Evaluation of platelet lysate as a substitute for FBS in explant and enzymatic isolation methods of human umbilical cord MSCs

Abstract Mesenchymal stem cells (MSCs) have immense potential for cell-based therapy of acute and chronic pathological conditions. MSC transplantation for cell-based therapy requires a substantial number of cells in the range of 0.5–2.5 × 106 cells/kg body weight of an individual. A prolific source...

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Autores principales:	Sangeetha Kandoi, Praveen kumar L, Bamadeb Patra, Prasanna Vidyasekar, Divya Sivanesan, Vijayalakshmi S., Rajagopal K., Rama Shanker Verma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/34faa0aa839048879168ebc459453cc1
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spelling	oai:doaj.org-article:34faa0aa839048879168ebc459453cc12021-12-02T15:07:51ZEvaluation of platelet lysate as a substitute for FBS in explant and enzymatic isolation methods of human umbilical cord MSCs10.1038/s41598-018-30772-42045-2322https://doaj.org/article/34faa0aa839048879168ebc459453cc12018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30772-4https://doaj.org/toc/2045-2322Abstract Mesenchymal stem cells (MSCs) have immense potential for cell-based therapy of acute and chronic pathological conditions. MSC transplantation for cell-based therapy requires a substantial number of cells in the range of 0.5–2.5 × 106 cells/kg body weight of an individual. A prolific source of MSCs followed by in vitro propagation is therefore an absolute prerequisite for clinical applications. Umbilical cord tissue (UCT) is an abundantly available prolific source of MSC that are fetal in nature and have higher potential for ex-vivo expansion. However, the ex-vivo expansion of MSCs using a xenogeneic supplement such as fetal bovine serum (FBS) carries the risk of transmission of zoonotic infections and immunological reactions. We used platelet lysate (PL) as a xeno-free, allogeneic replacement for FBS and compared the biological and functional characteristics of MSC processed and expanded with PL and FBS by explant and enzymatic method. UCT-MSCs expanded using PL displayed typical immunophenotype, plasticity, immunomodulatory property and chromosomal stability. PL supplementation also showed 2-fold increase in MSC yield from explant culture with improved immunomodulatory activity as compared to enzymatically dissociated cultures. In conclusion, PL from expired platelets is a viable alternative to FBS for generating clinically relevant numbers of MSC from explant cultures over enzymatic method.Sangeetha KandoiPraveen kumar LBamadeb PatraPrasanna VidyasekarDivya SivanesanVijayalakshmi S.Rajagopal K.Rama Shanker VermaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sangeetha Kandoi Praveen kumar L Bamadeb Patra Prasanna Vidyasekar Divya Sivanesan Vijayalakshmi S. Rajagopal K. Rama Shanker Verma Evaluation of platelet lysate as a substitute for FBS in explant and enzymatic isolation methods of human umbilical cord MSCs
description	Abstract Mesenchymal stem cells (MSCs) have immense potential for cell-based therapy of acute and chronic pathological conditions. MSC transplantation for cell-based therapy requires a substantial number of cells in the range of 0.5–2.5 × 106 cells/kg body weight of an individual. A prolific source of MSCs followed by in vitro propagation is therefore an absolute prerequisite for clinical applications. Umbilical cord tissue (UCT) is an abundantly available prolific source of MSC that are fetal in nature and have higher potential for ex-vivo expansion. However, the ex-vivo expansion of MSCs using a xenogeneic supplement such as fetal bovine serum (FBS) carries the risk of transmission of zoonotic infections and immunological reactions. We used platelet lysate (PL) as a xeno-free, allogeneic replacement for FBS and compared the biological and functional characteristics of MSC processed and expanded with PL and FBS by explant and enzymatic method. UCT-MSCs expanded using PL displayed typical immunophenotype, plasticity, immunomodulatory property and chromosomal stability. PL supplementation also showed 2-fold increase in MSC yield from explant culture with improved immunomodulatory activity as compared to enzymatically dissociated cultures. In conclusion, PL from expired platelets is a viable alternative to FBS for generating clinically relevant numbers of MSC from explant cultures over enzymatic method.
format	article
author	Sangeetha Kandoi Praveen kumar L Bamadeb Patra Prasanna Vidyasekar Divya Sivanesan Vijayalakshmi S. Rajagopal K. Rama Shanker Verma
author_facet	Sangeetha Kandoi Praveen kumar L Bamadeb Patra Prasanna Vidyasekar Divya Sivanesan Vijayalakshmi S. Rajagopal K. Rama Shanker Verma
author_sort	Sangeetha Kandoi
title	Evaluation of platelet lysate as a substitute for FBS in explant and enzymatic isolation methods of human umbilical cord MSCs
title_short	Evaluation of platelet lysate as a substitute for FBS in explant and enzymatic isolation methods of human umbilical cord MSCs
title_full	Evaluation of platelet lysate as a substitute for FBS in explant and enzymatic isolation methods of human umbilical cord MSCs
title_fullStr	Evaluation of platelet lysate as a substitute for FBS in explant and enzymatic isolation methods of human umbilical cord MSCs
title_full_unstemmed	Evaluation of platelet lysate as a substitute for FBS in explant and enzymatic isolation methods of human umbilical cord MSCs
title_sort	evaluation of platelet lysate as a substitute for fbs in explant and enzymatic isolation methods of human umbilical cord mscs
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/34faa0aa839048879168ebc459453cc1
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Evaluation of platelet lysate as a substitute for FBS in explant and enzymatic isolation methods of human umbilical cord MSCs

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