RNA-Seq of early-infected poplar leaves by the rust pathogen Melampsora larici-populina uncovers PtSultr3;5, a fungal-induced host sulfate transporter.

Biotroph pathogens establish intimate interactions with their hosts that are conditioned by the successful secretion of effectors in infected tissues and subsequent manipulation of host physiology. The identification of early-expressed pathogen effectors and early-modulated host functions is current...

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Autores principales:	Benjamin Petre, Emmanuelle Morin, Emilie Tisserant, Stéphane Hacquard, Corinne Da Silva, Julie Poulain, Christine Delaruelle, Francis Martin, Nicolas Rouhier, Annegret Kohler, Sébastien Duplessis
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/34e355dd5f52492ba2efca95fb437a5f
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spelling	oai:doaj.org-article:34e355dd5f52492ba2efca95fb437a5f2021-11-18T07:06:59ZRNA-Seq of early-infected poplar leaves by the rust pathogen Melampsora larici-populina uncovers PtSultr3;5, a fungal-induced host sulfate transporter.1932-620310.1371/journal.pone.0044408https://doaj.org/article/34e355dd5f52492ba2efca95fb437a5f2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22952974/?tool=EBIhttps://doaj.org/toc/1932-6203Biotroph pathogens establish intimate interactions with their hosts that are conditioned by the successful secretion of effectors in infected tissues and subsequent manipulation of host physiology. The identification of early-expressed pathogen effectors and early-modulated host functions is currently a major goal to understand the molecular basis of biotrophy. Here, we report the 454-pyrosequencing transcriptome analysis of early stages of poplar leaf colonization by the rust fungus Melampsora larici-populina. Among the 841,301 reads considered for analysis, 616,879 and 649 were successfully mapped to Populus trichocarpa and M. larici-populina genome sequences, respectively. From a methodological aspect, these results indicate that this single approach is not appropriate to saturate poplar transcriptome and to follow transcript accumulation of the pathogen. We identified 19 pathogen transcripts encoding early-expressed small-secreted proteins representing candidate effectors of interest for forthcoming studies. Poplar RNA-Seq data were validated by oligoarrays and quantitatively analysed, which revealed a highly stable transcriptome with a single transcript encoding a sulfate transporter (herein named PtSultr3;5, POPTR_0006s16150) showing a dramatic increase upon colonization by either virulent or avirulent M. larici-populina strains. Perspectives connecting host sulfate transport and biotrophic lifestyle are discussed.Benjamin PetreEmmanuelle MorinEmilie TisserantStéphane HacquardCorinne Da SilvaJulie PoulainChristine DelaruelleFrancis MartinNicolas RouhierAnnegret KohlerSébastien DuplessisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e44408 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Benjamin Petre Emmanuelle Morin Emilie Tisserant Stéphane Hacquard Corinne Da Silva Julie Poulain Christine Delaruelle Francis Martin Nicolas Rouhier Annegret Kohler Sébastien Duplessis RNA-Seq of early-infected poplar leaves by the rust pathogen Melampsora larici-populina uncovers PtSultr3;5, a fungal-induced host sulfate transporter.
description	Biotroph pathogens establish intimate interactions with their hosts that are conditioned by the successful secretion of effectors in infected tissues and subsequent manipulation of host physiology. The identification of early-expressed pathogen effectors and early-modulated host functions is currently a major goal to understand the molecular basis of biotrophy. Here, we report the 454-pyrosequencing transcriptome analysis of early stages of poplar leaf colonization by the rust fungus Melampsora larici-populina. Among the 841,301 reads considered for analysis, 616,879 and 649 were successfully mapped to Populus trichocarpa and M. larici-populina genome sequences, respectively. From a methodological aspect, these results indicate that this single approach is not appropriate to saturate poplar transcriptome and to follow transcript accumulation of the pathogen. We identified 19 pathogen transcripts encoding early-expressed small-secreted proteins representing candidate effectors of interest for forthcoming studies. Poplar RNA-Seq data were validated by oligoarrays and quantitatively analysed, which revealed a highly stable transcriptome with a single transcript encoding a sulfate transporter (herein named PtSultr3;5, POPTR_0006s16150) showing a dramatic increase upon colonization by either virulent or avirulent M. larici-populina strains. Perspectives connecting host sulfate transport and biotrophic lifestyle are discussed.
format	article
author	Benjamin Petre Emmanuelle Morin Emilie Tisserant Stéphane Hacquard Corinne Da Silva Julie Poulain Christine Delaruelle Francis Martin Nicolas Rouhier Annegret Kohler Sébastien Duplessis
author_facet	Benjamin Petre Emmanuelle Morin Emilie Tisserant Stéphane Hacquard Corinne Da Silva Julie Poulain Christine Delaruelle Francis Martin Nicolas Rouhier Annegret Kohler Sébastien Duplessis
author_sort	Benjamin Petre
title	RNA-Seq of early-infected poplar leaves by the rust pathogen Melampsora larici-populina uncovers PtSultr3;5, a fungal-induced host sulfate transporter.
title_short	RNA-Seq of early-infected poplar leaves by the rust pathogen Melampsora larici-populina uncovers PtSultr3;5, a fungal-induced host sulfate transporter.
title_full	RNA-Seq of early-infected poplar leaves by the rust pathogen Melampsora larici-populina uncovers PtSultr3;5, a fungal-induced host sulfate transporter.
title_fullStr	RNA-Seq of early-infected poplar leaves by the rust pathogen Melampsora larici-populina uncovers PtSultr3;5, a fungal-induced host sulfate transporter.
title_full_unstemmed	RNA-Seq of early-infected poplar leaves by the rust pathogen Melampsora larici-populina uncovers PtSultr3;5, a fungal-induced host sulfate transporter.
title_sort	rna-seq of early-infected poplar leaves by the rust pathogen melampsora larici-populina uncovers ptsultr3;5, a fungal-induced host sulfate transporter.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/34e355dd5f52492ba2efca95fb437a5f
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RNA-Seq of early-infected poplar leaves by the rust pathogen Melampsora larici-populina uncovers PtSultr3;5, a fungal-induced host sulfate transporter.

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