Quantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism

Souabni et al. introduce a new analytical method for the study of ABC tripartite efflux pumps, by using a lipid scaffold that mimics the protein’s native environment and a spectroscopic assay, employing quantum dots (QD), that monitors ATP hydrolysis and substrate transport in real-time. When applie...

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Autores principales:	Hager Souabni, William Batista dos Santos, Quentin Cece, Laurent J. Catoire, Dhenesh Puvanendran, Vassiliy N. Bavro, Martin Picard
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/34d57d08da4545708d6340d7dc69c125
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spelling	oai:doaj.org-article:34d57d08da4545708d6340d7dc69c1252021-12-02T13:44:14ZQuantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism10.1038/s42003-021-01997-32399-3642https://doaj.org/article/34d57d08da4545708d6340d7dc69c1252021-04-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01997-3https://doaj.org/toc/2399-3642Souabni et al. introduce a new analytical method for the study of ABC tripartite efflux pumps, by using a lipid scaffold that mimics the protein’s native environment and a spectroscopic assay, employing quantum dots (QD), that monitors ATP hydrolysis and substrate transport in real-time. When applied to MacAB-TolC tripartite pump complex, results suggest high coupling efficiency between transport and energy consumption in the system.Hager SouabniWilliam Batista dos SantosQuentin CeceLaurent J. CatoireDhenesh PuvanendranVassiliy N. BavroMartin PicardNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Hager Souabni William Batista dos Santos Quentin Cece Laurent J. Catoire Dhenesh Puvanendran Vassiliy N. Bavro Martin Picard Quantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism
description	Souabni et al. introduce a new analytical method for the study of ABC tripartite efflux pumps, by using a lipid scaffold that mimics the protein’s native environment and a spectroscopic assay, employing quantum dots (QD), that monitors ATP hydrolysis and substrate transport in real-time. When applied to MacAB-TolC tripartite pump complex, results suggest high coupling efficiency between transport and energy consumption in the system.
format	article
author	Hager Souabni William Batista dos Santos Quentin Cece Laurent J. Catoire Dhenesh Puvanendran Vassiliy N. Bavro Martin Picard
author_facet	Hager Souabni William Batista dos Santos Quentin Cece Laurent J. Catoire Dhenesh Puvanendran Vassiliy N. Bavro Martin Picard
author_sort	Hager Souabni
title	Quantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism
title_short	Quantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism
title_full	Quantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism
title_fullStr	Quantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism
title_full_unstemmed	Quantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism
title_sort	quantitative real-time analysis of the efflux by the macab-tolc tripartite efflux pump clarifies the role of atp hydrolysis within mechanotransmission mechanism
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/34d57d08da4545708d6340d7dc69c125
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Quantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism

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