Glucocorticoids induce osteonecrosis of the femoral head through the Hippo signaling pathway

Osteonecrosis of the femoral head (ONFH) induced by glucocorticoids (GCs) has been considered to be associated with the dysfunction of bone marrow mesenchymal stem cells (BMSCs). Studies have reported that GCs can regulate the normal differentiation of BMSCs. However, the exact mechanism of this reg...

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Autores principales:	Li Yugang, Xu Zechuan, Chang Shan
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	bmsc gc onfh hippo signaling pathway Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/34d315ca938743cca260c5ea021fb562
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spelling	oai:doaj.org-article:34d315ca938743cca260c5ea021fb5622021-12-05T14:10:41ZGlucocorticoids induce osteonecrosis of the femoral head through the Hippo signaling pathway2391-541210.1515/biol-2021-0102https://doaj.org/article/34d315ca938743cca260c5ea021fb5622021-10-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0102https://doaj.org/toc/2391-5412Osteonecrosis of the femoral head (ONFH) induced by glucocorticoids (GCs) has been considered to be associated with the dysfunction of bone marrow mesenchymal stem cells (BMSCs). Studies have reported that GCs can regulate the normal differentiation of BMSCs. However, the exact mechanism of this regulation remains unclear. In this study, we used methylprednisolone (MPS) to induce BMSCs, and then found that the Hippo signaling pathway was upregulated in a dose-dependent manner compared to that in the control group. In addition, the osteogenic ability of BMSCs was decreased, as evaluated by Alizarin Red S staining analysis and alkaline phosphatase activity assays, accompanied by the downregulated expression of Runx2, osteopontin, and osteocalcin. Additionally, the adipogenic capacity of BMSCs under the MPS conditions was increased, as identified by Oil Red O staining with upregulated triglyceride and PPARγ expression. Moreover, suppression by knockdown of MST1 was found to attenuate the Hippo signaling pathway and adipogenic differentiation, while enhancing osteogenic differentiation. In conclusion, our findings revealed that the Hippo signaling pathway was involved in GC-ONFH by affecting the osteogenic and adipogenic differentiation capacities of BMSCs. Our study could provide a basis for further investigation of the specific function of the Hippo pathway in ONFH.Li YugangXu ZechuanChang ShanDe Gruyterarticlebmscgconfhhippo signaling pathwayBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 1130-1140 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bmsc gc onfh hippo signaling pathway Biology (General) QH301-705.5
spellingShingle	bmsc gc onfh hippo signaling pathway Biology (General) QH301-705.5 Li Yugang Xu Zechuan Chang Shan Glucocorticoids induce osteonecrosis of the femoral head through the Hippo signaling pathway
description	Osteonecrosis of the femoral head (ONFH) induced by glucocorticoids (GCs) has been considered to be associated with the dysfunction of bone marrow mesenchymal stem cells (BMSCs). Studies have reported that GCs can regulate the normal differentiation of BMSCs. However, the exact mechanism of this regulation remains unclear. In this study, we used methylprednisolone (MPS) to induce BMSCs, and then found that the Hippo signaling pathway was upregulated in a dose-dependent manner compared to that in the control group. In addition, the osteogenic ability of BMSCs was decreased, as evaluated by Alizarin Red S staining analysis and alkaline phosphatase activity assays, accompanied by the downregulated expression of Runx2, osteopontin, and osteocalcin. Additionally, the adipogenic capacity of BMSCs under the MPS conditions was increased, as identified by Oil Red O staining with upregulated triglyceride and PPARγ expression. Moreover, suppression by knockdown of MST1 was found to attenuate the Hippo signaling pathway and adipogenic differentiation, while enhancing osteogenic differentiation. In conclusion, our findings revealed that the Hippo signaling pathway was involved in GC-ONFH by affecting the osteogenic and adipogenic differentiation capacities of BMSCs. Our study could provide a basis for further investigation of the specific function of the Hippo pathway in ONFH.
format	article
author	Li Yugang Xu Zechuan Chang Shan
author_facet	Li Yugang Xu Zechuan Chang Shan
author_sort	Li Yugang
title	Glucocorticoids induce osteonecrosis of the femoral head through the Hippo signaling pathway
title_short	Glucocorticoids induce osteonecrosis of the femoral head through the Hippo signaling pathway
title_full	Glucocorticoids induce osteonecrosis of the femoral head through the Hippo signaling pathway
title_fullStr	Glucocorticoids induce osteonecrosis of the femoral head through the Hippo signaling pathway
title_full_unstemmed	Glucocorticoids induce osteonecrosis of the femoral head through the Hippo signaling pathway
title_sort	glucocorticoids induce osteonecrosis of the femoral head through the hippo signaling pathway
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/34d315ca938743cca260c5ea021fb562
work_keys_str_mv	AT liyugang glucocorticoidsinduceosteonecrosisofthefemoralheadthroughthehipposignalingpathway AT xuzechuan glucocorticoidsinduceosteonecrosisofthefemoralheadthroughthehipposignalingpathway AT changshan glucocorticoidsinduceosteonecrosisofthefemoralheadthroughthehipposignalingpathway
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Glucocorticoids induce osteonecrosis of the femoral head through the Hippo signaling pathway

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