Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes

Although advances in protein assembly preparation have provided a new platform for drug delivery during tissue engineering, achieving long-term controlled exosome delivery remains a significant challenge. Diffusion-dominated exosome release using protein hydrogels results in burst release of exosome...

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Autores principales:	Muyang Sun, Qi Li, Huilei Yu, Jin Cheng, Nier Wu, Weili Shi, Fengyuan Zhao, Zhenxing Shao, Qingyang Meng, Haifeng Chen, Xiaoqing Hu, Yingfang Ao
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Exosomes Silk fibroin Cryo-sponge Sustained drug delivery Enzyme-responsive Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3502177ea2cd43e4b9658f3248dcfa45
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id	oai:doaj.org-article:3502177ea2cd43e4b9658f3248dcfa45
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spelling	oai:doaj.org-article:3502177ea2cd43e4b9658f3248dcfa452021-11-04T04:35:29ZCryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes2452-199X10.1016/j.bioactmat.2021.06.017https://doaj.org/article/3502177ea2cd43e4b9658f3248dcfa452022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X21003030https://doaj.org/toc/2452-199XAlthough advances in protein assembly preparation have provided a new platform for drug delivery during tissue engineering, achieving long-term controlled exosome delivery remains a significant challenge. Diffusion-dominated exosome release using protein hydrogels results in burst release of exosomes. Here, a fibroin-based cryo-sponge was developed to provide controlled exosome release. Fibroin chains can self-assemble into silk I structures under ice-cold conditions when annealed above the glass transition temperature. Exosome release is enzyme-responsive, with rates primarily determined by enzymatic degradation of the scaffolds. In vivo experiments have demonstrated that exosomes remain in undigested sponge material for two months, superior to their retention in fibrin glue, a commonly used biomaterial in clinical practice. Fibroin cryo-sponges were implanted subcutaneously in nude mice. The exosome-containing sponge group exhibited better neovascularization and tissue ingrowth effects, demonstrating the efficacy of this exosome-encapsulating strategy by realizing sustained release and maintaining exosome bioactivity. These silk fibroin cryo-sponges containing exosomes provide a new platform for future studies of exosome therapy.Muyang SunQi LiHuilei YuJin ChengNier WuWeili ShiFengyuan ZhaoZhenxing ShaoQingyang MengHaifeng ChenXiaoqing HuYingfang AoKeAi Communications Co., Ltd.articleExosomesSilk fibroinCryo-spongeSustained drug deliveryEnzyme-responsiveMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 8, Iss , Pp 505-514 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Exosomes Silk fibroin Cryo-sponge Sustained drug delivery Enzyme-responsive Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	Exosomes Silk fibroin Cryo-sponge Sustained drug delivery Enzyme-responsive Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Muyang Sun Qi Li Huilei Yu Jin Cheng Nier Wu Weili Shi Fengyuan Zhao Zhenxing Shao Qingyang Meng Haifeng Chen Xiaoqing Hu Yingfang Ao Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes
description	Although advances in protein assembly preparation have provided a new platform for drug delivery during tissue engineering, achieving long-term controlled exosome delivery remains a significant challenge. Diffusion-dominated exosome release using protein hydrogels results in burst release of exosomes. Here, a fibroin-based cryo-sponge was developed to provide controlled exosome release. Fibroin chains can self-assemble into silk I structures under ice-cold conditions when annealed above the glass transition temperature. Exosome release is enzyme-responsive, with rates primarily determined by enzymatic degradation of the scaffolds. In vivo experiments have demonstrated that exosomes remain in undigested sponge material for two months, superior to their retention in fibrin glue, a commonly used biomaterial in clinical practice. Fibroin cryo-sponges were implanted subcutaneously in nude mice. The exosome-containing sponge group exhibited better neovascularization and tissue ingrowth effects, demonstrating the efficacy of this exosome-encapsulating strategy by realizing sustained release and maintaining exosome bioactivity. These silk fibroin cryo-sponges containing exosomes provide a new platform for future studies of exosome therapy.
format	article
author	Muyang Sun Qi Li Huilei Yu Jin Cheng Nier Wu Weili Shi Fengyuan Zhao Zhenxing Shao Qingyang Meng Haifeng Chen Xiaoqing Hu Yingfang Ao
author_facet	Muyang Sun Qi Li Huilei Yu Jin Cheng Nier Wu Weili Shi Fengyuan Zhao Zhenxing Shao Qingyang Meng Haifeng Chen Xiaoqing Hu Yingfang Ao
author_sort	Muyang Sun
title	Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes
title_short	Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes
title_full	Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes
title_fullStr	Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes
title_full_unstemmed	Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes
title_sort	cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/3502177ea2cd43e4b9658f3248dcfa45
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Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes

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