Bitter Taste Receptors Expression in Human Granulosa and Cumulus Cells: New Perspectives in Female Fertility

Bitter taste receptors (TAS2RS) expression is not restricted to the oral cavity and the presence of these receptors in the male reproductive system and sperm provides insights into their possible role in human reproduction. To elucidate the potential role of TAS2Rs in the female reproductive system,...

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Autores principales:	Bianca Semplici, Francesca Paola Luongo, Sofia Passaponti, Claudia Landi, Laura Governini, Giuseppe Morgante, Vincenzo De Leo, Paola Piomboni, Alice Luddi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bitter taste receptors cumulus cells granulosa cells female fertility oocyte quality Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/34cd38cbd16f48cba2117dca76739fd7
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spelling	oai:doaj.org-article:34cd38cbd16f48cba2117dca76739fd72021-11-25T17:11:47ZBitter Taste Receptors Expression in Human Granulosa and Cumulus Cells: New Perspectives in Female Fertility10.3390/cells101131272073-4409https://doaj.org/article/34cd38cbd16f48cba2117dca76739fd72021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3127https://doaj.org/toc/2073-4409Bitter taste receptors (TAS2RS) expression is not restricted to the oral cavity and the presence of these receptors in the male reproductive system and sperm provides insights into their possible role in human reproduction. To elucidate the potential role of TAS2Rs in the female reproductive system, we investigated the expression and localization of bitter taste receptors and the components of signal transduction cascade involved in the pathway of taste receptors in somatic follicular cells obtained from women undergoing assisted reproductive techniques. We found that TAS2R genes are expressed in both cumulus (CCs) and granulosa (GCs) cells, with TAS2R14 being the most highly expressed bitter receptor subtype. Interestingly, a slight increase in the expression of TAS2R14 and TAS2R43 was shown in both GCs and CCs in young women (<i>p</i> < 0.05), while a negative correlation may be established between the number of oocytes collected at the pickup and the expression of TAS2R43. Regarding α-gustducin and α-transducin, two Gα subunits expressed in the taste buds on the tongue, we provide evidence for their expression in CCs and GCs, with α-gustducin showing two additional isoforms in GCs. Finally, we shed light on the possible downstream transduction pathway initiated by taste receptor activation in the female reproductive system. Our study, showing for the first time the expression of taste receptors in the somatic ovarian follicle cells, significantly extends the current knowledge of the biological role of TAS2Rs for human female fertility.Bianca SempliciFrancesca Paola LuongoSofia PassapontiClaudia LandiLaura GoverniniGiuseppe MorganteVincenzo De LeoPaola PiomboniAlice LuddiMDPI AGarticlebitter taste receptorscumulus cellsgranulosa cellsfemale fertilityoocyte qualityBiology (General)QH301-705.5ENCells, Vol 10, Iss 3127, p 3127 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bitter taste receptors cumulus cells granulosa cells female fertility oocyte quality Biology (General) QH301-705.5
spellingShingle	bitter taste receptors cumulus cells granulosa cells female fertility oocyte quality Biology (General) QH301-705.5 Bianca Semplici Francesca Paola Luongo Sofia Passaponti Claudia Landi Laura Governini Giuseppe Morgante Vincenzo De Leo Paola Piomboni Alice Luddi Bitter Taste Receptors Expression in Human Granulosa and Cumulus Cells: New Perspectives in Female Fertility
description	Bitter taste receptors (TAS2RS) expression is not restricted to the oral cavity and the presence of these receptors in the male reproductive system and sperm provides insights into their possible role in human reproduction. To elucidate the potential role of TAS2Rs in the female reproductive system, we investigated the expression and localization of bitter taste receptors and the components of signal transduction cascade involved in the pathway of taste receptors in somatic follicular cells obtained from women undergoing assisted reproductive techniques. We found that TAS2R genes are expressed in both cumulus (CCs) and granulosa (GCs) cells, with TAS2R14 being the most highly expressed bitter receptor subtype. Interestingly, a slight increase in the expression of TAS2R14 and TAS2R43 was shown in both GCs and CCs in young women (<i>p</i> < 0.05), while a negative correlation may be established between the number of oocytes collected at the pickup and the expression of TAS2R43. Regarding α-gustducin and α-transducin, two Gα subunits expressed in the taste buds on the tongue, we provide evidence for their expression in CCs and GCs, with α-gustducin showing two additional isoforms in GCs. Finally, we shed light on the possible downstream transduction pathway initiated by taste receptor activation in the female reproductive system. Our study, showing for the first time the expression of taste receptors in the somatic ovarian follicle cells, significantly extends the current knowledge of the biological role of TAS2Rs for human female fertility.
format	article
author	Bianca Semplici Francesca Paola Luongo Sofia Passaponti Claudia Landi Laura Governini Giuseppe Morgante Vincenzo De Leo Paola Piomboni Alice Luddi
author_facet	Bianca Semplici Francesca Paola Luongo Sofia Passaponti Claudia Landi Laura Governini Giuseppe Morgante Vincenzo De Leo Paola Piomboni Alice Luddi
author_sort	Bianca Semplici
title	Bitter Taste Receptors Expression in Human Granulosa and Cumulus Cells: New Perspectives in Female Fertility
title_short	Bitter Taste Receptors Expression in Human Granulosa and Cumulus Cells: New Perspectives in Female Fertility
title_full	Bitter Taste Receptors Expression in Human Granulosa and Cumulus Cells: New Perspectives in Female Fertility
title_fullStr	Bitter Taste Receptors Expression in Human Granulosa and Cumulus Cells: New Perspectives in Female Fertility
title_full_unstemmed	Bitter Taste Receptors Expression in Human Granulosa and Cumulus Cells: New Perspectives in Female Fertility
title_sort	bitter taste receptors expression in human granulosa and cumulus cells: new perspectives in female fertility
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/34cd38cbd16f48cba2117dca76739fd7
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Bitter Taste Receptors Expression in Human Granulosa and Cumulus Cells: New Perspectives in Female Fertility

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