Effect of 17β-estradiol on a human vaginal Lactobacillus crispatus strain

Abstract Lactobacilli and estrogens play essential roles in vaginal homeostasis. We investigated the potential direct effect of 17β-estradiol on a vaginal strain of Lactobacillus crispatus, the major bacterial species of the vaginal microbiota. 17β-estradiol (10–6 to 10–10 M) had no effect on L. cri...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Maximilien Clabaut, Amandine Suet, Pierre-Jean Racine, Ali Tahrioui, Julien Verdon, Magalie Barreau, Olivier Maillot, Agathe Le Tirant, Madina Karsybayeva, Coralie Kremser, Gérard Redziniak, Cécile Duclairoir-Poc, Chantal Pichon, Sylvie Chevalier, Marc G. J. Feuilloley
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/34f1b9c2a7964b5ba745f9c9e7afed38
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:34f1b9c2a7964b5ba745f9c9e7afed38
record_format	dspace
spelling	oai:doaj.org-article:34f1b9c2a7964b5ba745f9c9e7afed382021-12-02T14:23:04ZEffect of 17β-estradiol on a human vaginal Lactobacillus crispatus strain10.1038/s41598-021-86628-x2045-2322https://doaj.org/article/34f1b9c2a7964b5ba745f9c9e7afed382021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86628-xhttps://doaj.org/toc/2045-2322Abstract Lactobacilli and estrogens play essential roles in vaginal homeostasis. We investigated the potential direct effect of 17β-estradiol on a vaginal strain of Lactobacillus crispatus, the major bacterial species of the vaginal microbiota. 17β-estradiol (10–6 to 10–10 M) had no effect on L. crispatus growth, but markedly affected the membrane dynamics of this bacterium. This effect appeared consistent with a signal transduction process. The surface polarity and aggregation potential of the bacterium were unaffected by exposure to 17β-estradiol, but its mean size was significantly reduced. 17β-estradiol also promoted biosurfactant production by L. crispatus and adhesion to vaginal VK2/E6E7 cells, but had little effect on bacterial biofilm formation activity. Bioinformatic analysis of L. crispatus identified a membrane lipid raft–associated stomatin/prohibitin/flotillin/HflK domain containing protein as a potential 17β-estradiol binding site. Overall, our results reveal direct effects of 17β-estradiol on L. crispatus. These effects are of potential importance in the physiology of the vaginal environment, through the promotion of lactobacillus adhesion to the mucosa and protection against pathogens.Maximilien ClabautAmandine SuetPierre-Jean RacineAli TahriouiJulien VerdonMagalie BarreauOlivier MaillotAgathe Le TirantMadina KarsybayevaCoralie KremserGérard RedziniakCécile Duclairoir-PocChantal PichonSylvie ChevalierMarc G. J. FeuilloleyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maximilien Clabaut Amandine Suet Pierre-Jean Racine Ali Tahrioui Julien Verdon Magalie Barreau Olivier Maillot Agathe Le Tirant Madina Karsybayeva Coralie Kremser Gérard Redziniak Cécile Duclairoir-Poc Chantal Pichon Sylvie Chevalier Marc G. J. Feuilloley Effect of 17β-estradiol on a human vaginal Lactobacillus crispatus strain
description	Abstract Lactobacilli and estrogens play essential roles in vaginal homeostasis. We investigated the potential direct effect of 17β-estradiol on a vaginal strain of Lactobacillus crispatus, the major bacterial species of the vaginal microbiota. 17β-estradiol (10–6 to 10–10 M) had no effect on L. crispatus growth, but markedly affected the membrane dynamics of this bacterium. This effect appeared consistent with a signal transduction process. The surface polarity and aggregation potential of the bacterium were unaffected by exposure to 17β-estradiol, but its mean size was significantly reduced. 17β-estradiol also promoted biosurfactant production by L. crispatus and adhesion to vaginal VK2/E6E7 cells, but had little effect on bacterial biofilm formation activity. Bioinformatic analysis of L. crispatus identified a membrane lipid raft–associated stomatin/prohibitin/flotillin/HflK domain containing protein as a potential 17β-estradiol binding site. Overall, our results reveal direct effects of 17β-estradiol on L. crispatus. These effects are of potential importance in the physiology of the vaginal environment, through the promotion of lactobacillus adhesion to the mucosa and protection against pathogens.
format	article
author	Maximilien Clabaut Amandine Suet Pierre-Jean Racine Ali Tahrioui Julien Verdon Magalie Barreau Olivier Maillot Agathe Le Tirant Madina Karsybayeva Coralie Kremser Gérard Redziniak Cécile Duclairoir-Poc Chantal Pichon Sylvie Chevalier Marc G. J. Feuilloley
author_facet	Maximilien Clabaut Amandine Suet Pierre-Jean Racine Ali Tahrioui Julien Verdon Magalie Barreau Olivier Maillot Agathe Le Tirant Madina Karsybayeva Coralie Kremser Gérard Redziniak Cécile Duclairoir-Poc Chantal Pichon Sylvie Chevalier Marc G. J. Feuilloley
author_sort	Maximilien Clabaut
title	Effect of 17β-estradiol on a human vaginal Lactobacillus crispatus strain
title_short	Effect of 17β-estradiol on a human vaginal Lactobacillus crispatus strain
title_full	Effect of 17β-estradiol on a human vaginal Lactobacillus crispatus strain
title_fullStr	Effect of 17β-estradiol on a human vaginal Lactobacillus crispatus strain
title_full_unstemmed	Effect of 17β-estradiol on a human vaginal Lactobacillus crispatus strain
title_sort	effect of 17β-estradiol on a human vaginal lactobacillus crispatus strain
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/34f1b9c2a7964b5ba745f9c9e7afed38
work_keys_str_mv	AT maximilienclabaut effectof17bestradiolonahumanvaginallactobacilluscrispatusstrain AT amandinesuet effectof17bestradiolonahumanvaginallactobacilluscrispatusstrain AT pierrejeanracine effectof17bestradiolonahumanvaginallactobacilluscrispatusstrain AT alitahrioui effectof17bestradiolonahumanvaginallactobacilluscrispatusstrain AT julienverdon effectof17bestradiolonahumanvaginallactobacilluscrispatusstrain AT magaliebarreau effectof17bestradiolonahumanvaginallactobacilluscrispatusstrain AT oliviermaillot effectof17bestradiolonahumanvaginallactobacilluscrispatusstrain AT agatheletirant effectof17bestradiolonahumanvaginallactobacilluscrispatusstrain AT madinakarsybayeva effectof17bestradiolonahumanvaginallactobacilluscrispatusstrain AT coraliekremser effectof17bestradiolonahumanvaginallactobacilluscrispatusstrain AT gerardredziniak effectof17bestradiolonahumanvaginallactobacilluscrispatusstrain AT cecileduclairoirpoc effectof17bestradiolonahumanvaginallactobacilluscrispatusstrain AT chantalpichon effectof17bestradiolonahumanvaginallactobacilluscrispatusstrain AT sylviechevalier effectof17bestradiolonahumanvaginallactobacilluscrispatusstrain AT marcgjfeuilloley effectof17bestradiolonahumanvaginallactobacilluscrispatusstrain
_version_	1718391477093007360

Effect of 17β-estradiol on a human vaginal Lactobacillus crispatus strain

Ejemplares similares