The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury

Fonny Josh,1,2 Tomie Hermawan Soekamto,3 Januar Rizky Adriani,4 Billy Jonatan,4 Hiroshi Mizuno,5 Muhammad Faruk4 1Division of Plastic and Reconstructive Surgery, Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia; 2Department of Plastic and Reconst...

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Autores principales:	Josh F, Soekamto TH, Adriani JR, Jonatan B, Mizuno H, Faruk M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	stromal vascular fraction cells platelet rich plasma malondialdehyde nitric oxide burn injury Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/34a673c3a3bc4e44b69deb3206d39944
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spelling	oai:doaj.org-article:34a673c3a3bc4e44b69deb3206d399442021-12-02T16:15:13ZThe Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury1178-7031https://doaj.org/article/34a673c3a3bc4e44b69deb3206d399442021-07-01T00:00:00Zhttps://www.dovepress.com/the-combination-of-stromal-vascular-fraction-cells-and-platelet-rich-p-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Fonny Josh,1,2 Tomie Hermawan Soekamto,3 Januar Rizky Adriani,4 Billy Jonatan,4 Hiroshi Mizuno,5 Muhammad Faruk4 1Division of Plastic and Reconstructive Surgery, Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia; 2Department of Plastic and Reconstructive Surgery, Wahidin Sudirohusodo Hospital, Makassar, South Sulawesi, Indonesia; 3Department of Plastic and Reconstructive Surgery, Koja Hospital, Jakarta, Indonesia; 4Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia; 5Department of Plastic and Reconstructive Surgery, Juntendo University, Tokyo, JapanCorrespondence: Fonny JoshDivision of Plastic and Reconstructive Surgery, Department of Surgery, Faculty of Medicine, Hasanuddin University, Jalan Perintis Kemerdekaan KM 11, Makassar, 90245, IndonesiaFax +62411585984Email fonnyjosh2003@yahoo.comIntroduction: Thermal burns release reactive oxygen species, which cause profound systemic and local changes. Stromal vascular fraction cells (SVFs) combined with platelet-rich plasma accelerate burn wound healing. This study investigated the effect of a combination of locally injected SVFs and PRP on malondialdehyde (MDA) and nitric oxide (NO) serum and tissue levels in a deep dermal burn model in Wistar rats.Methods: Thirty-six adult Wistar rats weighing between 150 and 250 grams were used in this study to establish a deep dermal degree burn wound model. They were randomly divided into 4 groups: locally injected the combination SVFs and PRP, the Vaseline group, the placebo group, and healthy Wistar rats (the normal control group). MDA and NO levels in blood serum and burn wound tissue were measured at 8, 24, and 48 hours. Data were analyzed with one-way ANOVA followed by multiple comparisons tests and regression tests.Results: Local injection of SVFs and PRP in combination affected blood MDA, tissue MDA, blood NO and tissue NO levels, with reductions of 0.257μmol/L, 0.427 μmol/L, 21.78nmol/mg, and 23.777nmol/mg, respectively. Injection of SVFs and PRP in combination reduced tissue MDA levels by 1.282 times, NO blood levels by 2.305, and NO tissue levels by 2.377 times compared to Vaseline application.Conclusion: The combination of SVFs and PRP undeniably reduced the MDA and NO levels in blood and tissue compared to those in the Vaseline and placebo groups. The injection of these two preparations in combination inhibited the local and systemic stress oxidative response, as illustrated by the decreased MDA and NO levels in blood serum and tissue.Keywords: stromal vascular fraction cells, platelet rich plasma, malondialdehyde, nitric oxide, burn injuryJosh FSoekamto THAdriani JRJonatan BMizuno HFaruk MDove Medical Pressarticlestromal vascular fraction cellsplatelet rich plasmamalondialdehydenitric oxideburn injuryPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 3049-3061 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	stromal vascular fraction cells platelet rich plasma malondialdehyde nitric oxide burn injury Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	stromal vascular fraction cells platelet rich plasma malondialdehyde nitric oxide burn injury Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Josh F Soekamto TH Adriani JR Jonatan B Mizuno H Faruk M The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury
description	Fonny Josh,1,2 Tomie Hermawan Soekamto,3 Januar Rizky Adriani,4 Billy Jonatan,4 Hiroshi Mizuno,5 Muhammad Faruk4 1Division of Plastic and Reconstructive Surgery, Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia; 2Department of Plastic and Reconstructive Surgery, Wahidin Sudirohusodo Hospital, Makassar, South Sulawesi, Indonesia; 3Department of Plastic and Reconstructive Surgery, Koja Hospital, Jakarta, Indonesia; 4Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia; 5Department of Plastic and Reconstructive Surgery, Juntendo University, Tokyo, JapanCorrespondence: Fonny JoshDivision of Plastic and Reconstructive Surgery, Department of Surgery, Faculty of Medicine, Hasanuddin University, Jalan Perintis Kemerdekaan KM 11, Makassar, 90245, IndonesiaFax +62411585984Email fonnyjosh2003@yahoo.comIntroduction: Thermal burns release reactive oxygen species, which cause profound systemic and local changes. Stromal vascular fraction cells (SVFs) combined with platelet-rich plasma accelerate burn wound healing. This study investigated the effect of a combination of locally injected SVFs and PRP on malondialdehyde (MDA) and nitric oxide (NO) serum and tissue levels in a deep dermal burn model in Wistar rats.Methods: Thirty-six adult Wistar rats weighing between 150 and 250 grams were used in this study to establish a deep dermal degree burn wound model. They were randomly divided into 4 groups: locally injected the combination SVFs and PRP, the Vaseline group, the placebo group, and healthy Wistar rats (the normal control group). MDA and NO levels in blood serum and burn wound tissue were measured at 8, 24, and 48 hours. Data were analyzed with one-way ANOVA followed by multiple comparisons tests and regression tests.Results: Local injection of SVFs and PRP in combination affected blood MDA, tissue MDA, blood NO and tissue NO levels, with reductions of 0.257μmol/L, 0.427 μmol/L, 21.78nmol/mg, and 23.777nmol/mg, respectively. Injection of SVFs and PRP in combination reduced tissue MDA levels by 1.282 times, NO blood levels by 2.305, and NO tissue levels by 2.377 times compared to Vaseline application.Conclusion: The combination of SVFs and PRP undeniably reduced the MDA and NO levels in blood and tissue compared to those in the Vaseline and placebo groups. The injection of these two preparations in combination inhibited the local and systemic stress oxidative response, as illustrated by the decreased MDA and NO levels in blood serum and tissue.Keywords: stromal vascular fraction cells, platelet rich plasma, malondialdehyde, nitric oxide, burn injury
format	article
author	Josh F Soekamto TH Adriani JR Jonatan B Mizuno H Faruk M
author_facet	Josh F Soekamto TH Adriani JR Jonatan B Mizuno H Faruk M
author_sort	Josh F
title	The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury
title_short	The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury
title_full	The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury
title_fullStr	The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury
title_full_unstemmed	The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury
title_sort	combination of stromal vascular fraction cells and platelet-rich plasma reduces malondialdehyde and nitric oxide levels in deep dermal burn injury
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/34a673c3a3bc4e44b69deb3206d39944
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The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury

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