Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates

Abstract Brucella canis infection is an underdiagnosed zoonotic disease. Knowledge about perinatal brucellosis in dogs is extremely limited, although foetuses and neonates are under risk of infection due to vertical transmission. In this study, immunohistochemistry was used to determine tissue distr...

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Autores principales:	Tayse Domingues de Souza, Tatiane Furtado de Carvalho, Juliana Pinto da Silva Mol, João Vítor Menezes Lopes, Monique Ferreira Silva, Tatiane Alves da Paixão, Renato Lima Santos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/349ea1a0071d40828628eaa2a49dac69
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spelling	oai:doaj.org-article:349ea1a0071d40828628eaa2a49dac692021-12-02T12:32:36ZTissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates10.1038/s41598-018-25651-x2045-2322https://doaj.org/article/349ea1a0071d40828628eaa2a49dac692018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25651-xhttps://doaj.org/toc/2045-2322Abstract Brucella canis infection is an underdiagnosed zoonotic disease. Knowledge about perinatal brucellosis in dogs is extremely limited, although foetuses and neonates are under risk of infection due to vertical transmission. In this study, immunohistochemistry was used to determine tissue distribution and cell tropism of B. canis in canine foetuses and neonates. Diagnosis of B. canis in tissues of naturally infected pups was based on PCR and sequencing of amplicons, bacterial isolation, and immunohistochemistry, whose specificity was confirmed by laser capture microdissection. PCR positivity among 200 puppies was 21%, and nine isolates of B. canis were obtained. Tissues from 13 PCR-positive puppies (4 stillborn and 9 neonates) presented widespread immunolabeling. Stomach, intestines, kidney, nervous system, and umbilicus were positive in all animals tested. Other frequently infected organs included the liver (92%), lungs (85%), lymph nodes (69%), and spleen (62%). Immunolabeled coccobacilli occurred mostly in macrophages, but they were also observed in erythrocytes, epithelial cells of gastrointestinal mucosa, renal tubules, epidermis, adipocytes, choroid plexus, ependyma, neuroblasts, blood vessels endothelium, muscle cells, and in the intestinal lumen. These results largely expand our knowledge about perinatal brucellosis in the dog, clearly demonstrating a pantropic distribution of B. canis in naturally infected foetuses and neonates.Tayse Domingues de SouzaTatiane Furtado de CarvalhoJuliana Pinto da Silva MolJoão Vítor Menezes LopesMonique Ferreira SilvaTatiane Alves da PaixãoRenato Lima SantosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tayse Domingues de Souza Tatiane Furtado de Carvalho Juliana Pinto da Silva Mol João Vítor Menezes Lopes Monique Ferreira Silva Tatiane Alves da Paixão Renato Lima Santos Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates
description	Abstract Brucella canis infection is an underdiagnosed zoonotic disease. Knowledge about perinatal brucellosis in dogs is extremely limited, although foetuses and neonates are under risk of infection due to vertical transmission. In this study, immunohistochemistry was used to determine tissue distribution and cell tropism of B. canis in canine foetuses and neonates. Diagnosis of B. canis in tissues of naturally infected pups was based on PCR and sequencing of amplicons, bacterial isolation, and immunohistochemistry, whose specificity was confirmed by laser capture microdissection. PCR positivity among 200 puppies was 21%, and nine isolates of B. canis were obtained. Tissues from 13 PCR-positive puppies (4 stillborn and 9 neonates) presented widespread immunolabeling. Stomach, intestines, kidney, nervous system, and umbilicus were positive in all animals tested. Other frequently infected organs included the liver (92%), lungs (85%), lymph nodes (69%), and spleen (62%). Immunolabeled coccobacilli occurred mostly in macrophages, but they were also observed in erythrocytes, epithelial cells of gastrointestinal mucosa, renal tubules, epidermis, adipocytes, choroid plexus, ependyma, neuroblasts, blood vessels endothelium, muscle cells, and in the intestinal lumen. These results largely expand our knowledge about perinatal brucellosis in the dog, clearly demonstrating a pantropic distribution of B. canis in naturally infected foetuses and neonates.
format	article
author	Tayse Domingues de Souza Tatiane Furtado de Carvalho Juliana Pinto da Silva Mol João Vítor Menezes Lopes Monique Ferreira Silva Tatiane Alves da Paixão Renato Lima Santos
author_facet	Tayse Domingues de Souza Tatiane Furtado de Carvalho Juliana Pinto da Silva Mol João Vítor Menezes Lopes Monique Ferreira Silva Tatiane Alves da Paixão Renato Lima Santos
author_sort	Tayse Domingues de Souza
title	Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates
title_short	Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates
title_full	Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates
title_fullStr	Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates
title_full_unstemmed	Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates
title_sort	tissue distribution and cell tropism of brucella canis in naturally infected canine foetuses and neonates
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/349ea1a0071d40828628eaa2a49dac69
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Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates

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