Vascular Polyurethane Prostheses Modified with a Bioactive Coating—Physicochemical, Mechanical and Biological Properties

This study describes a method for the modification of polyurethane small-diameter (5 mm) vascular prostheses obtained with the phase inversion method. The modification process involves two steps: the introduction of a linker (acrylic acid) and a peptide (REDV and YIGSR). FTIR and XPS analysis confir...

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Autores principales:	Aleksandra Kuźmińska, Aleksandra Wojciechowska, Beata A. Butruk-Raszeja
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	phase-inversion technique vascular prosthesis polyurethane surface modification hemocompatibility endothelial cell Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/34a40219800b4b81a5613f02685a2e5d
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spelling	oai:doaj.org-article:34a40219800b4b81a5613f02685a2e5d2021-11-25T17:54:05ZVascular Polyurethane Prostheses Modified with a Bioactive Coating—Physicochemical, Mechanical and Biological Properties10.3390/ijms2222121831422-00671661-6596https://doaj.org/article/34a40219800b4b81a5613f02685a2e5d2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12183https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067This study describes a method for the modification of polyurethane small-diameter (5 mm) vascular prostheses obtained with the phase inversion method. The modification process involves two steps: the introduction of a linker (acrylic acid) and a peptide (REDV and YIGSR). FTIR and XPS analysis confirmed the process of chemical modification. The obtained prostheses had a porosity of approx. 60%, Young’s Modulus in the range of 9–11 MPa, and a water contact angle around 40°. Endothelial (EC) and smooth muscle (SMC) cell co-culture showed that the surfaces modified with peptides increase the adhesion of ECs. At the same time, SMCs adhesion was low both on unmodified and peptide-modified surfaces. Analysis of blood-materials interaction showed high hemocompatibility of obtained materials. The whole blood clotting time assay showed differences in the amount of free hemoglobin present in blood contacted with different materials. It can be concluded that the peptide coating increased the hemocompatibility of the surface by increasing ECs adhesion and, at the same time, decreasing platelet adhesion. When comparing both types of peptide coatings, more promising results were obtained for the surfaces coated with the YISGR than REDV-coated prostheses.Aleksandra KuźmińskaAleksandra WojciechowskaBeata A. Butruk-RaszejaMDPI AGarticlephase-inversion techniquevascular prosthesispolyurethanesurface modificationhemocompatibilityendothelial cellBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12183, p 12183 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	phase-inversion technique vascular prosthesis polyurethane surface modification hemocompatibility endothelial cell Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	phase-inversion technique vascular prosthesis polyurethane surface modification hemocompatibility endothelial cell Biology (General) QH301-705.5 Chemistry QD1-999 Aleksandra Kuźmińska Aleksandra Wojciechowska Beata A. Butruk-Raszeja Vascular Polyurethane Prostheses Modified with a Bioactive Coating—Physicochemical, Mechanical and Biological Properties
description	This study describes a method for the modification of polyurethane small-diameter (5 mm) vascular prostheses obtained with the phase inversion method. The modification process involves two steps: the introduction of a linker (acrylic acid) and a peptide (REDV and YIGSR). FTIR and XPS analysis confirmed the process of chemical modification. The obtained prostheses had a porosity of approx. 60%, Young’s Modulus in the range of 9–11 MPa, and a water contact angle around 40°. Endothelial (EC) and smooth muscle (SMC) cell co-culture showed that the surfaces modified with peptides increase the adhesion of ECs. At the same time, SMCs adhesion was low both on unmodified and peptide-modified surfaces. Analysis of blood-materials interaction showed high hemocompatibility of obtained materials. The whole blood clotting time assay showed differences in the amount of free hemoglobin present in blood contacted with different materials. It can be concluded that the peptide coating increased the hemocompatibility of the surface by increasing ECs adhesion and, at the same time, decreasing platelet adhesion. When comparing both types of peptide coatings, more promising results were obtained for the surfaces coated with the YISGR than REDV-coated prostheses.
format	article
author	Aleksandra Kuźmińska Aleksandra Wojciechowska Beata A. Butruk-Raszeja
author_facet	Aleksandra Kuźmińska Aleksandra Wojciechowska Beata A. Butruk-Raszeja
author_sort	Aleksandra Kuźmińska
title	Vascular Polyurethane Prostheses Modified with a Bioactive Coating—Physicochemical, Mechanical and Biological Properties
title_short	Vascular Polyurethane Prostheses Modified with a Bioactive Coating—Physicochemical, Mechanical and Biological Properties
title_full	Vascular Polyurethane Prostheses Modified with a Bioactive Coating—Physicochemical, Mechanical and Biological Properties
title_fullStr	Vascular Polyurethane Prostheses Modified with a Bioactive Coating—Physicochemical, Mechanical and Biological Properties
title_full_unstemmed	Vascular Polyurethane Prostheses Modified with a Bioactive Coating—Physicochemical, Mechanical and Biological Properties
title_sort	vascular polyurethane prostheses modified with a bioactive coating—physicochemical, mechanical and biological properties
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/34a40219800b4b81a5613f02685a2e5d
work_keys_str_mv	AT aleksandrakuzminska vascularpolyurethaneprosthesesmodifiedwithabioactivecoatingphysicochemicalmechanicalandbiologicalproperties AT aleksandrawojciechowska vascularpolyurethaneprosthesesmodifiedwithabioactivecoatingphysicochemicalmechanicalandbiologicalproperties AT beataabutrukraszeja vascularpolyurethaneprosthesesmodifiedwithabioactivecoatingphysicochemicalmechanicalandbiologicalproperties
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Vascular Polyurethane Prostheses Modified with a Bioactive Coating—Physicochemical, Mechanical and Biological Properties

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