Short Communication: Investigation of Diphtheria in Indonesia: dtxR and tox genes analysis of Corynebacterium diphtheriae collected from outbreaks

Mulyastuti Y, Rahayu SI, Sunarno S, Santoso S, Wasito EB. 2017. Short Communication: Investigation of Diphtheria in Indonesia: dtxR and tox genes analysis of Corynebacterium diphtheriae collected from outbreaks. Biodiversitas 18: 784-787. Diphtheria outbreaks have sporadically occurred in Indonesia...

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Autores principales:	YUANITA MULYASTUTI, SIWIPENI I. RAHAYU, SUNARNO SUNARNO, EDDY B. WASITO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2017
Materias:	corynebacterium diphtheriae dna sequence analysis dtxr gene tox gene indonesia Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/34f5600d8ff54cb4a3eef83a0b1a39be
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spelling	oai:doaj.org-article:34f5600d8ff54cb4a3eef83a0b1a39be2021-11-16T13:52:30ZShort Communication: Investigation of Diphtheria in Indonesia: dtxR and tox genes analysis of Corynebacterium diphtheriae collected from outbreaks1412-033X2085-472210.13057/biodiv/d180247https://doaj.org/article/34f5600d8ff54cb4a3eef83a0b1a39be2017-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/1706https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Mulyastuti Y, Rahayu SI, Sunarno S, Santoso S, Wasito EB. 2017. Short Communication: Investigation of Diphtheria in Indonesia: dtxR and tox genes analysis of Corynebacterium diphtheriae collected from outbreaks. Biodiversitas 18: 784-787. Diphtheria outbreaks have sporadically occurred in Indonesia recently. It is suspected that toxin profile changes play an important role in vaccine efficacy and the occurrence of outbreaks. This study aimed to investigate the genetic changes of dtxR and tox genes in Corynebacterium diphtheria in Indonesia. Four C. diphtheriae toxigenic isolates circulating in current outbreak area were analyzed by comparing DNA sequences of their dtxR and tox genes to those of the PW8 vaccine strain. Among the four isolates, three point mutations were detected in dtxR gene while three other point mutations were detected in the tox gene. All six were silent mutations, suggesting that the diphtheria toxin is highly conserved at the amino acid sequence level, and indirectly indicating that the vaccine remains appropriate. Genetic variation in dtxR and tox genes of C. diphtheriae isolates from the recent outbreaks in Indonesia was detected.YUANITA MULYASTUTISIWIPENI I. RAHAYUSUNARNO SUNARNOEDDY B. WASITOMBI & UNS Soloarticlecorynebacterium diphtheriaedna sequence analysisdtxr genetox geneindonesiaBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 2, Pp 784-787 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	corynebacterium diphtheriae dna sequence analysis dtxr gene tox gene indonesia Biology (General) QH301-705.5
spellingShingle	corynebacterium diphtheriae dna sequence analysis dtxr gene tox gene indonesia Biology (General) QH301-705.5 YUANITA MULYASTUTI SIWIPENI I. RAHAYU SUNARNO SUNARNO EDDY B. WASITO Short Communication: Investigation of Diphtheria in Indonesia: dtxR and tox genes analysis of Corynebacterium diphtheriae collected from outbreaks
description	Mulyastuti Y, Rahayu SI, Sunarno S, Santoso S, Wasito EB. 2017. Short Communication: Investigation of Diphtheria in Indonesia: dtxR and tox genes analysis of Corynebacterium diphtheriae collected from outbreaks. Biodiversitas 18: 784-787. Diphtheria outbreaks have sporadically occurred in Indonesia recently. It is suspected that toxin profile changes play an important role in vaccine efficacy and the occurrence of outbreaks. This study aimed to investigate the genetic changes of dtxR and tox genes in Corynebacterium diphtheria in Indonesia. Four C. diphtheriae toxigenic isolates circulating in current outbreak area were analyzed by comparing DNA sequences of their dtxR and tox genes to those of the PW8 vaccine strain. Among the four isolates, three point mutations were detected in dtxR gene while three other point mutations were detected in the tox gene. All six were silent mutations, suggesting that the diphtheria toxin is highly conserved at the amino acid sequence level, and indirectly indicating that the vaccine remains appropriate. Genetic variation in dtxR and tox genes of C. diphtheriae isolates from the recent outbreaks in Indonesia was detected.
format	article
author	YUANITA MULYASTUTI SIWIPENI I. RAHAYU SUNARNO SUNARNO EDDY B. WASITO
author_facet	YUANITA MULYASTUTI SIWIPENI I. RAHAYU SUNARNO SUNARNO EDDY B. WASITO
author_sort	YUANITA MULYASTUTI
title	Short Communication: Investigation of Diphtheria in Indonesia: dtxR and tox genes analysis of Corynebacterium diphtheriae collected from outbreaks
title_short	Short Communication: Investigation of Diphtheria in Indonesia: dtxR and tox genes analysis of Corynebacterium diphtheriae collected from outbreaks
title_full	Short Communication: Investigation of Diphtheria in Indonesia: dtxR and tox genes analysis of Corynebacterium diphtheriae collected from outbreaks
title_fullStr	Short Communication: Investigation of Diphtheria in Indonesia: dtxR and tox genes analysis of Corynebacterium diphtheriae collected from outbreaks
title_full_unstemmed	Short Communication: Investigation of Diphtheria in Indonesia: dtxR and tox genes analysis of Corynebacterium diphtheriae collected from outbreaks
title_sort	short communication: investigation of diphtheria in indonesia: dtxr and tox genes analysis of corynebacterium diphtheriae collected from outbreaks
publisher	MBI & UNS Solo
publishDate	2017
url	https://doaj.org/article/34f5600d8ff54cb4a3eef83a0b1a39be
work_keys_str_mv	AT yuanitamulyastuti shortcommunicationinvestigationofdiphtheriainindonesiadtxrandtoxgenesanalysisofcorynebacteriumdiphtheriaecollectedfromoutbreaks AT siwipeniirahayu shortcommunicationinvestigationofdiphtheriainindonesiadtxrandtoxgenesanalysisofcorynebacteriumdiphtheriaecollectedfromoutbreaks AT sunarnosunarno shortcommunicationinvestigationofdiphtheriainindonesiadtxrandtoxgenesanalysisofcorynebacteriumdiphtheriaecollectedfromoutbreaks AT eddybwasito shortcommunicationinvestigationofdiphtheriainindonesiadtxrandtoxgenesanalysisofcorynebacteriumdiphtheriaecollectedfromoutbreaks
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Short Communication: Investigation of Diphtheria in Indonesia: dtxR and tox genes analysis of Corynebacterium diphtheriae collected from outbreaks

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