Quantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism
Souabni et al. introduce a new analytical method for the study of ABC tripartite efflux pumps, by using a lipid scaffold that mimics the protein’s native environment and a spectroscopic assay, employing quantum dots (QD), that monitors ATP hydrolysis and substrate transport in real-time. When applie...
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| Main Authors: | , , , , , , |
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| Format: | article |
| Language: | EN |
| Published: |
Nature Portfolio
2021
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| Subjects: | |
| Online Access: | https://doaj.org/article/34d57d08da4545708d6340d7dc69c125 |
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