Matrix metalloproteinase-9 and p53 involved in chronic fluorosis induced blood-brain barrier damage and neurocyte changes

Introduction A large number of basic and clinical studies have confirmed that fluoride produces toxic effects on multiple organ systems in the body including the nervous system. Material and methods One hundred twenty Wistar rats were randomly divided into 4 groups with 30 in each group: a high flu...

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Autores principales:	Shen Qing-Feng, Xia Ying-Peng, Xu Tian-Tong
Formato:	article
Lenguaje:	EN
Publicado:	Termedia Publishing House 2019
Materias:	p53 rats mmp-9 fluorosis nerve cells blood brain barrier blood-brain barrier matrix metalloproteinase-9 Medicine R
Acceso en línea:	https://doaj.org/article/34f27503d53947549daa8aecfae0528d
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spelling	oai:doaj.org-article:34f27503d53947549daa8aecfae0528d2021-12-02T18:33:05ZMatrix metalloproteinase-9 and p53 involved in chronic fluorosis induced blood-brain barrier damage and neurocyte changes1734-19221896-915110.5114/aoms.2019.83294https://doaj.org/article/34f27503d53947549daa8aecfae0528d2019-03-01T00:00:00Zhttps://www.archivesofmedicalscience.com/Matrix-metalloproteinase-9-and-p53-involved-in-chronic-fluorosis-induced-blood-brain,69040,0,2.htmlhttps://doaj.org/toc/1734-1922https://doaj.org/toc/1896-9151Introduction A large number of basic and clinical studies have confirmed that fluoride produces toxic effects on multiple organ systems in the body including the nervous system. Material and methods One hundred twenty Wistar rats were randomly divided into 4 groups with 30 in each group: a high fluoride group (drinking 200 mg/l fluoridated water, 24 weeks); a high fluoride control group (drinking distilled water, 24 weeks); a fluoride removal group (drinking fluoridated water, 12 W; then distilled water, 12 W) and a defluorination control group (drinking distilled water, 24 weeks). Results The high fluoride and fluoride removal groups had spinal cord astrocyte edema. The apoptosis rate of spinal nerve cells in the high fluoride group and fluoride removal group were significantly higher (p < 0.01) than in the fluoride control and defluorination control group. The Evans blue (EB) content, matrix metalloproteinase-9 (MMP-9) and p53 expression in the high fluoride group and fluoride removal group were higher (p < 0.01) than in the fluoride control and defluorination control group. Conclusions The apoptosis of spinal cord nerve cells is obviously higher in rats with chronic fluoride exposure. Chronic fluoride exposure leads to high expression of MMP-9, and results in increased damage of the blood-spinal cord barrier. Increased p53 may be one of the factors causing damage. Short-term removal of fluoride has no obvious recovery in apoptosis of spinal cord nerve cells; highly expressed MMP-9 and p53 may be one of the reasons for unrecovered function.Shen Qing-FengXia Ying-PengXu Tian-TongTermedia Publishing Housearticlep53ratsmmp-9fluorosisnerve cellsblood brain barrierfluorosisblood-brain barriernerve cellsmatrix metalloproteinase-9MedicineRENArchives of Medical Science, Vol 15, Iss 2, Pp 457-466 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	p53 rats mmp-9 fluorosis nerve cells blood brain barrier fluorosis blood-brain barrier nerve cells matrix metalloproteinase-9 Medicine R
spellingShingle	p53 rats mmp-9 fluorosis nerve cells blood brain barrier fluorosis blood-brain barrier nerve cells matrix metalloproteinase-9 Medicine R Shen Qing-Feng Xia Ying-Peng Xu Tian-Tong Matrix metalloproteinase-9 and p53 involved in chronic fluorosis induced blood-brain barrier damage and neurocyte changes
description	Introduction A large number of basic and clinical studies have confirmed that fluoride produces toxic effects on multiple organ systems in the body including the nervous system. Material and methods One hundred twenty Wistar rats were randomly divided into 4 groups with 30 in each group: a high fluoride group (drinking 200 mg/l fluoridated water, 24 weeks); a high fluoride control group (drinking distilled water, 24 weeks); a fluoride removal group (drinking fluoridated water, 12 W; then distilled water, 12 W) and a defluorination control group (drinking distilled water, 24 weeks). Results The high fluoride and fluoride removal groups had spinal cord astrocyte edema. The apoptosis rate of spinal nerve cells in the high fluoride group and fluoride removal group were significantly higher (p < 0.01) than in the fluoride control and defluorination control group. The Evans blue (EB) content, matrix metalloproteinase-9 (MMP-9) and p53 expression in the high fluoride group and fluoride removal group were higher (p < 0.01) than in the fluoride control and defluorination control group. Conclusions The apoptosis of spinal cord nerve cells is obviously higher in rats with chronic fluoride exposure. Chronic fluoride exposure leads to high expression of MMP-9, and results in increased damage of the blood-spinal cord barrier. Increased p53 may be one of the factors causing damage. Short-term removal of fluoride has no obvious recovery in apoptosis of spinal cord nerve cells; highly expressed MMP-9 and p53 may be one of the reasons for unrecovered function.
format	article
author	Shen Qing-Feng Xia Ying-Peng Xu Tian-Tong
author_facet	Shen Qing-Feng Xia Ying-Peng Xu Tian-Tong
author_sort	Shen Qing-Feng
title	Matrix metalloproteinase-9 and p53 involved in chronic fluorosis induced blood-brain barrier damage and neurocyte changes
title_short	Matrix metalloproteinase-9 and p53 involved in chronic fluorosis induced blood-brain barrier damage and neurocyte changes
title_full	Matrix metalloproteinase-9 and p53 involved in chronic fluorosis induced blood-brain barrier damage and neurocyte changes
title_fullStr	Matrix metalloproteinase-9 and p53 involved in chronic fluorosis induced blood-brain barrier damage and neurocyte changes
title_full_unstemmed	Matrix metalloproteinase-9 and p53 involved in chronic fluorosis induced blood-brain barrier damage and neurocyte changes
title_sort	matrix metalloproteinase-9 and p53 involved in chronic fluorosis induced blood-brain barrier damage and neurocyte changes
publisher	Termedia Publishing House
publishDate	2019
url	https://doaj.org/article/34f27503d53947549daa8aecfae0528d
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Matrix metalloproteinase-9 and p53 involved in chronic fluorosis induced blood-brain barrier damage and neurocyte changes

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