Global analysis of differentially expressed genes and proteins in the wheat callus infected by Agrobacterium tumefaciens.

Agrobacterium-mediated plant transformation is an extremely complex and evolved process involving genetic determinants of both the bacteria and the host plant cells. However, the mechanism of the determinants remains obscure, especially in some cereal crops such as wheat, which is recalcitrant for A...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiaohong Zhou, Ke Wang, Dongwen Lv, Chengjun Wu, Jiarui Li, Pei Zhao, Zhishan Lin, Lipu Du, Yueming Yan, Xingguo Ye
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/34fd758c42af4272be75412e2800305c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:34fd758c42af4272be75412e2800305c
record_format	dspace
spelling	oai:doaj.org-article:34fd758c42af4272be75412e2800305c2021-11-18T08:45:34ZGlobal analysis of differentially expressed genes and proteins in the wheat callus infected by Agrobacterium tumefaciens.1932-620310.1371/journal.pone.0079390https://doaj.org/article/34fd758c42af4272be75412e2800305c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24278131/?tool=EBIhttps://doaj.org/toc/1932-6203Agrobacterium-mediated plant transformation is an extremely complex and evolved process involving genetic determinants of both the bacteria and the host plant cells. However, the mechanism of the determinants remains obscure, especially in some cereal crops such as wheat, which is recalcitrant for Agrobacterium-mediated transformation. In this study, differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) were analyzed in wheat callus cells co-cultured with Agrobacterium by using RNA sequencing (RNA-seq) and two-dimensional electrophoresis (2-DE) in conjunction with mass spectrometry (MS). A set of 4,889 DEGs and 90 DEPs were identified, respectively. Most of them are related to metabolism, chromatin assembly or disassembly and immune defense. After comparative analysis, 24 of the 90 DEPs were detected in RNA-seq and proteomics datasets simultaneously. In addition, real-time RT-PCR experiments were performed to check the differential expression of the 24 genes, and the results were consistent with the RNA-seq data. According to gene ontology (GO) analysis, we found that a big part of these differentially expressed genes were related to the process of stress or immunity response. Several putative determinants and candidate effectors responsive to Agrobacterium mediated transformation of wheat cells were discussed. We speculate that some of these genes are possibly related to Agrobacterium infection. Our results will help to understand the interaction between Agrobacterium and host cells, and may facilitate developing efficient transformation strategies in cereal crops.Xiaohong ZhouKe WangDongwen LvChengjun WuJiarui LiPei ZhaoZhishan LinLipu DuYueming YanXingguo YePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79390 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaohong Zhou Ke Wang Dongwen Lv Chengjun Wu Jiarui Li Pei Zhao Zhishan Lin Lipu Du Yueming Yan Xingguo Ye Global analysis of differentially expressed genes and proteins in the wheat callus infected by Agrobacterium tumefaciens.
description	Agrobacterium-mediated plant transformation is an extremely complex and evolved process involving genetic determinants of both the bacteria and the host plant cells. However, the mechanism of the determinants remains obscure, especially in some cereal crops such as wheat, which is recalcitrant for Agrobacterium-mediated transformation. In this study, differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) were analyzed in wheat callus cells co-cultured with Agrobacterium by using RNA sequencing (RNA-seq) and two-dimensional electrophoresis (2-DE) in conjunction with mass spectrometry (MS). A set of 4,889 DEGs and 90 DEPs were identified, respectively. Most of them are related to metabolism, chromatin assembly or disassembly and immune defense. After comparative analysis, 24 of the 90 DEPs were detected in RNA-seq and proteomics datasets simultaneously. In addition, real-time RT-PCR experiments were performed to check the differential expression of the 24 genes, and the results were consistent with the RNA-seq data. According to gene ontology (GO) analysis, we found that a big part of these differentially expressed genes were related to the process of stress or immunity response. Several putative determinants and candidate effectors responsive to Agrobacterium mediated transformation of wheat cells were discussed. We speculate that some of these genes are possibly related to Agrobacterium infection. Our results will help to understand the interaction between Agrobacterium and host cells, and may facilitate developing efficient transformation strategies in cereal crops.
format	article
author	Xiaohong Zhou Ke Wang Dongwen Lv Chengjun Wu Jiarui Li Pei Zhao Zhishan Lin Lipu Du Yueming Yan Xingguo Ye
author_facet	Xiaohong Zhou Ke Wang Dongwen Lv Chengjun Wu Jiarui Li Pei Zhao Zhishan Lin Lipu Du Yueming Yan Xingguo Ye
author_sort	Xiaohong Zhou
title	Global analysis of differentially expressed genes and proteins in the wheat callus infected by Agrobacterium tumefaciens.
title_short	Global analysis of differentially expressed genes and proteins in the wheat callus infected by Agrobacterium tumefaciens.
title_full	Global analysis of differentially expressed genes and proteins in the wheat callus infected by Agrobacterium tumefaciens.
title_fullStr	Global analysis of differentially expressed genes and proteins in the wheat callus infected by Agrobacterium tumefaciens.
title_full_unstemmed	Global analysis of differentially expressed genes and proteins in the wheat callus infected by Agrobacterium tumefaciens.
title_sort	global analysis of differentially expressed genes and proteins in the wheat callus infected by agrobacterium tumefaciens.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/34fd758c42af4272be75412e2800305c
work_keys_str_mv	AT xiaohongzhou globalanalysisofdifferentiallyexpressedgenesandproteinsinthewheatcallusinfectedbyagrobacteriumtumefaciens AT kewang globalanalysisofdifferentiallyexpressedgenesandproteinsinthewheatcallusinfectedbyagrobacteriumtumefaciens AT dongwenlv globalanalysisofdifferentiallyexpressedgenesandproteinsinthewheatcallusinfectedbyagrobacteriumtumefaciens AT chengjunwu globalanalysisofdifferentiallyexpressedgenesandproteinsinthewheatcallusinfectedbyagrobacteriumtumefaciens AT jiaruili globalanalysisofdifferentiallyexpressedgenesandproteinsinthewheatcallusinfectedbyagrobacteriumtumefaciens AT peizhao globalanalysisofdifferentiallyexpressedgenesandproteinsinthewheatcallusinfectedbyagrobacteriumtumefaciens AT zhishanlin globalanalysisofdifferentiallyexpressedgenesandproteinsinthewheatcallusinfectedbyagrobacteriumtumefaciens AT lipudu globalanalysisofdifferentiallyexpressedgenesandproteinsinthewheatcallusinfectedbyagrobacteriumtumefaciens AT yuemingyan globalanalysisofdifferentiallyexpressedgenesandproteinsinthewheatcallusinfectedbyagrobacteriumtumefaciens AT xingguoye globalanalysisofdifferentiallyexpressedgenesandproteinsinthewheatcallusinfectedbyagrobacteriumtumefaciens
_version_	1718421314052554752

Global analysis of differentially expressed genes and proteins in the wheat callus infected by Agrobacterium tumefaciens.

Ejemplares similares