Platinum Nanoparticles As A Therapeutic Agent Against Dextran Sodium Sulfate-Induced Colitis In Mice

Suqin Zhu,1,2 Mingyong Zeng,2 Guangxin Feng,2 Haohao Wu2 1Institute of Nutrition and Health, Qingdao University, Qingdao 266021, People’s Republic of China; 2College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, 266003, People’s Republic...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhu S, Zeng M, Feng G, Wu H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	platinum nanoparticles ulcerative colitis gut microbiota anti-inflammation gut barrier function. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/34f950b00ca44de7a0f2a134d7e77027
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:34f950b00ca44de7a0f2a134d7e77027
record_format	dspace
spelling	oai:doaj.org-article:34f950b00ca44de7a0f2a134d7e770272021-12-02T04:01:22ZPlatinum Nanoparticles As A Therapeutic Agent Against Dextran Sodium Sulfate-Induced Colitis In Mice1178-2013https://doaj.org/article/34f950b00ca44de7a0f2a134d7e770272019-10-01T00:00:00Zhttps://www.dovepress.com/platinum-nanoparticles-as-a-therapeutic-agent-against-dextran-sodium-s-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Suqin Zhu,1,2 Mingyong Zeng,2 Guangxin Feng,2 Haohao Wu2 1Institute of Nutrition and Health, Qingdao University, Qingdao 266021, People’s Republic of China; 2College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, 266003, People’s Republic of ChinaCorrespondence: Haohao WuCollege of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong Province 266003, People’s Republic of ChinaTel +86-532-82032400 Fax +86-532-82032400 Email wuhaohao@ouc.edu.cnPurpose: This study aimed to evaluate the anti-colitis potential of platinum nanoparticles (PtNPs).Materials and methods: 5-, 30- and 70-nm PtNPs were administered to C57BL/6 mice once daily by intragastric gavage for 8 d during and after 5-d dextran sodium sulfate treatment.Results: According to body weight change, stool blood and consistency, and colon length and histopathology, PtNPs size-dependently alleviated DSS-induced murine colitis. PtNPs enhanced gut-barrier function by upregulating the colonic expressions of heat-shock protein 25 and tight junction proteins. Based on colonic myeloperoxidase activity, colonic and peripheral levels of interleukin-6 and tumor necrosis factor-α, and peripheral counts of white blood cells, PtNPs attenuated colonic and systemic inflammation. By suppressing lipopolysaccharide-triggered production of proinflammatory mediators, including nitric oxide, tumor necrosis factor-α and interleukin-6, PtNPs exerted direct anti-inflammatory activities in RAW264.7 macrophages through a mechanism involving intracellular reactive oxygen species scavenging and Toll-like receptor 4/NF-κB signaling suppression. High-throughput 16S rRNA sequencing of fecal samples unveiled that PtNPs induced gut dysbiosis by unfavorably altering α-diversity, Firmicutes/Bacteroidetes ratio, and richness of certain specific bacteria.Conclusion: PtNPs are a promising anti-colitis agent, but may negatively impact gut-microbiota.Keywords: platinum nanoparticles, ulcerative colitis, gut-microbiota, anti-inflammation, gut-barrier functionZhu SZeng MFeng GWu HDove Medical Pressarticleplatinum nanoparticlesulcerative colitisgut microbiotaanti-inflammationgut barrier function.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 8361-8378 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	platinum nanoparticles ulcerative colitis gut microbiota anti-inflammation gut barrier function. Medicine (General) R5-920
spellingShingle	platinum nanoparticles ulcerative colitis gut microbiota anti-inflammation gut barrier function. Medicine (General) R5-920 Zhu S Zeng M Feng G Wu H Platinum Nanoparticles As A Therapeutic Agent Against Dextran Sodium Sulfate-Induced Colitis In Mice
description	Suqin Zhu,1,2 Mingyong Zeng,2 Guangxin Feng,2 Haohao Wu2 1Institute of Nutrition and Health, Qingdao University, Qingdao 266021, People’s Republic of China; 2College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, 266003, People’s Republic of ChinaCorrespondence: Haohao WuCollege of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong Province 266003, People’s Republic of ChinaTel +86-532-82032400 Fax +86-532-82032400 Email wuhaohao@ouc.edu.cnPurpose: This study aimed to evaluate the anti-colitis potential of platinum nanoparticles (PtNPs).Materials and methods: 5-, 30- and 70-nm PtNPs were administered to C57BL/6 mice once daily by intragastric gavage for 8 d during and after 5-d dextran sodium sulfate treatment.Results: According to body weight change, stool blood and consistency, and colon length and histopathology, PtNPs size-dependently alleviated DSS-induced murine colitis. PtNPs enhanced gut-barrier function by upregulating the colonic expressions of heat-shock protein 25 and tight junction proteins. Based on colonic myeloperoxidase activity, colonic and peripheral levels of interleukin-6 and tumor necrosis factor-α, and peripheral counts of white blood cells, PtNPs attenuated colonic and systemic inflammation. By suppressing lipopolysaccharide-triggered production of proinflammatory mediators, including nitric oxide, tumor necrosis factor-α and interleukin-6, PtNPs exerted direct anti-inflammatory activities in RAW264.7 macrophages through a mechanism involving intracellular reactive oxygen species scavenging and Toll-like receptor 4/NF-κB signaling suppression. High-throughput 16S rRNA sequencing of fecal samples unveiled that PtNPs induced gut dysbiosis by unfavorably altering α-diversity, Firmicutes/Bacteroidetes ratio, and richness of certain specific bacteria.Conclusion: PtNPs are a promising anti-colitis agent, but may negatively impact gut-microbiota.Keywords: platinum nanoparticles, ulcerative colitis, gut-microbiota, anti-inflammation, gut-barrier function
format	article
author	Zhu S Zeng M Feng G Wu H
author_facet	Zhu S Zeng M Feng G Wu H
author_sort	Zhu S
title	Platinum Nanoparticles As A Therapeutic Agent Against Dextran Sodium Sulfate-Induced Colitis In Mice
title_short	Platinum Nanoparticles As A Therapeutic Agent Against Dextran Sodium Sulfate-Induced Colitis In Mice
title_full	Platinum Nanoparticles As A Therapeutic Agent Against Dextran Sodium Sulfate-Induced Colitis In Mice
title_fullStr	Platinum Nanoparticles As A Therapeutic Agent Against Dextran Sodium Sulfate-Induced Colitis In Mice
title_full_unstemmed	Platinum Nanoparticles As A Therapeutic Agent Against Dextran Sodium Sulfate-Induced Colitis In Mice
title_sort	platinum nanoparticles as a therapeutic agent against dextran sodium sulfate-induced colitis in mice
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/34f950b00ca44de7a0f2a134d7e77027
work_keys_str_mv	AT zhus platinumnanoparticlesasatherapeuticagentagainstdextransodiumsulfateinducedcolitisinmice AT zengm platinumnanoparticlesasatherapeuticagentagainstdextransodiumsulfateinducedcolitisinmice AT fengg platinumnanoparticlesasatherapeuticagentagainstdextransodiumsulfateinducedcolitisinmice AT wuh platinumnanoparticlesasatherapeuticagentagainstdextransodiumsulfateinducedcolitisinmice
_version_	1718401480073936896

Platinum Nanoparticles As A Therapeutic Agent Against Dextran Sodium Sulfate-Induced Colitis In Mice

Ejemplares similares