Photodynamic therapy with the dual-mode association of IR780 to PEG-PLA nanocapsules and the effects on human breast cancer cells

IR780 is a near-infrared fluorescent dye, which can be applied as a photosensitizer in photodynamic (PDT) and photothermal (PTT) therapies and as a biodistribution tracer in imaging techniques. We investigated the growth and migration inhibition and mechanism of death of breast tumor cells, MCF-7 an...

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Autores principales:	Marina Guimarães Carvalho Machado, Maria Alice de Oliveira, Elisa Gomes Lanna, Raoni Pais Siqueira, Gwenaelle Pound-Lana, Renata Tupinambá Branquinho, Vanessa Carla Furtado Mosqueira
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Phototheranostics IR780 Polymeric nanocapsules Image-guided therapy Photodynamic therapy Polymer conjugation Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/34cc528a417d4fe3950c745309b8b63e
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spelling	oai:doaj.org-article:34cc528a417d4fe3950c745309b8b63e2021-12-02T04:59:07ZPhotodynamic therapy with the dual-mode association of IR780 to PEG-PLA nanocapsules and the effects on human breast cancer cells0753-332210.1016/j.biopha.2021.112464https://doaj.org/article/34cc528a417d4fe3950c745309b8b63e2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221012506https://doaj.org/toc/0753-3322IR780 is a near-infrared fluorescent dye, which can be applied as a photosensitizer in photodynamic (PDT) and photothermal (PTT) therapies and as a biodistribution tracer in imaging techniques. We investigated the growth and migration inhibition and mechanism of death of breast tumor cells, MCF-7 and MDA-MB-231, exposed to polymeric nanocapsules (NC) comprising IR780 covalently linked to the biodegradable polymer PLA (IR-PLA) and IR780 physically encapsulated (IR780-NC) in vitro. Both types of NC had mean diameters around 120 nm and zeta potentials around −40 mV. IR-PLA-NC was less cytotoxic than IR780 NC to a non-tumorigenic mammary epithelial cell line, MCF-10A, which is an important aspect of selectivity. Free-IR780 was more cytotoxic than IR-PLA-NC for MCF-7 and MDA-MB-231 cells after illumination with a 808 nm laser. IR-PLA NC was effective to inhibit colony formation (50%) and migration (30–40%) for both cancer cell lines. MDA-MB-231 cells were less sensitive to all IR780 formulations compared to MCF-7 cells. Cell uptake was higher with IR-PLA-NC than with IR780-NC and free-IR780 in both cancer cell lines (p < 0.05). NC uptake was higher in MCF-7 than in MDA-MB-231 cells. IR-PLA-NC induced a higher percentage of apoptosis upon illumination in MDA-MB-231 than in MCF-7 cells. The necrosis mechanism of death predominated in treatments with free-IR780 and with encapsulated IR780 NC, suggestive of damages at the plasma membrane. IR780 conjugated with PLA increased the apoptotic pathway and demonstrated potential as a multifunctional theranostic agent for breast cancer treatment with increased cellular uptake, photodynamic activity and more reliable tracking in cell-image studies.Marina Guimarães Carvalho MachadoMaria Alice de OliveiraElisa Gomes LannaRaoni Pais SiqueiraGwenaelle Pound-LanaRenata Tupinambá BranquinhoVanessa Carla Furtado MosqueiraElsevierarticlePhototheranosticsIR780Polymeric nanocapsulesImage-guided therapyPhotodynamic therapyPolymer conjugationTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112464- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Phototheranostics IR780 Polymeric nanocapsules Image-guided therapy Photodynamic therapy Polymer conjugation Therapeutics. Pharmacology RM1-950
spellingShingle	Phototheranostics IR780 Polymeric nanocapsules Image-guided therapy Photodynamic therapy Polymer conjugation Therapeutics. Pharmacology RM1-950 Marina Guimarães Carvalho Machado Maria Alice de Oliveira Elisa Gomes Lanna Raoni Pais Siqueira Gwenaelle Pound-Lana Renata Tupinambá Branquinho Vanessa Carla Furtado Mosqueira Photodynamic therapy with the dual-mode association of IR780 to PEG-PLA nanocapsules and the effects on human breast cancer cells
description	IR780 is a near-infrared fluorescent dye, which can be applied as a photosensitizer in photodynamic (PDT) and photothermal (PTT) therapies and as a biodistribution tracer in imaging techniques. We investigated the growth and migration inhibition and mechanism of death of breast tumor cells, MCF-7 and MDA-MB-231, exposed to polymeric nanocapsules (NC) comprising IR780 covalently linked to the biodegradable polymer PLA (IR-PLA) and IR780 physically encapsulated (IR780-NC) in vitro. Both types of NC had mean diameters around 120 nm and zeta potentials around −40 mV. IR-PLA-NC was less cytotoxic than IR780 NC to a non-tumorigenic mammary epithelial cell line, MCF-10A, which is an important aspect of selectivity. Free-IR780 was more cytotoxic than IR-PLA-NC for MCF-7 and MDA-MB-231 cells after illumination with a 808 nm laser. IR-PLA NC was effective to inhibit colony formation (50%) and migration (30–40%) for both cancer cell lines. MDA-MB-231 cells were less sensitive to all IR780 formulations compared to MCF-7 cells. Cell uptake was higher with IR-PLA-NC than with IR780-NC and free-IR780 in both cancer cell lines (p < 0.05). NC uptake was higher in MCF-7 than in MDA-MB-231 cells. IR-PLA-NC induced a higher percentage of apoptosis upon illumination in MDA-MB-231 than in MCF-7 cells. The necrosis mechanism of death predominated in treatments with free-IR780 and with encapsulated IR780 NC, suggestive of damages at the plasma membrane. IR780 conjugated with PLA increased the apoptotic pathway and demonstrated potential as a multifunctional theranostic agent for breast cancer treatment with increased cellular uptake, photodynamic activity and more reliable tracking in cell-image studies.
format	article
author	Marina Guimarães Carvalho Machado Maria Alice de Oliveira Elisa Gomes Lanna Raoni Pais Siqueira Gwenaelle Pound-Lana Renata Tupinambá Branquinho Vanessa Carla Furtado Mosqueira
author_facet	Marina Guimarães Carvalho Machado Maria Alice de Oliveira Elisa Gomes Lanna Raoni Pais Siqueira Gwenaelle Pound-Lana Renata Tupinambá Branquinho Vanessa Carla Furtado Mosqueira
author_sort	Marina Guimarães Carvalho Machado
title	Photodynamic therapy with the dual-mode association of IR780 to PEG-PLA nanocapsules and the effects on human breast cancer cells
title_short	Photodynamic therapy with the dual-mode association of IR780 to PEG-PLA nanocapsules and the effects on human breast cancer cells
title_full	Photodynamic therapy with the dual-mode association of IR780 to PEG-PLA nanocapsules and the effects on human breast cancer cells
title_fullStr	Photodynamic therapy with the dual-mode association of IR780 to PEG-PLA nanocapsules and the effects on human breast cancer cells
title_full_unstemmed	Photodynamic therapy with the dual-mode association of IR780 to PEG-PLA nanocapsules and the effects on human breast cancer cells
title_sort	photodynamic therapy with the dual-mode association of ir780 to peg-pla nanocapsules and the effects on human breast cancer cells
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/34cc528a417d4fe3950c745309b8b63e
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Photodynamic therapy with the dual-mode association of IR780 to PEG-PLA nanocapsules and the effects on human breast cancer cells

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