Immune spleen cells attenuate the inflammatory profile of the mesenteric perivascular adipose tissue in obese mice

Abstract The perivascular adipose tissue (PVAT) differs from other fat depots and exerts a paracrine action on the vasculature. The spleen has an important role in the immune response, and it was observed to have either a protective role or a contribution to obesity-related diseases. However, the re...

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Autores principales:	Renée de Nazaré Oliveira da Silva, Rosangela Aparecida Santos-Eichler, Carolina Dias, Stephen Fernandes Rodrigues, Dominik S. Skiba, Richardt Gama Landgraf, Maria Helena Catelli de Carvalho, Tomasz Guzik, Ricardo Ambrósio Fock, Eliana Hiromi Akamine
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/34ecf8a7b588465bb8e04a29337faba6
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spelling	oai:doaj.org-article:34ecf8a7b588465bb8e04a29337faba62021-12-02T15:49:53ZImmune spleen cells attenuate the inflammatory profile of the mesenteric perivascular adipose tissue in obese mice10.1038/s41598-021-90600-02045-2322https://doaj.org/article/34ecf8a7b588465bb8e04a29337faba62021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90600-0https://doaj.org/toc/2045-2322Abstract The perivascular adipose tissue (PVAT) differs from other fat depots and exerts a paracrine action on the vasculature. The spleen has an important role in the immune response, and it was observed to have either a protective role or a contribution to obesity-related diseases. However, the relation between spleen and PVAT is elusive in obesity. We investigated the role of spleen in the inflammatory profile of the mesenteric PVAT (mPVAT) from mice fed a high-fat diet (HFD) for 16 weeks. Male C57Bl/6 mice were sham-operated or splenectomized (SPX) and fed a HFD for 16 weeks. mPVAT morphology was evaluated by hematoxylin and eosin staining, infiltrated immune cells were evaluated by flow cytometry, inflammatory cytokines were evaluated by ELISA and the splenic cell chemotaxis mediated by mPVAT was evaluated using a transwell assay. In SPX mice, HFD induced adipocyte hypertrophy and increased immune cell infiltration and proinflammatory cytokine levels in mPVAT. However, none of these effects were observed in mPVAT from sham-operated mice. Spleen from HFD fed mice presented reduced total leukocytes and increased inflammatory markers when compared to the spleen from control mice. Chemotaxis of spleen cells mediated by mPVAT of HFD fed mice was reduced in relation to standard diet fed mice. The spleen protects mPVAT against the effects of 16-week HFD. This information was missing, and it is important because PVAT is different from other fat depots and data cannot be extrapolated from any type of adipose tissue to PVAT.Renée de Nazaré Oliveira da SilvaRosangela Aparecida Santos-EichlerCarolina DiasStephen Fernandes RodriguesDominik S. SkibaRichardt Gama LandgrafMaria Helena Catelli de CarvalhoTomasz GuzikRicardo Ambrósio FockEliana Hiromi AkamineNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Renée de Nazaré Oliveira da Silva Rosangela Aparecida Santos-Eichler Carolina Dias Stephen Fernandes Rodrigues Dominik S. Skiba Richardt Gama Landgraf Maria Helena Catelli de Carvalho Tomasz Guzik Ricardo Ambrósio Fock Eliana Hiromi Akamine Immune spleen cells attenuate the inflammatory profile of the mesenteric perivascular adipose tissue in obese mice
description	Abstract The perivascular adipose tissue (PVAT) differs from other fat depots and exerts a paracrine action on the vasculature. The spleen has an important role in the immune response, and it was observed to have either a protective role or a contribution to obesity-related diseases. However, the relation between spleen and PVAT is elusive in obesity. We investigated the role of spleen in the inflammatory profile of the mesenteric PVAT (mPVAT) from mice fed a high-fat diet (HFD) for 16 weeks. Male C57Bl/6 mice were sham-operated or splenectomized (SPX) and fed a HFD for 16 weeks. mPVAT morphology was evaluated by hematoxylin and eosin staining, infiltrated immune cells were evaluated by flow cytometry, inflammatory cytokines were evaluated by ELISA and the splenic cell chemotaxis mediated by mPVAT was evaluated using a transwell assay. In SPX mice, HFD induced adipocyte hypertrophy and increased immune cell infiltration and proinflammatory cytokine levels in mPVAT. However, none of these effects were observed in mPVAT from sham-operated mice. Spleen from HFD fed mice presented reduced total leukocytes and increased inflammatory markers when compared to the spleen from control mice. Chemotaxis of spleen cells mediated by mPVAT of HFD fed mice was reduced in relation to standard diet fed mice. The spleen protects mPVAT against the effects of 16-week HFD. This information was missing, and it is important because PVAT is different from other fat depots and data cannot be extrapolated from any type of adipose tissue to PVAT.
format	article
author	Renée de Nazaré Oliveira da Silva Rosangela Aparecida Santos-Eichler Carolina Dias Stephen Fernandes Rodrigues Dominik S. Skiba Richardt Gama Landgraf Maria Helena Catelli de Carvalho Tomasz Guzik Ricardo Ambrósio Fock Eliana Hiromi Akamine
author_facet	Renée de Nazaré Oliveira da Silva Rosangela Aparecida Santos-Eichler Carolina Dias Stephen Fernandes Rodrigues Dominik S. Skiba Richardt Gama Landgraf Maria Helena Catelli de Carvalho Tomasz Guzik Ricardo Ambrósio Fock Eliana Hiromi Akamine
author_sort	Renée de Nazaré Oliveira da Silva
title	Immune spleen cells attenuate the inflammatory profile of the mesenteric perivascular adipose tissue in obese mice
title_short	Immune spleen cells attenuate the inflammatory profile of the mesenteric perivascular adipose tissue in obese mice
title_full	Immune spleen cells attenuate the inflammatory profile of the mesenteric perivascular adipose tissue in obese mice
title_fullStr	Immune spleen cells attenuate the inflammatory profile of the mesenteric perivascular adipose tissue in obese mice
title_full_unstemmed	Immune spleen cells attenuate the inflammatory profile of the mesenteric perivascular adipose tissue in obese mice
title_sort	immune spleen cells attenuate the inflammatory profile of the mesenteric perivascular adipose tissue in obese mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/34ecf8a7b588465bb8e04a29337faba6
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Immune spleen cells attenuate the inflammatory profile of the mesenteric perivascular adipose tissue in obese mice

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