Rat bone marrow-derived Schwann-like cells differentiated by the optimal inducers combination on microfluidic chip and their functional performance.

Numerous researches demonstrated the possibility of derivation of Schwann-like (SC-like) cells in vitro from bone marrow stromal cells (BMSCs). However, the concentration of the induce factors were different in those studies, especially for the critical factors forskolin (FSK) and β-heregulin (HRG)....

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Autores principales:	Xiliang Tian, Shouyu Wang, Zhen Zhang, Decheng Lv
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/34a548793da24153a107b070c7d0c397
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spelling	oai:doaj.org-article:34a548793da24153a107b070c7d0c3972021-11-18T07:09:38ZRat bone marrow-derived Schwann-like cells differentiated by the optimal inducers combination on microfluidic chip and their functional performance.1932-620310.1371/journal.pone.0042804https://doaj.org/article/34a548793da24153a107b070c7d0c3972012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22880114/?tool=EBIhttps://doaj.org/toc/1932-6203Numerous researches demonstrated the possibility of derivation of Schwann-like (SC-like) cells in vitro from bone marrow stromal cells (BMSCs). However, the concentration of the induce factors were different in those studies, especially for the critical factors forskolin (FSK) and β-heregulin (HRG). Here, we used a new and useful method to build an integrated microfluidic chip for rapid analyses of the optimal combination between the induce factors FSK and HRG. The microfluidic device was mainly composed of an upstream concentration gradient generator (CGG) and a downstream cell culture module. Rat BMSCs were cultured in the cell chambers for 11 days at the different concentrations of induce factors generated by CGG. The result of immunofluorescence staining on-chip showed that the group of 4.00 µM FSK and 250.00 ng/ml HRG presented an optimal effect to promote the derivation of SC-like cells. Moreover, the optimal SC-like cells obtained on-chip were further tested using DRG co-culture and ELISA to detect their functional performance. Our findings demonstrate that SC-like cells could be obtained with high efficiency and functional performance in the optimal inducers combination.Xiliang TianShouyu WangZhen ZhangDecheng LvPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42804 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiliang Tian Shouyu Wang Zhen Zhang Decheng Lv Rat bone marrow-derived Schwann-like cells differentiated by the optimal inducers combination on microfluidic chip and their functional performance.
description	Numerous researches demonstrated the possibility of derivation of Schwann-like (SC-like) cells in vitro from bone marrow stromal cells (BMSCs). However, the concentration of the induce factors were different in those studies, especially for the critical factors forskolin (FSK) and β-heregulin (HRG). Here, we used a new and useful method to build an integrated microfluidic chip for rapid analyses of the optimal combination between the induce factors FSK and HRG. The microfluidic device was mainly composed of an upstream concentration gradient generator (CGG) and a downstream cell culture module. Rat BMSCs were cultured in the cell chambers for 11 days at the different concentrations of induce factors generated by CGG. The result of immunofluorescence staining on-chip showed that the group of 4.00 µM FSK and 250.00 ng/ml HRG presented an optimal effect to promote the derivation of SC-like cells. Moreover, the optimal SC-like cells obtained on-chip were further tested using DRG co-culture and ELISA to detect their functional performance. Our findings demonstrate that SC-like cells could be obtained with high efficiency and functional performance in the optimal inducers combination.
format	article
author	Xiliang Tian Shouyu Wang Zhen Zhang Decheng Lv
author_facet	Xiliang Tian Shouyu Wang Zhen Zhang Decheng Lv
author_sort	Xiliang Tian
title	Rat bone marrow-derived Schwann-like cells differentiated by the optimal inducers combination on microfluidic chip and their functional performance.
title_short	Rat bone marrow-derived Schwann-like cells differentiated by the optimal inducers combination on microfluidic chip and their functional performance.
title_full	Rat bone marrow-derived Schwann-like cells differentiated by the optimal inducers combination on microfluidic chip and their functional performance.
title_fullStr	Rat bone marrow-derived Schwann-like cells differentiated by the optimal inducers combination on microfluidic chip and their functional performance.
title_full_unstemmed	Rat bone marrow-derived Schwann-like cells differentiated by the optimal inducers combination on microfluidic chip and their functional performance.
title_sort	rat bone marrow-derived schwann-like cells differentiated by the optimal inducers combination on microfluidic chip and their functional performance.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/34a548793da24153a107b070c7d0c397
work_keys_str_mv	AT xiliangtian ratbonemarrowderivedschwannlikecellsdifferentiatedbytheoptimalinducerscombinationonmicrofluidicchipandtheirfunctionalperformance AT shouyuwang ratbonemarrowderivedschwannlikecellsdifferentiatedbytheoptimalinducerscombinationonmicrofluidicchipandtheirfunctionalperformance AT zhenzhang ratbonemarrowderivedschwannlikecellsdifferentiatedbytheoptimalinducerscombinationonmicrofluidicchipandtheirfunctionalperformance AT dechenglv ratbonemarrowderivedschwannlikecellsdifferentiatedbytheoptimalinducerscombinationonmicrofluidicchipandtheirfunctionalperformance
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Rat bone marrow-derived Schwann-like cells differentiated by the optimal inducers combination on microfluidic chip and their functional performance.

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