SERRS multiplexing with multivalent nanostructures for the identification and enumeration of epithelial and mesenchymal cells

Abstract Liquid biopsy represents a new frontier of cancer diagnosis and prognosis, which allows the isolation of tumor cells released in the blood stream. The extremely low abundance of these cells needs appropriate methodologies for their identification and enumeration. Herein we present a new pro...

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Autores principales:	Lucio Litti, Andrea Colusso, Marcella Pinto, Erlis Ruli, Alessia Scarsi, Laura Ventura, Giuseppe Toffoli, Marco Colombatti, Giulio Fracasso, Moreno Meneghetti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/34aaa39c17f24ade84f6d894cc073d5f
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spelling	oai:doaj.org-article:34aaa39c17f24ade84f6d894cc073d5f2021-12-02T18:48:08ZSERRS multiplexing with multivalent nanostructures for the identification and enumeration of epithelial and mesenchymal cells10.1038/s41598-020-72911-w2045-2322https://doaj.org/article/34aaa39c17f24ade84f6d894cc073d5f2020-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-72911-whttps://doaj.org/toc/2045-2322Abstract Liquid biopsy represents a new frontier of cancer diagnosis and prognosis, which allows the isolation of tumor cells released in the blood stream. The extremely low abundance of these cells needs appropriate methodologies for their identification and enumeration. Herein we present a new protocol based on surface enhanced resonance Raman scattering (SERRS) gold multivalent nanostructures to identify and enumerate tumor cells with epithelial and mesenchimal markers. The validation of the protocol is obtained with spiked samples of peripheral blood mononuclear cells (PBMC). Gold nanostructures are functionalized with SERRS labels and with antibodies to link the tumor cells. Three types of such nanosystems were simultaneously used and the protocol allows obtaining the identification of all individual tumor cells with the help of a Random Forest ensemble learning method.Lucio LittiAndrea ColussoMarcella PintoErlis RuliAlessia ScarsiLaura VenturaGiuseppe ToffoliMarco ColombattiGiulio FracassoMoreno MeneghettiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucio Litti Andrea Colusso Marcella Pinto Erlis Ruli Alessia Scarsi Laura Ventura Giuseppe Toffoli Marco Colombatti Giulio Fracasso Moreno Meneghetti SERRS multiplexing with multivalent nanostructures for the identification and enumeration of epithelial and mesenchymal cells
description	Abstract Liquid biopsy represents a new frontier of cancer diagnosis and prognosis, which allows the isolation of tumor cells released in the blood stream. The extremely low abundance of these cells needs appropriate methodologies for their identification and enumeration. Herein we present a new protocol based on surface enhanced resonance Raman scattering (SERRS) gold multivalent nanostructures to identify and enumerate tumor cells with epithelial and mesenchimal markers. The validation of the protocol is obtained with spiked samples of peripheral blood mononuclear cells (PBMC). Gold nanostructures are functionalized with SERRS labels and with antibodies to link the tumor cells. Three types of such nanosystems were simultaneously used and the protocol allows obtaining the identification of all individual tumor cells with the help of a Random Forest ensemble learning method.
format	article
author	Lucio Litti Andrea Colusso Marcella Pinto Erlis Ruli Alessia Scarsi Laura Ventura Giuseppe Toffoli Marco Colombatti Giulio Fracasso Moreno Meneghetti
author_facet	Lucio Litti Andrea Colusso Marcella Pinto Erlis Ruli Alessia Scarsi Laura Ventura Giuseppe Toffoli Marco Colombatti Giulio Fracasso Moreno Meneghetti
author_sort	Lucio Litti
title	SERRS multiplexing with multivalent nanostructures for the identification and enumeration of epithelial and mesenchymal cells
title_short	SERRS multiplexing with multivalent nanostructures for the identification and enumeration of epithelial and mesenchymal cells
title_full	SERRS multiplexing with multivalent nanostructures for the identification and enumeration of epithelial and mesenchymal cells
title_fullStr	SERRS multiplexing with multivalent nanostructures for the identification and enumeration of epithelial and mesenchymal cells
title_full_unstemmed	SERRS multiplexing with multivalent nanostructures for the identification and enumeration of epithelial and mesenchymal cells
title_sort	serrs multiplexing with multivalent nanostructures for the identification and enumeration of epithelial and mesenchymal cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/34aaa39c17f24ade84f6d894cc073d5f
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SERRS multiplexing with multivalent nanostructures for the identification and enumeration of epithelial and mesenchymal cells

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