Engineering Approaches in Plant Molecular Farming for Global Health

Since the demonstration of the first plant-produced proteins of medical interest, there has been significant growth and interest in the field of plant molecular farming, with plants now being considered a viable production platform for vaccines. Despite this interest and development by a few biophar...

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Autores principales:	Advaita Acarya Singh, Priyen Pillay, Tsepo Lebiletsa Tsekoa
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	plant-made pharmaceutical molecular farming vaccine glycoengineering tyrosine sulfation proteases inactivation Medicine R
Acceso en línea:	https://doaj.org/article/34db2a8db141455c95644fe640a01266
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spelling	oai:doaj.org-article:34db2a8db141455c95644fe640a012662021-11-25T19:10:48ZEngineering Approaches in Plant Molecular Farming for Global Health10.3390/vaccines91112702076-393Xhttps://doaj.org/article/34db2a8db141455c95644fe640a012662021-11-01T00:00:00Zhttps://www.mdpi.com/2076-393X/9/11/1270https://doaj.org/toc/2076-393XSince the demonstration of the first plant-produced proteins of medical interest, there has been significant growth and interest in the field of plant molecular farming, with plants now being considered a viable production platform for vaccines. Despite this interest and development by a few biopharmaceutical companies, plant molecular farming is yet to be embraced by ‘big pharma’. The plant system offers a faster alternative, which is a potentially more cost-effective and scalable platform for the mass production of highly complex protein vaccines, owing to the high degree of similarity between the plant and mammalian secretory pathway. Here, we identify and address bottlenecks in the use of plants for vaccine manufacturing and discuss engineering approaches that demonstrate both the utility and versatility of the plant production system as a viable biomanufacturing platform for global health. Strategies for improving the yields and quality of plant-produced vaccines, as well as the incorporation of authentic posttranslational modifications that are essential to the functionality of these highly complex protein vaccines, will also be discussed. Case-by-case examples are considered for improving the production of functional protein-based vaccines. The combination of all these strategies provides a basis for the use of cutting-edge genome editing technology to create a general plant chassis with reduced host cell proteins, which is optimised for high-level protein production of vaccines with the correct posttranslational modifications.Advaita Acarya SinghPriyen PillayTsepo Lebiletsa TsekoaMDPI AGarticleplant-made pharmaceuticalmolecular farmingvaccineglycoengineeringtyrosine sulfationproteases inactivationMedicineRENVaccines, Vol 9, Iss 1270, p 1270 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plant-made pharmaceutical molecular farming vaccine glycoengineering tyrosine sulfation proteases inactivation Medicine R
spellingShingle	plant-made pharmaceutical molecular farming vaccine glycoengineering tyrosine sulfation proteases inactivation Medicine R Advaita Acarya Singh Priyen Pillay Tsepo Lebiletsa Tsekoa Engineering Approaches in Plant Molecular Farming for Global Health
description	Since the demonstration of the first plant-produced proteins of medical interest, there has been significant growth and interest in the field of plant molecular farming, with plants now being considered a viable production platform for vaccines. Despite this interest and development by a few biopharmaceutical companies, plant molecular farming is yet to be embraced by ‘big pharma’. The plant system offers a faster alternative, which is a potentially more cost-effective and scalable platform for the mass production of highly complex protein vaccines, owing to the high degree of similarity between the plant and mammalian secretory pathway. Here, we identify and address bottlenecks in the use of plants for vaccine manufacturing and discuss engineering approaches that demonstrate both the utility and versatility of the plant production system as a viable biomanufacturing platform for global health. Strategies for improving the yields and quality of plant-produced vaccines, as well as the incorporation of authentic posttranslational modifications that are essential to the functionality of these highly complex protein vaccines, will also be discussed. Case-by-case examples are considered for improving the production of functional protein-based vaccines. The combination of all these strategies provides a basis for the use of cutting-edge genome editing technology to create a general plant chassis with reduced host cell proteins, which is optimised for high-level protein production of vaccines with the correct posttranslational modifications.
format	article
author	Advaita Acarya Singh Priyen Pillay Tsepo Lebiletsa Tsekoa
author_facet	Advaita Acarya Singh Priyen Pillay Tsepo Lebiletsa Tsekoa
author_sort	Advaita Acarya Singh
title	Engineering Approaches in Plant Molecular Farming for Global Health
title_short	Engineering Approaches in Plant Molecular Farming for Global Health
title_full	Engineering Approaches in Plant Molecular Farming for Global Health
title_fullStr	Engineering Approaches in Plant Molecular Farming for Global Health
title_full_unstemmed	Engineering Approaches in Plant Molecular Farming for Global Health
title_sort	engineering approaches in plant molecular farming for global health
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/34db2a8db141455c95644fe640a01266
work_keys_str_mv	AT advaitaacaryasingh engineeringapproachesinplantmolecularfarmingforglobalhealth AT priyenpillay engineeringapproachesinplantmolecularfarmingforglobalhealth AT tsepolebiletsatsekoa engineeringapproachesinplantmolecularfarmingforglobalhealth
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Engineering Approaches in Plant Molecular Farming for Global Health

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