation of Cytokine Secretion and Erk1/2 Pathway Stimulation
title_sort	direct current electrical fields improve experimental wound healing by activation of cytokine secretion and erk1/2 pathway stimulation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/348a699befaa410181f232c354705c31
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Direct Current Electrical Fields Improve Experimental Wound Healing by Activation of Cytokine Secretion and Erk1/2 Pathway Stimulation

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