Protocol for scChaRM-seq: Simultaneous profiling of gene expression, DNA methylation, and chromatin accessibility in single cells

Summary: Single-cell multi-omics sequencing technology can infer cell heterogeneity and reveal relationships across molecular layers. Combining single-cell RNA sequencing, DNA methylation, and chromatin accessibility allows a multimodal understanding of cell function and epigenetic regulation within...

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Autores principales:	Rui Yan, Xin Cheng, Fan Guo
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Single Cell Genomics Sequencing RNAseq Molecular Biology Gene Expression Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/350564281b9f4f6894825eac3d8e7538
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spelling	oai:doaj.org-article:350564281b9f4f6894825eac3d8e75382021-11-22T04:30:59ZProtocol for scChaRM-seq: Simultaneous profiling of gene expression, DNA methylation, and chromatin accessibility in single cells2666-166710.1016/j.xpro.2021.100972https://doaj.org/article/350564281b9f4f6894825eac3d8e75382021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S266616672100678Xhttps://doaj.org/toc/2666-1667Summary: Single-cell multi-omics sequencing technology can infer cell heterogeneity and reveal relationships across molecular layers. Combining single-cell RNA sequencing, DNA methylation, and chromatin accessibility allows a multimodal understanding of cell function and epigenetic regulation within individual cells. Here, we offer a protocol to perform scChaRM-seq (single-cell chromatin accessibility, RNA barcoding, and DNA methylation sequencing), which has been applied to study de novo DNA methylation and its relationship with transcription and chromatin accessibility in single human oocytes.For complete details on the use and execution of this protocol, please refer to Yan et al. (2021).Rui YanXin ChengFan GuoElsevierarticleSingle CellGenomicsSequencingRNAseqMolecular BiologyGene ExpressionScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100972- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Single Cell Genomics Sequencing RNAseq Molecular Biology Gene Expression Science (General) Q1-390
spellingShingle	Single Cell Genomics Sequencing RNAseq Molecular Biology Gene Expression Science (General) Q1-390 Rui Yan Xin Cheng Fan Guo Protocol for scChaRM-seq: Simultaneous profiling of gene expression, DNA methylation, and chromatin accessibility in single cells
description	Summary: Single-cell multi-omics sequencing technology can infer cell heterogeneity and reveal relationships across molecular layers. Combining single-cell RNA sequencing, DNA methylation, and chromatin accessibility allows a multimodal understanding of cell function and epigenetic regulation within individual cells. Here, we offer a protocol to perform scChaRM-seq (single-cell chromatin accessibility, RNA barcoding, and DNA methylation sequencing), which has been applied to study de novo DNA methylation and its relationship with transcription and chromatin accessibility in single human oocytes.For complete details on the use and execution of this protocol, please refer to Yan et al. (2021).
format	article
author	Rui Yan Xin Cheng Fan Guo
author_facet	Rui Yan Xin Cheng Fan Guo
author_sort	Rui Yan
title	Protocol for scChaRM-seq: Simultaneous profiling of gene expression, DNA methylation, and chromatin accessibility in single cells
title_short	Protocol for scChaRM-seq: Simultaneous profiling of gene expression, DNA methylation, and chromatin accessibility in single cells
title_full	Protocol for scChaRM-seq: Simultaneous profiling of gene expression, DNA methylation, and chromatin accessibility in single cells
title_fullStr	Protocol for scChaRM-seq: Simultaneous profiling of gene expression, DNA methylation, and chromatin accessibility in single cells
title_full_unstemmed	Protocol for scChaRM-seq: Simultaneous profiling of gene expression, DNA methylation, and chromatin accessibility in single cells
title_sort	protocol for sccharm-seq: simultaneous profiling of gene expression, dna methylation, and chromatin accessibility in single cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/350564281b9f4f6894825eac3d8e7538
work_keys_str_mv	AT ruiyan protocolforsccharmseqsimultaneousprofilingofgeneexpressiondnamethylationandchromatinaccessibilityinsinglecells AT xincheng protocolforsccharmseqsimultaneousprofilingofgeneexpressiondnamethylationandchromatinaccessibilityinsinglecells AT fanguo protocolforsccharmseqsimultaneousprofilingofgeneexpressiondnamethylationandchromatinaccessibilityinsinglecells
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Protocol for scChaRM-seq: Simultaneous profiling of gene expression, DNA methylation, and chromatin accessibility in single cells

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