Transcriptional Analysis of Maize Leaf Tissue Treated With Seaweed Extract Under Drought Stress
Kappaphycus alvarezii seaweed extract (KSWE) has been known for its plant biostimulant and stress alleviation activities on various crops. However, very few reports are available depicting its impact at the molecular level, which is crucial in identifying the mechanism of action of KSWE on plants. H...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:35016b420e5c48c9b5f21824c33541742021-12-02T11:16:49ZTranscriptional Analysis of Maize Leaf Tissue Treated With Seaweed Extract Under Drought Stress2571-581X10.3389/fsufs.2021.774978https://doaj.org/article/35016b420e5c48c9b5f21824c33541742021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fsufs.2021.774978/fullhttps://doaj.org/toc/2571-581XKappaphycus alvarezii seaweed extract (KSWE) has been known for its plant biostimulant and stress alleviation activities on various crops. However, very few reports are available depicting its impact at the molecular level, which is crucial in identifying the mechanism of action of KSWE on plants. Here, maize leaf tissue of control and KSWE-treated plants were analyzed for their transcriptional changes under drought stress. KSWE was applied foliarly at the V5 stage of maize crop under drought, and leaf transcriptome analysis was performed. It was found that a total of 380 and 631 genes were up- and downregulated, respectively, due to the application of KSWE. Genes involved in nitrate transportation, signal transmission, photosynthesis, transmembrane transport of various ions, glycogen, and starch biosynthetic processes were found upregulated in KSWE-treated plants, while genes involved in the catabolism of polysaccharide molecules such as starch as well as cell wall macromolecules like chitin and protein degradation were found downregulated. An overview of differentially expressed genes involved in metabolic as well as regulatory processes in KSWE-treated plants was also analyzed via Mapman tool. Phytohormone signaling genes such as cytokinin-independent 1 (involved in cytokine signal transduction), Ent-kaurene synthase and GA20 oxidase (involved in gibberellin synthesis), and gene of 2-oxoglutarate-dependent dioxygenase enzyme activity (involved in ethylene synthesis) were found upregulated while 9-cis-epoxycarotenoid dioxygenase (a gene involved in abscisic acid synthesis) was found downregulated due to the application of KSWE. Modulation of gene expression in maize leaf tissue in response to KSWE treatment elucidates mechanisms to ward off drought stress, which can be extended to understand similar phenomenon in other crops as well. This molecular knowledge can be utilized to make the use of KSWE more efficient and sustainable.Khanjan TrivediKhanjan TrivediVijay Anand K. GopalakrishnanRanjeet KumarRanjeet KumarArup GhoshArup GhoshFrontiers Media S.A.articleKappaphycus alvareziiabiotic stressbiostimulantmaizetranscriptome analysisdroughtNutrition. Foods and food supplyTX341-641Food processing and manufactureTP368-456ENFrontiers in Sustainable Food Systems, Vol 5 (2021) |
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| collection |
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EN |
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Kappaphycus alvarezii abiotic stress biostimulant maize transcriptome analysis drought Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 |
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Kappaphycus alvarezii abiotic stress biostimulant maize transcriptome analysis drought Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 Khanjan Trivedi Khanjan Trivedi Vijay Anand K. Gopalakrishnan Ranjeet Kumar Ranjeet Kumar Arup Ghosh Arup Ghosh Transcriptional Analysis of Maize Leaf Tissue Treated With Seaweed Extract Under Drought Stress |
| description |
Kappaphycus alvarezii seaweed extract (KSWE) has been known for its plant biostimulant and stress alleviation activities on various crops. However, very few reports are available depicting its impact at the molecular level, which is crucial in identifying the mechanism of action of KSWE on plants. Here, maize leaf tissue of control and KSWE-treated plants were analyzed for their transcriptional changes under drought stress. KSWE was applied foliarly at the V5 stage of maize crop under drought, and leaf transcriptome analysis was performed. It was found that a total of 380 and 631 genes were up- and downregulated, respectively, due to the application of KSWE. Genes involved in nitrate transportation, signal transmission, photosynthesis, transmembrane transport of various ions, glycogen, and starch biosynthetic processes were found upregulated in KSWE-treated plants, while genes involved in the catabolism of polysaccharide molecules such as starch as well as cell wall macromolecules like chitin and protein degradation were found downregulated. An overview of differentially expressed genes involved in metabolic as well as regulatory processes in KSWE-treated plants was also analyzed via Mapman tool. Phytohormone signaling genes such as cytokinin-independent 1 (involved in cytokine signal transduction), Ent-kaurene synthase and GA20 oxidase (involved in gibberellin synthesis), and gene of 2-oxoglutarate-dependent dioxygenase enzyme activity (involved in ethylene synthesis) were found upregulated while 9-cis-epoxycarotenoid dioxygenase (a gene involved in abscisic acid synthesis) was found downregulated due to the application of KSWE. Modulation of gene expression in maize leaf tissue in response to KSWE treatment elucidates mechanisms to ward off drought stress, which can be extended to understand similar phenomenon in other crops as well. This molecular knowledge can be utilized to make the use of KSWE more efficient and sustainable. |
| format |
article |
| author |
Khanjan Trivedi Khanjan Trivedi Vijay Anand K. Gopalakrishnan Ranjeet Kumar Ranjeet Kumar Arup Ghosh Arup Ghosh |
| author_facet |
Khanjan Trivedi Khanjan Trivedi Vijay Anand K. Gopalakrishnan Ranjeet Kumar Ranjeet Kumar Arup Ghosh Arup Ghosh |
| author_sort |
Khanjan Trivedi |
| title |
Transcriptional Analysis of Maize Leaf Tissue Treated With Seaweed Extract Under Drought Stress |
| title_short |
Transcriptional Analysis of Maize Leaf Tissue Treated With Seaweed Extract Under Drought Stress |
| title_full |
Transcriptional Analysis of Maize Leaf Tissue Treated With Seaweed Extract Under Drought Stress |
| title_fullStr |
Transcriptional Analysis of Maize Leaf Tissue Treated With Seaweed Extract Under Drought Stress |
| title_full_unstemmed |
Transcriptional Analysis of Maize Leaf Tissue Treated With Seaweed Extract Under Drought Stress |
| title_sort |
transcriptional analysis of maize leaf tissue treated with seaweed extract under drought stress |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/35016b420e5c48c9b5f21824c3354174 |
| work_keys_str_mv |
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| _version_ |
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