Design of near-infrared fluorescent bioactive conjugated functional iron oxide nanoparticles for optical detection of colon cancer

Enav Corem-Salkmon, Benny Perlstein, Shlomo MargelThe Institute of Nanotechnology and Advanced Materials, Department of Chemistry, Bar-Ilan University, Ramat-Gan, IsraelBackground: Colon cancer is one of the major causes of death in the Western world. Early detection significantly improves long-term...

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Autores principales:	Corem-Salkmon E, Perlstein B, Margel S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/348eb065998d489a99bd9f553f597cf6
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spelling	oai:doaj.org-article:348eb065998d489a99bd9f553f597cf62021-12-02T05:40:40ZDesign of near-infrared fluorescent bioactive conjugated functional iron oxide nanoparticles for optical detection of colon cancer1176-91141178-2013https://doaj.org/article/348eb065998d489a99bd9f553f597cf62012-10-01T00:00:00Zhttp://www.dovepress.com/design-of-near-infrared-fluorescent-bioactive-conjugated-functional-ir-a11320https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Enav Corem-Salkmon, Benny Perlstein, Shlomo MargelThe Institute of Nanotechnology and Advanced Materials, Department of Chemistry, Bar-Ilan University, Ramat-Gan, IsraelBackground: Colon cancer is one of the major causes of death in the Western world. Early detection significantly improves long-term survival for patients with the disease. Near-infrared (NIR) fluorescent nanoparticles hold great promise as contrast agents for tumor detection. NIR offers several advantages for bioimaging compared with fluorescence in the visible spectrum, ie, lower autofluorescence of biological tissues, lower absorbance, and consequently deeper penetration into biomatrices.Methods and results: NIR fluorescent iron oxide nanoparticles with a narrow size distribution were prepared by nucleation, followed by controlled growth of thin iron oxide films onto cyanine NIR dye conjugated gelatin-iron oxide nuclei. For functionalization, and in order to increase the NIR fluorescence intensity, the NIR fluorescent iron oxide nanoparticles obtained were coated with human serum albumin containing cyanine NIR dye. Leakage of the NIR dye from these nanoparticles into phosphate-buffered saline solution containing 4% albumin was not detected. The work presented here is a feasibility study to test the suitability of iron oxide-human serum albumin NIR fluorescent nanoparticles for optical detection of colon cancer. It demonstrates that encapsulation of NIR fluorescent dye within these nanoparticles significantly reduces photobleaching of the dye. Tumor-targeting ligands, peanut agglutinin and anticarcinoembryonic antigen antibodies (&alpha;CEA), were covalently conjugated with the NIR fluorescent iron oxide-human serum albumin nanoparticles via a poly(ethylene glycol) spacer. Specific colon tumor detection was demonstrated in chicken embryo and mouse models for both nonconjugated and the peanut agglutinin-conjugated or &alpha;CEA-conjugated NIR fluorescent iron oxide-human serum albumin nanoparticles.Conclusion: Conjugation of peanut agglutinin or &alpha;CEA to the nanoparticles significantly increased the fluorescence intensity of the tagged colon tumor tissues relative to the nonconjugated nanoparticles.Keywords: near-infrared fluorescence, iron oxide nanoparticles, optical imaging, chorioallantoic membrane assay, colon cancerCorem-Salkmon EPerlstein BMargel SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5517-5527 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Corem-Salkmon E Perlstein B Margel S Design of near-infrared fluorescent bioactive conjugated functional iron oxide nanoparticles for optical detection of colon cancer
description	Enav Corem-Salkmon, Benny Perlstein, Shlomo MargelThe Institute of Nanotechnology and Advanced Materials, Department of Chemistry, Bar-Ilan University, Ramat-Gan, IsraelBackground: Colon cancer is one of the major causes of death in the Western world. Early detection significantly improves long-term survival for patients with the disease. Near-infrared (NIR) fluorescent nanoparticles hold great promise as contrast agents for tumor detection. NIR offers several advantages for bioimaging compared with fluorescence in the visible spectrum, ie, lower autofluorescence of biological tissues, lower absorbance, and consequently deeper penetration into biomatrices.Methods and results: NIR fluorescent iron oxide nanoparticles with a narrow size distribution were prepared by nucleation, followed by controlled growth of thin iron oxide films onto cyanine NIR dye conjugated gelatin-iron oxide nuclei. For functionalization, and in order to increase the NIR fluorescence intensity, the NIR fluorescent iron oxide nanoparticles obtained were coated with human serum albumin containing cyanine NIR dye. Leakage of the NIR dye from these nanoparticles into phosphate-buffered saline solution containing 4% albumin was not detected. The work presented here is a feasibility study to test the suitability of iron oxide-human serum albumin NIR fluorescent nanoparticles for optical detection of colon cancer. It demonstrates that encapsulation of NIR fluorescent dye within these nanoparticles significantly reduces photobleaching of the dye. Tumor-targeting ligands, peanut agglutinin and anticarcinoembryonic antigen antibodies (&alpha;CEA), were covalently conjugated with the NIR fluorescent iron oxide-human serum albumin nanoparticles via a poly(ethylene glycol) spacer. Specific colon tumor detection was demonstrated in chicken embryo and mouse models for both nonconjugated and the peanut agglutinin-conjugated or &alpha;CEA-conjugated NIR fluorescent iron oxide-human serum albumin nanoparticles.Conclusion: Conjugation of peanut agglutinin or &alpha;CEA to the nanoparticles significantly increased the fluorescence intensity of the tagged colon tumor tissues relative to the nonconjugated nanoparticles.Keywords: near-infrared fluorescence, iron oxide nanoparticles, optical imaging, chorioallantoic membrane assay, colon cancer
format	article
author	Corem-Salkmon E Perlstein B Margel S
author_facet	Corem-Salkmon E Perlstein B Margel S
author_sort	Corem-Salkmon E
title	Design of near-infrared fluorescent bioactive conjugated functional iron oxide nanoparticles for optical detection of colon cancer
title_short	Design of near-infrared fluorescent bioactive conjugated functional iron oxide nanoparticles for optical detection of colon cancer
title_full	Design of near-infrared fluorescent bioactive conjugated functional iron oxide nanoparticles for optical detection of colon cancer
title_fullStr	Design of near-infrared fluorescent bioactive conjugated functional iron oxide nanoparticles for optical detection of colon cancer
title_full_unstemmed	Design of near-infrared fluorescent bioactive conjugated functional iron oxide nanoparticles for optical detection of colon cancer
title_sort	design of near-infrared fluorescent bioactive conjugated functional iron oxide nanoparticles for optical detection of colon cancer
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/348eb065998d489a99bd9f553f597cf6
work_keys_str_mv	AT coremsalkmone designofnearinfraredfluorescentbioactiveconjugatedfunctionalironoxidenanoparticlesforopticaldetectionofcoloncancer AT perlsteinb designofnearinfraredfluorescentbioactiveconjugatedfunctionalironoxidenanoparticlesforopticaldetectionofcoloncancer AT margels designofnearinfraredfluorescentbioactiveconjugatedfunctionalironoxidenanoparticlesforopticaldetectionofcoloncancer
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Design of near-infrared fluorescent bioactive conjugated functional iron oxide nanoparticles for optical detection of colon cancer

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