Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine

Abstract Rhizopus oryzae (R. oryzae) can effectively produce organic acids, and its pellet formation in seed cultures has been shown to significantly enhance subsequent fermentation processes. Despite advances in strain development, simple and effective methods for inducing pellet morphology and a b...

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Autores principales:	Na Wu, Jiahui Zhang, Wen Ou, Yaru Chen, Ru Wang, Ke Li, Xiao-man Sun, Yingfeng Li, Qing Xu, He Huang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	R. oryzae Seed pellet formation Triethanolamine Organic acid Transcriptomic analysis Fuel TP315-360 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/34af404bdbc84d27bd5951a727deffad
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spelling	oai:doaj.org-article:34af404bdbc84d27bd5951a727deffad2021-12-05T12:16:43ZTranscriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine10.1186/s13068-021-02081-y1754-6834https://doaj.org/article/34af404bdbc84d27bd5951a727deffad2021-12-01T00:00:00Zhttps://doi.org/10.1186/s13068-021-02081-yhttps://doaj.org/toc/1754-6834Abstract Rhizopus oryzae (R. oryzae) can effectively produce organic acids, and its pellet formation in seed cultures has been shown to significantly enhance subsequent fermentation processes. Despite advances in strain development, simple and effective methods for inducing pellet morphology and a basic understanding of the mechanisms controlling this process could facilitate substantial increases in efficiency and product output. Here, we report that 1.5% triethanolamine (TEOA) in seed culture medium can activate the growth of R. oryzae spores in compact and uniform pellets which is optimal for fermentation conditions. Analysis of fermentation kinetics showed that the production of fumaric and L-malic acid increases 293% and 177%, respectively. Transcriptomic analysis revealed that exposure of R. oryzae to 1.5% TEOA during the seed culture activated the phosphatidylinositol and mitogen-activated protein kinase signaling pathways. Theses pathways subsequently stimulated the downstream carbohydrate-active synthases and hydrolases that required for cell wall component synthesis and reconstruction. Our results thus provide insight into the regulatory pathways controlling pellet morphology germane to the viability of seed cultures, and provide valuable reference data for subsequent optimization of organic acid fermentation by R. oryzae.Na WuJiahui ZhangWen OuYaru ChenRu WangKe LiXiao-man SunYingfeng LiQing XuHe HuangBMCarticleR. oryzaeSeed pellet formationTriethanolamineOrganic acidTranscriptomic analysisFuelTP315-360BiotechnologyTP248.13-248.65ENBiotechnology for Biofuels, Vol 14, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	R. oryzae Seed pellet formation Triethanolamine Organic acid Transcriptomic analysis Fuel TP315-360 Biotechnology TP248.13-248.65
spellingShingle	R. oryzae Seed pellet formation Triethanolamine Organic acid Transcriptomic analysis Fuel TP315-360 Biotechnology TP248.13-248.65 Na Wu Jiahui Zhang Wen Ou Yaru Chen Ru Wang Ke Li Xiao-man Sun Yingfeng Li Qing Xu He Huang Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine
description	Abstract Rhizopus oryzae (R. oryzae) can effectively produce organic acids, and its pellet formation in seed cultures has been shown to significantly enhance subsequent fermentation processes. Despite advances in strain development, simple and effective methods for inducing pellet morphology and a basic understanding of the mechanisms controlling this process could facilitate substantial increases in efficiency and product output. Here, we report that 1.5% triethanolamine (TEOA) in seed culture medium can activate the growth of R. oryzae spores in compact and uniform pellets which is optimal for fermentation conditions. Analysis of fermentation kinetics showed that the production of fumaric and L-malic acid increases 293% and 177%, respectively. Transcriptomic analysis revealed that exposure of R. oryzae to 1.5% TEOA during the seed culture activated the phosphatidylinositol and mitogen-activated protein kinase signaling pathways. Theses pathways subsequently stimulated the downstream carbohydrate-active synthases and hydrolases that required for cell wall component synthesis and reconstruction. Our results thus provide insight into the regulatory pathways controlling pellet morphology germane to the viability of seed cultures, and provide valuable reference data for subsequent optimization of organic acid fermentation by R. oryzae.
format	article
author	Na Wu Jiahui Zhang Wen Ou Yaru Chen Ru Wang Ke Li Xiao-man Sun Yingfeng Li Qing Xu He Huang
author_facet	Na Wu Jiahui Zhang Wen Ou Yaru Chen Ru Wang Ke Li Xiao-man Sun Yingfeng Li Qing Xu He Huang
author_sort	Na Wu
title	Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine
title_short	Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine
title_full	Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine
title_fullStr	Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine
title_full_unstemmed	Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine
title_sort	transcriptome analysis of rhizopus oryzae seed pellet formation using triethanolamine
publisher	BMC
publishDate	2021
url	https://doaj.org/article/34af404bdbc84d27bd5951a727deffad
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_version_	1718372067617800192

Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine

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