Preparation and evaluation of the cytotoxic nature of TiO2 nanoparticles by direct contact method

M Chellappa,1 U Anjaneyulu,1 Geetha Manivasagam,2 U Vijayalakshmi1 1School of Advanced Sciences, Materials Chemistry Division, 2Centre for Biomaterials Science and Technology, School of Mechanical and Building Sciences, VIT University, Vellore, Tamil Nadu, India Abstract: The purpose of t...

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Autores principales:	Chellappa M, Anjaneyulu U, Manivasagam G, Vijayalakshmi U
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/34d3b19e8419460c9dcfe0b3b130bcd6
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spelling	oai:doaj.org-article:34d3b19e8419460c9dcfe0b3b130bcd62021-12-02T03:05:17ZPreparation and evaluation of the cytotoxic nature of TiO2 nanoparticles by direct contact method1178-2013https://doaj.org/article/34d3b19e8419460c9dcfe0b3b130bcd62015-10-01T00:00:00Zhttps://www.dovepress.com/preparation-and-evaluation-of-the-cytotoxic-nature-of-tio2-nanoparticl-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013M Chellappa,1 U Anjaneyulu,1 Geetha Manivasagam,2 U Vijayalakshmi1 1School of Advanced Sciences, Materials Chemistry Division, 2Centre for Biomaterials Science and Technology, School of Mechanical and Building Sciences, VIT University, Vellore, Tamil Nadu, India Abstract: The purpose of this study is to prepare and evaluate the effect of synthesized titanium dioxide (TiO2) nanoparticles for their biocompatibility on physiological body fluids and the effect of cell toxicity to produce osteointegration when used as implantable materials. For the past few decades, the number of researches done to understand the importance of the biocompatibility of bioceramics, metals, and polymers and their effect on clinical settings of biomedical devices has increased. Hence, the total concept of biocompatibility encourages researchers to actively engage in the investigation of the most compatible materials in living systems by analyzing them using suitable physical, chemical, and biological (bioassay) methods. The ceramic material nano TiO2 was prepared by sol-gel method and analyzed for its functional group and phase formation by Fourier transform infrared spectroscopy and powder X-ray diffraction. Furthermore, the particle size, shape, surface topography, and morphological behavior were analyzed by dynamic light scattering, zeta potential, scanning electron microscopy–energy dispersive X-ray analysis, and transmission electron microscopy analysis. In addition to this, the cytotoxicity and cytocompatibility were determined on MG63 cell lines with varying doses of concentrations such as 1 µg/mL, 10 µg/mL, 25 µg/mL, 50 µg/mL, and 100 µg/mL with different time periods such as 24 hours and 48 hours. The results have not shown any toxicity, whereas, it improved the cell viability/proliferation at various concentrations. Hence, these findings indicate that the nano TiO2 material acts as a good implantable material when used in the biomedical field as a prime surface-modifying agent. Keywords: biomaterial, implant, biocompatible, MTT assay, osteointegration, MG63 cell linesChellappa MAnjaneyulu UManivasagam GVijayalakshmi UDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss Supplement 1 Challenges in biomaterials research, Pp 31-41 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Chellappa M Anjaneyulu U Manivasagam G Vijayalakshmi U Preparation and evaluation of the cytotoxic nature of TiO2 nanoparticles by direct contact method
description	M Chellappa,1 U Anjaneyulu,1 Geetha Manivasagam,2 U Vijayalakshmi1 1School of Advanced Sciences, Materials Chemistry Division, 2Centre for Biomaterials Science and Technology, School of Mechanical and Building Sciences, VIT University, Vellore, Tamil Nadu, India Abstract: The purpose of this study is to prepare and evaluate the effect of synthesized titanium dioxide (TiO2) nanoparticles for their biocompatibility on physiological body fluids and the effect of cell toxicity to produce osteointegration when used as implantable materials. For the past few decades, the number of researches done to understand the importance of the biocompatibility of bioceramics, metals, and polymers and their effect on clinical settings of biomedical devices has increased. Hence, the total concept of biocompatibility encourages researchers to actively engage in the investigation of the most compatible materials in living systems by analyzing them using suitable physical, chemical, and biological (bioassay) methods. The ceramic material nano TiO2 was prepared by sol-gel method and analyzed for its functional group and phase formation by Fourier transform infrared spectroscopy and powder X-ray diffraction. Furthermore, the particle size, shape, surface topography, and morphological behavior were analyzed by dynamic light scattering, zeta potential, scanning electron microscopy–energy dispersive X-ray analysis, and transmission electron microscopy analysis. In addition to this, the cytotoxicity and cytocompatibility were determined on MG63 cell lines with varying doses of concentrations such as 1 µg/mL, 10 µg/mL, 25 µg/mL, 50 µg/mL, and 100 µg/mL with different time periods such as 24 hours and 48 hours. The results have not shown any toxicity, whereas, it improved the cell viability/proliferation at various concentrations. Hence, these findings indicate that the nano TiO2 material acts as a good implantable material when used in the biomedical field as a prime surface-modifying agent. Keywords: biomaterial, implant, biocompatible, MTT assay, osteointegration, MG63 cell lines
format	article
author	Chellappa M Anjaneyulu U Manivasagam G Vijayalakshmi U
author_facet	Chellappa M Anjaneyulu U Manivasagam G Vijayalakshmi U
author_sort	Chellappa M
title	Preparation and evaluation of the cytotoxic nature of TiO2 nanoparticles by direct contact method
title_short	Preparation and evaluation of the cytotoxic nature of TiO2 nanoparticles by direct contact method
title_full	Preparation and evaluation of the cytotoxic nature of TiO2 nanoparticles by direct contact method
title_fullStr	Preparation and evaluation of the cytotoxic nature of TiO2 nanoparticles by direct contact method
title_full_unstemmed	Preparation and evaluation of the cytotoxic nature of TiO2 nanoparticles by direct contact method
title_sort	preparation and evaluation of the cytotoxic nature of tio2 nanoparticles by direct contact method
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/34d3b19e8419460c9dcfe0b3b130bcd6
work_keys_str_mv	AT chellappam preparationandevaluationofthecytotoxicnatureoftio2nanoparticlesbydirectcontactmethod AT anjaneyuluu preparationandevaluationofthecytotoxicnatureoftio2nanoparticlesbydirectcontactmethod AT manivasagamg preparationandevaluationofthecytotoxicnatureoftio2nanoparticlesbydirectcontactmethod AT vijayalakshmiu preparationandevaluationofthecytotoxicnatureoftio2nanoparticlesbydirectcontactmethod
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Preparation and evaluation of the cytotoxic nature of TiO2 nanoparticles by direct contact method

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