Integration of transcriptome and targeted metabolome profiling reveals hormone related genes involved in the growth of Bletilla striata

Abstract Bletilla striata (Thunb.) Reichb.f. (BS) is a traditional Chinese medicine with numerous beneficial effects. In our previous study, Aspergillus flavus was isolated from B. striata. To explore the physiological and molecular mechanisms of Aspergillus flavus elicitor (1-G4) that promoted Blet...

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Autores principales:	Hengwei Zou, Hanxiao Jiang, Liangbo Li, Rongshao Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/34c31aa9add34a438193ced43a23f13b
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spelling	oai:doaj.org-article:34c31aa9add34a438193ced43a23f13b2021-11-14T12:19:32ZIntegration of transcriptome and targeted metabolome profiling reveals hormone related genes involved in the growth of Bletilla striata10.1038/s41598-021-01532-82045-2322https://doaj.org/article/34c31aa9add34a438193ced43a23f13b2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01532-8https://doaj.org/toc/2045-2322Abstract Bletilla striata (Thunb.) Reichb.f. (BS) is a traditional Chinese medicine with numerous beneficial effects. In our previous study, Aspergillus flavus was isolated from B. striata. To explore the physiological and molecular mechanisms of Aspergillus flavus elicitor (1-G4) that promoted Bletilla striata growth, in this study, we performed the determination of growth indexes and transcriptomics and metabolomics analysis under 5% and 10% 1-G4 conditions. Results showed that 1-G4 elicitor could significantly promote the growth and development of B. striata. With the increasing concentration of 1-G4 elicitor, the contents of SA, ICAld, and ME-IAA significantly increased while the IP and ACC contents decreased dramatically. A total of 1657 DEGs (763 up-regulated and 894 down-regulated) between the control (CK) and 5% elicitor (CK vs G5) and 2415 DEGs (1208 up-regulated and 1207 down-regulated) between the control and 10% elicitor (CK vs G10) were identified. Further, we found that 22, 38, and 2 unigenes were involved in ME-IAA, IP, and ACC, respectively. It was indicated that these unigenes might be involved in B. striata growth. Overall, the current study laid a theoretical foundation for the effective utilization of endophytic fungi and the optimization of germplasm resources of B. striata.Hengwei ZouHanxiao JiangLiangbo LiRongshao HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hengwei Zou Hanxiao Jiang Liangbo Li Rongshao Huang Integration of transcriptome and targeted metabolome profiling reveals hormone related genes involved in the growth of Bletilla striata
description	Abstract Bletilla striata (Thunb.) Reichb.f. (BS) is a traditional Chinese medicine with numerous beneficial effects. In our previous study, Aspergillus flavus was isolated from B. striata. To explore the physiological and molecular mechanisms of Aspergillus flavus elicitor (1-G4) that promoted Bletilla striata growth, in this study, we performed the determination of growth indexes and transcriptomics and metabolomics analysis under 5% and 10% 1-G4 conditions. Results showed that 1-G4 elicitor could significantly promote the growth and development of B. striata. With the increasing concentration of 1-G4 elicitor, the contents of SA, ICAld, and ME-IAA significantly increased while the IP and ACC contents decreased dramatically. A total of 1657 DEGs (763 up-regulated and 894 down-regulated) between the control (CK) and 5% elicitor (CK vs G5) and 2415 DEGs (1208 up-regulated and 1207 down-regulated) between the control and 10% elicitor (CK vs G10) were identified. Further, we found that 22, 38, and 2 unigenes were involved in ME-IAA, IP, and ACC, respectively. It was indicated that these unigenes might be involved in B. striata growth. Overall, the current study laid a theoretical foundation for the effective utilization of endophytic fungi and the optimization of germplasm resources of B. striata.
format	article
author	Hengwei Zou Hanxiao Jiang Liangbo Li Rongshao Huang
author_facet	Hengwei Zou Hanxiao Jiang Liangbo Li Rongshao Huang
author_sort	Hengwei Zou
title	Integration of transcriptome and targeted metabolome profiling reveals hormone related genes involved in the growth of Bletilla striata
title_short	Integration of transcriptome and targeted metabolome profiling reveals hormone related genes involved in the growth of Bletilla striata
title_full	Integration of transcriptome and targeted metabolome profiling reveals hormone related genes involved in the growth of Bletilla striata
title_fullStr	Integration of transcriptome and targeted metabolome profiling reveals hormone related genes involved in the growth of Bletilla striata
title_full_unstemmed	Integration of transcriptome and targeted metabolome profiling reveals hormone related genes involved in the growth of Bletilla striata
title_sort	integration of transcriptome and targeted metabolome profiling reveals hormone related genes involved in the growth of bletilla striata
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/34c31aa9add34a438193ced43a23f13b
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Integration of transcriptome and targeted metabolome profiling reveals hormone related genes involved in the growth of Bletilla striata

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