Effect of plant growth regulators on in vitro induction and maintenance of callus from leaf and root explants of Atropa acuminata Royal ex Lindl

Atropa acuminata, an important medicinal plant belonging to family Solanaceae is under tremendous threat of extinction in its natural habitat due to the overexploitation by pharmaceutical industries. Present study is an attempt of establishing callus cultures of this important medicinal plant as cal...

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Autores principales:	Shabeer Ahmad Dar, Irshad Ahmad Nawchoo, Sumira Tyub, Azra Nahaid Kamili
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Atropa acuminata In vitro Callus induction Plant growth regulators Explant Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/34e62271d16948399df8ac6230d70a21
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spelling	oai:doaj.org-article:34e62271d16948399df8ac6230d70a212021-11-20T05:06:31ZEffect of plant growth regulators on in vitro induction and maintenance of callus from leaf and root explants of Atropa acuminata Royal ex Lindl2215-017X10.1016/j.btre.2021.e00688https://doaj.org/article/34e62271d16948399df8ac6230d70a212021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2215017X21001041https://doaj.org/toc/2215-017XAtropa acuminata, an important medicinal plant belonging to family Solanaceae is under tremendous threat of extinction in its natural habitat due to the overexploitation by pharmaceutical industries. Present study is an attempt of establishing callus cultures of this important medicinal plant as callus has considerable potential as an alternative for production of secondary metabolites for industrial use, hence reducing pressure on natural populations.Callus cultures were established from leaf and root explants of Atropa acuminate. Murashige and Skoog (MS) media containing different concentration and combinations of 6-Benzyl Amino Purine (BAP), Naphthalene Acetic Acid (NAA), Kinetin (Kn) and 2,4- Dichloropheoxyacetic acid (2,4- D) were used for callus induction. Different phytohormonal combinations resulted in different types and degrees of callus. The combination of BAP and NAA on MS media supplemented with 0.5 mg/l BAP in combination with 1.0 mg/l NAA, was found to be the most efficient for in vitro callus development from root explants and from leaf explants most effective combination and concentration was 1 mg/l of both BAP and NAA. The maximum mean fresh weight of callus formed using root explants was 33.13 mg per explant and maximum fresh weight obtained from leaf explants was 22.14 mg per explants.Shabeer Ahmad DarIrshad Ahmad NawchooSumira TyubAzra Nahaid KamiliElsevierarticleAtropa acuminataIn vitroCallus inductionPlant growth regulatorsExplantBiotechnologyTP248.13-248.65ENBiotechnology Reports, Vol 32, Iss , Pp e00688- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Atropa acuminata In vitro Callus induction Plant growth regulators Explant Biotechnology TP248.13-248.65
spellingShingle	Atropa acuminata In vitro Callus induction Plant growth regulators Explant Biotechnology TP248.13-248.65 Shabeer Ahmad Dar Irshad Ahmad Nawchoo Sumira Tyub Azra Nahaid Kamili Effect of plant growth regulators on in vitro induction and maintenance of callus from leaf and root explants of Atropa acuminata Royal ex Lindl
description	Atropa acuminata, an important medicinal plant belonging to family Solanaceae is under tremendous threat of extinction in its natural habitat due to the overexploitation by pharmaceutical industries. Present study is an attempt of establishing callus cultures of this important medicinal plant as callus has considerable potential as an alternative for production of secondary metabolites for industrial use, hence reducing pressure on natural populations.Callus cultures were established from leaf and root explants of Atropa acuminate. Murashige and Skoog (MS) media containing different concentration and combinations of 6-Benzyl Amino Purine (BAP), Naphthalene Acetic Acid (NAA), Kinetin (Kn) and 2,4- Dichloropheoxyacetic acid (2,4- D) were used for callus induction. Different phytohormonal combinations resulted in different types and degrees of callus. The combination of BAP and NAA on MS media supplemented with 0.5 mg/l BAP in combination with 1.0 mg/l NAA, was found to be the most efficient for in vitro callus development from root explants and from leaf explants most effective combination and concentration was 1 mg/l of both BAP and NAA. The maximum mean fresh weight of callus formed using root explants was 33.13 mg per explant and maximum fresh weight obtained from leaf explants was 22.14 mg per explants.
format	article
author	Shabeer Ahmad Dar Irshad Ahmad Nawchoo Sumira Tyub Azra Nahaid Kamili
author_facet	Shabeer Ahmad Dar Irshad Ahmad Nawchoo Sumira Tyub Azra Nahaid Kamili
author_sort	Shabeer Ahmad Dar
title	Effect of plant growth regulators on in vitro induction and maintenance of callus from leaf and root explants of Atropa acuminata Royal ex Lindl
title_short	Effect of plant growth regulators on in vitro induction and maintenance of callus from leaf and root explants of Atropa acuminata Royal ex Lindl
title_full	Effect of plant growth regulators on in vitro induction and maintenance of callus from leaf and root explants of Atropa acuminata Royal ex Lindl
title_fullStr	Effect of plant growth regulators on in vitro induction and maintenance of callus from leaf and root explants of Atropa acuminata Royal ex Lindl
title_full_unstemmed	Effect of plant growth regulators on in vitro induction and maintenance of callus from leaf and root explants of Atropa acuminata Royal ex Lindl
title_sort	effect of plant growth regulators on in vitro induction and maintenance of callus from leaf and root explants of atropa acuminata royal ex lindl
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/34e62271d16948399df8ac6230d70a21
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Effect of plant growth regulators on in vitro induction and maintenance of callus from leaf and root explants of Atropa acuminata Royal ex Lindl

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