The Efficacy of AgNO3 Nanoparticles Alone and Conjugated with Imipenem for Combating Extensively Drug-Resistant Pseudomonas aeruginosa

Mahsa Shahbandeh,1 Majid Taati Moghadam,2,3 Reza Mirnejad,4 Shiva Mirkalantari,5 Mehrnaz Mirzaei6 1Young Researchers and Elite Club, Saveh Branch, Islamic Azad University, Saveh, Iran; 2Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran; 3Student Research Committee, Iran U...

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Autores principales:	Shahbandeh M, Taati Moghadam M, Mirnejad R, Mirkalantari S, Mirzaei M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	p. aeruginosa agno3 nanoparticle xdr imipenem Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/34b716786efc438c945b3e18c9a6b964
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spelling	oai:doaj.org-article:34b716786efc438c945b3e18c9a6b9642021-12-02T11:12:45ZThe Efficacy of AgNO3 Nanoparticles Alone and Conjugated with Imipenem for Combating Extensively Drug-Resistant Pseudomonas aeruginosa1178-2013https://doaj.org/article/34b716786efc438c945b3e18c9a6b9642020-09-01T00:00:00Zhttps://www.dovepress.com/the-efficacy-of-agno3-nanoparticles-alone-and-conjugated-with-imipenem-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mahsa Shahbandeh,1 Majid Taati Moghadam,2,3 Reza Mirnejad,4 Shiva Mirkalantari,5 Mehrnaz Mirzaei6 1Young Researchers and Elite Club, Saveh Branch, Islamic Azad University, Saveh, Iran; 2Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran; 3Student Research Committee, Iran University of Medical Sciences, Tehran, Iran; 4Molecular Biology Research Center, System Biology and Poisoning Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran; 5Microbiology, Faculty of Medicine, Microbiology Department, Iran University of Medical Sciences, Tehran, Iran; 6Department of Microbiology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, IranCorrespondence: : Reza MirnejadMolecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, IranEmail rmirnejad@bmsu.ac.irShiva MirkalantariMicrobiology, Faculty of Medicine, Microbiology Department, Iran University of Medical Sciences, Tehran, IranEmail Mirkalantari.sh@iums.ac.irIntroduction: The extensive drug-resistant (XDR) Pseudomonas aeruginosa (P. aeruginosa) causes a range of infections with high mortality rate, which inflicts additional costs on treatment. The use of nano-biotechnology-based methods in medicine has opened a new perspective against drug-resistant bacteria. The aim of this study was to evaluate the effectiveness of the AgNO3 nanoparticles alone and conjugated with imipenem (IMI) to combat extensively drug-resistant P. aeruginosa.Methods: Antibiotic susceptibility was carried out using disc diffusion method. Detection of different resistant genes was performed using standard polymerase chain reaction (PCR). The chemically synthesized AgNO3 particles were characterized using scanning electron microscope (SEM), dynamic light scattering (DLS) and X-ray diffraction (XRD) methods. Fourier transform infrared spectroscopy (FTIR) was accomplished to confirm the binding of AgNO3 with IMI. The microdilution broth method was used to obtain minimum inhibitory concentration (MIC) of AgNO3 and IMI-conjugated AgNO3. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was carried out on L929 cell line to study the cytotoxicity of nanoparticles. The data were analyzed by Eta correlation ratio and chi-square (X2) test.Results: Analysis of the antibiotic resistance pattern showed that 12 (24%) isolates were XDR, and MIC values of IMI were between 64 and 128 μg/mL. Frequency of SHV, TEM, CTX M, IMP, VIM, OPR, SIM, SPM, GIM, NDM, VEB, PER, KPC, OXA, intI, intII, and intIII genes were 29 (58%), 26 (52%), 26 (52%), 32 (64%), 23 (46%), 43 (86%), 3 (6%), 6 (12%), 3 (6%), 4 (8%), 7 (14%), 6 (12%), 18 (36%), 4 (8%), 19 (38%), 16 (32%), and 2 (4%), respectively. The XRD, SEM, DLS, and FTIR analysis confirmed the synthesis of AgNO3 nanoparticles and their conjugation with IMI. The AgNO3 nanoparticles had antimicrobial activity, and their conjugation with IMI showed enhanced effectiveness against XDR isolates. The synthesized AgNO3 showed no cytotoxic effects.Conclusion: The results suggest that IMI-conjugated AgNO3 has a strong potency as a powerful antibacterial agent against XDR P. aeruginosa.Keywords: P. aeruginosa, AgNO3 nanoparticle, XDR, imipenemShahbandeh MTaati Moghadam MMirnejad RMirkalantari SMirzaei MDove Medical Pressarticlep. aeruginosaagno3 nanoparticlexdrimipenemMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 6905-6916 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	p. aeruginosa agno3 nanoparticle xdr imipenem Medicine (General) R5-920
spellingShingle	p. aeruginosa agno3 nanoparticle xdr imipenem Medicine (General) R5-920 Shahbandeh M Taati Moghadam M Mirnejad R Mirkalantari S Mirzaei M The Efficacy of AgNO3 Nanoparticles Alone and Conjugated with Imipenem for Combating Extensively Drug-Resistant Pseudomonas aeruginosa
description	Mahsa Shahbandeh,1 Majid Taati Moghadam,2,3 Reza Mirnejad,4 Shiva Mirkalantari,5 Mehrnaz Mirzaei6 1Young Researchers and Elite Club, Saveh Branch, Islamic Azad University, Saveh, Iran; 2Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran; 3Student Research Committee, Iran University of Medical Sciences, Tehran, Iran; 4Molecular Biology Research Center, System Biology and Poisoning Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran; 5Microbiology, Faculty of Medicine, Microbiology Department, Iran University of Medical Sciences, Tehran, Iran; 6Department of Microbiology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, IranCorrespondence: : Reza MirnejadMolecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, IranEmail rmirnejad@bmsu.ac.irShiva MirkalantariMicrobiology, Faculty of Medicine, Microbiology Department, Iran University of Medical Sciences, Tehran, IranEmail Mirkalantari.sh@iums.ac.irIntroduction: The extensive drug-resistant (XDR) Pseudomonas aeruginosa (P. aeruginosa) causes a range of infections with high mortality rate, which inflicts additional costs on treatment. The use of nano-biotechnology-based methods in medicine has opened a new perspective against drug-resistant bacteria. The aim of this study was to evaluate the effectiveness of the AgNO3 nanoparticles alone and conjugated with imipenem (IMI) to combat extensively drug-resistant P. aeruginosa.Methods: Antibiotic susceptibility was carried out using disc diffusion method. Detection of different resistant genes was performed using standard polymerase chain reaction (PCR). The chemically synthesized AgNO3 particles were characterized using scanning electron microscope (SEM), dynamic light scattering (DLS) and X-ray diffraction (XRD) methods. Fourier transform infrared spectroscopy (FTIR) was accomplished to confirm the binding of AgNO3 with IMI. The microdilution broth method was used to obtain minimum inhibitory concentration (MIC) of AgNO3 and IMI-conjugated AgNO3. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was carried out on L929 cell line to study the cytotoxicity of nanoparticles. The data were analyzed by Eta correlation ratio and chi-square (X2) test.Results: Analysis of the antibiotic resistance pattern showed that 12 (24%) isolates were XDR, and MIC values of IMI were between 64 and 128 μg/mL. Frequency of SHV, TEM, CTX M, IMP, VIM, OPR, SIM, SPM, GIM, NDM, VEB, PER, KPC, OXA, intI, intII, and intIII genes were 29 (58%), 26 (52%), 26 (52%), 32 (64%), 23 (46%), 43 (86%), 3 (6%), 6 (12%), 3 (6%), 4 (8%), 7 (14%), 6 (12%), 18 (36%), 4 (8%), 19 (38%), 16 (32%), and 2 (4%), respectively. The XRD, SEM, DLS, and FTIR analysis confirmed the synthesis of AgNO3 nanoparticles and their conjugation with IMI. The AgNO3 nanoparticles had antimicrobial activity, and their conjugation with IMI showed enhanced effectiveness against XDR isolates. The synthesized AgNO3 showed no cytotoxic effects.Conclusion: The results suggest that IMI-conjugated AgNO3 has a strong potency as a powerful antibacterial agent against XDR P. aeruginosa.Keywords: P. aeruginosa, AgNO3 nanoparticle, XDR, imipenem
format	article
author	Shahbandeh M Taati Moghadam M Mirnejad R Mirkalantari S Mirzaei M
author_facet	Shahbandeh M Taati Moghadam M Mirnejad R Mirkalantari S Mirzaei M
author_sort	Shahbandeh M
title	The Efficacy of AgNO3 Nanoparticles Alone and Conjugated with Imipenem for Combating Extensively Drug-Resistant Pseudomonas aeruginosa
title_short	The Efficacy of AgNO3 Nanoparticles Alone and Conjugated with Imipenem for Combating Extensively Drug-Resistant Pseudomonas aeruginosa
title_full	The Efficacy of AgNO3 Nanoparticles Alone and Conjugated with Imipenem for Combating Extensively Drug-Resistant Pseudomonas aeruginosa
title_fullStr	The Efficacy of AgNO3 Nanoparticles Alone and Conjugated with Imipenem for Combating Extensively Drug-Resistant Pseudomonas aeruginosa
title_full_unstemmed	The Efficacy of AgNO3 Nanoparticles Alone and Conjugated with Imipenem for Combating Extensively Drug-Resistant Pseudomonas aeruginosa
title_sort	efficacy of agno3 nanoparticles alone and conjugated with imipenem for combating extensively drug-resistant pseudomonas aeruginosa
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/34b716786efc438c945b3e18c9a6b964
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The Efficacy of AgNO3 Nanoparticles Alone and Conjugated with Imipenem for Combating Extensively Drug-Resistant Pseudomonas aeruginosa

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