Relationships between TGFbeta proteins and oxygen concentrations inside the first trimester human gestational sac.

In early pregnancy, the O(2) gradient between the maternal circulation and the gestational sac tissues modulates trophoblast biological functions. The aim was to evaluate if placental partial pressure of oxygen (PaO(2)) modulates in vivo synthesis of specific placental proteins inside the first trim...

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Autores principales:	Shanthi Muttukrishna, Sangeeta Suri, Nigel Groome, Eric Jauniaux
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3490a806349441bda1e050b55f913040
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spelling	oai:doaj.org-article:3490a806349441bda1e050b55f9130402021-11-25T06:12:09ZRelationships between TGFbeta proteins and oxygen concentrations inside the first trimester human gestational sac.1932-620310.1371/journal.pone.0002302https://doaj.org/article/3490a806349441bda1e050b55f9130402008-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18523681/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In early pregnancy, the O(2) gradient between the maternal circulation and the gestational sac tissues modulates trophoblast biological functions. The aim was to evaluate if placental partial pressure of oxygen (PaO(2)) modulates in vivo synthesis of specific placental proteins inside the first trimester gestational sac. Matched samples of peripheral venous blood, blood from the placental bed (PB), coelomic fluid (CF) and placental tissue were obtained in 37 normal pregnancies at 6-12 weeks gestation. PaO(2) was measured in PB and CF using an IRMA blood gas monitor. Inhibin A, activin A, sEng, PlGF, sFlt-1 and free VEGF concentrations were measured in all samples. HSP 70 was measured in placental extracts. ANOVA showed approximately 60% increase in PB PaO(2) (P = 0.02) between after 10 weeks gestation. Unpaired Student's T-test between two groups (6-9 weeks vs 9-12 weeks) shows a significant increase in MS Activin A (P = 0.001), CF activin A (P<0.001), MS P1GF (P = 0.001), CF PlGF (P<0.001), MS sFLT-1 (P = 0.03), CF sFLT-1 (P = 0.01), HSP 70 in placental extracts (P = 0.04) and a significant decrease in PB inhibin A levels (P<0.001) and PB sFLT-1 (P = 0.02) . Multiple correlation analysis showed a significant negative correlation between PB inhibin A levels and gestation (r = -0.45, P<0.05) and PB PaO(2) (r = -0.5, P = 0.008) and also between sFLT-1 and PB PaO(2) (P = 0.03). There was a positive correlation (P<0.01) between PlGF, sEng and VEGF levels in the placental extracts. Our results indicate a direct relationship in the early intrauterine PaO(2) in vivo and inhibin A and sFLT-1 concentrations confirming our hypothesis that specific placental proteins are regulated by intrauterine O(2) tension.Shanthi MuttukrishnaSangeeta SuriNigel GroomeEric JauniauxPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 6, p e2302 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shanthi Muttukrishna Sangeeta Suri Nigel Groome Eric Jauniaux Relationships between TGFbeta proteins and oxygen concentrations inside the first trimester human gestational sac.
description	In early pregnancy, the O(2) gradient between the maternal circulation and the gestational sac tissues modulates trophoblast biological functions. The aim was to evaluate if placental partial pressure of oxygen (PaO(2)) modulates in vivo synthesis of specific placental proteins inside the first trimester gestational sac. Matched samples of peripheral venous blood, blood from the placental bed (PB), coelomic fluid (CF) and placental tissue were obtained in 37 normal pregnancies at 6-12 weeks gestation. PaO(2) was measured in PB and CF using an IRMA blood gas monitor. Inhibin A, activin A, sEng, PlGF, sFlt-1 and free VEGF concentrations were measured in all samples. HSP 70 was measured in placental extracts. ANOVA showed approximately 60% increase in PB PaO(2) (P = 0.02) between after 10 weeks gestation. Unpaired Student's T-test between two groups (6-9 weeks vs 9-12 weeks) shows a significant increase in MS Activin A (P = 0.001), CF activin A (P<0.001), MS P1GF (P = 0.001), CF PlGF (P<0.001), MS sFLT-1 (P = 0.03), CF sFLT-1 (P = 0.01), HSP 70 in placental extracts (P = 0.04) and a significant decrease in PB inhibin A levels (P<0.001) and PB sFLT-1 (P = 0.02) . Multiple correlation analysis showed a significant negative correlation between PB inhibin A levels and gestation (r = -0.45, P<0.05) and PB PaO(2) (r = -0.5, P = 0.008) and also between sFLT-1 and PB PaO(2) (P = 0.03). There was a positive correlation (P<0.01) between PlGF, sEng and VEGF levels in the placental extracts. Our results indicate a direct relationship in the early intrauterine PaO(2) in vivo and inhibin A and sFLT-1 concentrations confirming our hypothesis that specific placental proteins are regulated by intrauterine O(2) tension.
format	article
author	Shanthi Muttukrishna Sangeeta Suri Nigel Groome Eric Jauniaux
author_facet	Shanthi Muttukrishna Sangeeta Suri Nigel Groome Eric Jauniaux
author_sort	Shanthi Muttukrishna
title	Relationships between TGFbeta proteins and oxygen concentrations inside the first trimester human gestational sac.
title_short	Relationships between TGFbeta proteins and oxygen concentrations inside the first trimester human gestational sac.
title_full	Relationships between TGFbeta proteins and oxygen concentrations inside the first trimester human gestational sac.
title_fullStr	Relationships between TGFbeta proteins and oxygen concentrations inside the first trimester human gestational sac.
title_full_unstemmed	Relationships between TGFbeta proteins and oxygen concentrations inside the first trimester human gestational sac.
title_sort	relationships between tgfbeta proteins and oxygen concentrations inside the first trimester human gestational sac.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/3490a806349441bda1e050b55f913040
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Relationships between TGFbeta proteins and oxygen concentrations inside the first trimester human gestational sac.

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