Fe3O4-based PLGA nanoparticles as MR contrast agents for the detection of thrombosis

Jia Liu,1 Jie Xu,1 Jun Zhou,1 Yu Zhang,1 Dajing Guo,1 Zhigang Wang2 1Department of Radiology, 2Department of Ultrasound, Institute of Ultrasound Imaging, The Second Affiliated Hospital of Chongqing Medical University, Yuzhong, Chongqing, People’s Republic of China Abstract: Thrombotic di...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Liu J, Xu J, Zhou J, Zhang Y, Guo D, Wang Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Iron oxide Poly (lactic-co-glycolic acid) Thrombosis Magnetic resonance imaging Cyclic Arg-Gly-Asp peptide Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/34f40cac7e1647019b2f6cda1595dabd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:34f40cac7e1647019b2f6cda1595dabd
record_format	dspace
spelling	oai:doaj.org-article:34f40cac7e1647019b2f6cda1595dabd2021-12-02T05:36:20ZFe3O4-based PLGA nanoparticles as MR contrast agents for the detection of thrombosis1178-2013https://doaj.org/article/34f40cac7e1647019b2f6cda1595dabd2017-02-01T00:00:00Zhttps://www.dovepress.com/fe3o4-based-plga-nanoparticles-as-mr-contrast-agents-for-the-detection-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jia Liu,1 Jie Xu,1 Jun Zhou,1 Yu Zhang,1 Dajing Guo,1 Zhigang Wang2 1Department of Radiology, 2Department of Ultrasound, Institute of Ultrasound Imaging, The Second Affiliated Hospital of Chongqing Medical University, Yuzhong, Chongqing, People’s Republic of China Abstract: Thrombotic disease is a great threat to human health, and early detection is particularly important. Magnetic resonance (MR) molecular imaging provides noninvasive imaging with the potential for early disease diagnosis. In this study, we developed Fe3O4-based poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) surface-modified with a cyclic Arg-Gly-Asp (cRGD) peptide as an MR contrast agent for the detection of thrombosis. The physical and chemical characteristics, biological toxicity, ability to target thrombi, and biodistribution of the NPs were studied. The Fe3O4-PLGA-cRGD NPs were constructed successfully, and hematologic and pathologic assays indicated no in vivo toxicity of the NPs. In a rat model of FeCl3-induced abdominal aorta thrombosis, the NPs readily and selectively accumulated on the surface of the thrombosis and under vascular endothelial cells ex vivo and in vivo. In the in vivo experiment, the biodistribution of the NPs suggested that the NPs might be internalized by the macrophages of the reticuloendothelial system in the liver and the spleen. The T2 signal decreased at the mural thrombus 10 min after injection and then gradually increased until 50 min. These results suggest that the NPs are suitable for in vivo molecular imaging of thrombosis under high shear stress conditions and represent a very promising MR contrast agent for sensitive and specific detection of thrombosis. Keywords: iron oxide, poly(lactic-co-glycolic acid), thrombosis, magnetic resonance imaging, cyclic Arg-Gly-Asp peptideLiu JXu JZhou JZhang YGuo DWang ZDove Medical PressarticleIron oxidePoly (lactic-co-glycolic acid)ThrombosisMagnetic resonance imagingCyclic Arg-Gly-Asp peptideMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 1113-1126 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Iron oxide Poly (lactic-co-glycolic acid) Thrombosis Magnetic resonance imaging Cyclic Arg-Gly-Asp peptide Medicine (General) R5-920
spellingShingle	Iron oxide Poly (lactic-co-glycolic acid) Thrombosis Magnetic resonance imaging Cyclic Arg-Gly-Asp peptide Medicine (General) R5-920 Liu J Xu J Zhou J Zhang Y Guo D Wang Z Fe3O4-based PLGA nanoparticles as MR contrast agents for the detection of thrombosis
description	Jia Liu,1 Jie Xu,1 Jun Zhou,1 Yu Zhang,1 Dajing Guo,1 Zhigang Wang2 1Department of Radiology, 2Department of Ultrasound, Institute of Ultrasound Imaging, The Second Affiliated Hospital of Chongqing Medical University, Yuzhong, Chongqing, People’s Republic of China Abstract: Thrombotic disease is a great threat to human health, and early detection is particularly important. Magnetic resonance (MR) molecular imaging provides noninvasive imaging with the potential for early disease diagnosis. In this study, we developed Fe3O4-based poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) surface-modified with a cyclic Arg-Gly-Asp (cRGD) peptide as an MR contrast agent for the detection of thrombosis. The physical and chemical characteristics, biological toxicity, ability to target thrombi, and biodistribution of the NPs were studied. The Fe3O4-PLGA-cRGD NPs were constructed successfully, and hematologic and pathologic assays indicated no in vivo toxicity of the NPs. In a rat model of FeCl3-induced abdominal aorta thrombosis, the NPs readily and selectively accumulated on the surface of the thrombosis and under vascular endothelial cells ex vivo and in vivo. In the in vivo experiment, the biodistribution of the NPs suggested that the NPs might be internalized by the macrophages of the reticuloendothelial system in the liver and the spleen. The T2 signal decreased at the mural thrombus 10 min after injection and then gradually increased until 50 min. These results suggest that the NPs are suitable for in vivo molecular imaging of thrombosis under high shear stress conditions and represent a very promising MR contrast agent for sensitive and specific detection of thrombosis. Keywords: iron oxide, poly(lactic-co-glycolic acid), thrombosis, magnetic resonance imaging, cyclic Arg-Gly-Asp peptide
format	article
author	Liu J Xu J Zhou J Zhang Y Guo D Wang Z
author_facet	Liu J Xu J Zhou J Zhang Y Guo D Wang Z
author_sort	Liu J
title	Fe3O4-based PLGA nanoparticles as MR contrast agents for the detection of thrombosis
title_short	Fe3O4-based PLGA nanoparticles as MR contrast agents for the detection of thrombosis
title_full	Fe3O4-based PLGA nanoparticles as MR contrast agents for the detection of thrombosis
title_fullStr	Fe3O4-based PLGA nanoparticles as MR contrast agents for the detection of thrombosis
title_full_unstemmed	Fe3O4-based PLGA nanoparticles as MR contrast agents for the detection of thrombosis
title_sort	fe3o4-based plga nanoparticles as mr contrast agents for the detection of thrombosis
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/34f40cac7e1647019b2f6cda1595dabd
work_keys_str_mv	AT liuj fe3o4basedplgananoparticlesasmrcontrastagentsforthedetectionofthrombosis AT xuj fe3o4basedplgananoparticlesasmrcontrastagentsforthedetectionofthrombosis AT zhouj fe3o4basedplgananoparticlesasmrcontrastagentsforthedetectionofthrombosis AT zhangy fe3o4basedplgananoparticlesasmrcontrastagentsforthedetectionofthrombosis AT guod fe3o4basedplgananoparticlesasmrcontrastagentsforthedetectionofthrombosis AT wangz fe3o4basedplgananoparticlesasmrcontrastagentsforthedetectionofthrombosis
_version_	1718400287764381696

Fe3O4-based PLGA nanoparticles as MR contrast agents for the detection of thrombosis

Ejemplares similares