Temporal dynamics of intradermal cytokine response to tuberculin in Mycobacterium bovis BCG-vaccinated cattle using sampling microneedles

Abstract Bovine tuberculosis (bTB) is a disease of livestock with severe and worldwide economic, animal welfare and zoonotic consequences. Application of test-and-slaughter-based control polices reliant on tuberculin skin testing has been the mainstay of bTB control in cattle. However, little is kno...

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Autores principales:	Sabine Steinbach, Sasan Jalili-Firoozinezhad, Sreenidhi Srinivasan, Mariane B. Melo, Sonya Middleton, Timm Konold, Michael Coad, Paula T. Hammond, Darrell J. Irvine, Martin Vordermeier, Vivek Kapur
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3508ccbc973349b3af867acbcdaae274
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spelling	oai:doaj.org-article:3508ccbc973349b3af867acbcdaae2742021-12-02T13:27:04ZTemporal dynamics of intradermal cytokine response to tuberculin in Mycobacterium bovis BCG-vaccinated cattle using sampling microneedles10.1038/s41598-021-86398-62045-2322https://doaj.org/article/3508ccbc973349b3af867acbcdaae2742021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86398-6https://doaj.org/toc/2045-2322Abstract Bovine tuberculosis (bTB) is a disease of livestock with severe and worldwide economic, animal welfare and zoonotic consequences. Application of test-and-slaughter-based control polices reliant on tuberculin skin testing has been the mainstay of bTB control in cattle. However, little is known about the temporal development of the bovine tuberculin skin test response at the dermal sites of antigen injection. To fill this knowledge gap, we applied minimally-invasive sampling microneedles (SMNs) for intradermal sampling of interstitial fluid at the tuberculin skin test sites in Mycobacterium bovis BCG-vaccinated calves and determined the temporal dynamics of a panel of 15 cytokines and chemokines in situ and in the peripheral blood. The results reveal an orchestrated and coordinated cytokine and local chemokine response, identified IL-1RA as a potential soluble biomarker of a positive tuberculin skin response, and confirmed the utility of IFN-γ and IP-10 for bTB detection in blood-based assays. Together, the results highlight the utility of SMNs to identify novel biomarkers and provide mechanistic insights on the intradermal cytokine and chemokine responses associated with the tuberculin skin test in BCG-sensitized cattle.Sabine SteinbachSasan Jalili-FiroozinezhadSreenidhi SrinivasanMariane B. MeloSonya MiddletonTimm KonoldMichael CoadPaula T. HammondDarrell J. IrvineMartin VordermeierVivek KapurNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sabine Steinbach Sasan Jalili-Firoozinezhad Sreenidhi Srinivasan Mariane B. Melo Sonya Middleton Timm Konold Michael Coad Paula T. Hammond Darrell J. Irvine Martin Vordermeier Vivek Kapur Temporal dynamics of intradermal cytokine response to tuberculin in Mycobacterium bovis BCG-vaccinated cattle using sampling microneedles
description	Abstract Bovine tuberculosis (bTB) is a disease of livestock with severe and worldwide economic, animal welfare and zoonotic consequences. Application of test-and-slaughter-based control polices reliant on tuberculin skin testing has been the mainstay of bTB control in cattle. However, little is known about the temporal development of the bovine tuberculin skin test response at the dermal sites of antigen injection. To fill this knowledge gap, we applied minimally-invasive sampling microneedles (SMNs) for intradermal sampling of interstitial fluid at the tuberculin skin test sites in Mycobacterium bovis BCG-vaccinated calves and determined the temporal dynamics of a panel of 15 cytokines and chemokines in situ and in the peripheral blood. The results reveal an orchestrated and coordinated cytokine and local chemokine response, identified IL-1RA as a potential soluble biomarker of a positive tuberculin skin response, and confirmed the utility of IFN-γ and IP-10 for bTB detection in blood-based assays. Together, the results highlight the utility of SMNs to identify novel biomarkers and provide mechanistic insights on the intradermal cytokine and chemokine responses associated with the tuberculin skin test in BCG-sensitized cattle.
format	article
author	Sabine Steinbach Sasan Jalili-Firoozinezhad Sreenidhi Srinivasan Mariane B. Melo Sonya Middleton Timm Konold Michael Coad Paula T. Hammond Darrell J. Irvine Martin Vordermeier Vivek Kapur
author_facet	Sabine Steinbach Sasan Jalili-Firoozinezhad Sreenidhi Srinivasan Mariane B. Melo Sonya Middleton Timm Konold Michael Coad Paula T. Hammond Darrell J. Irvine Martin Vordermeier Vivek Kapur
author_sort	Sabine Steinbach
title	Temporal dynamics of intradermal cytokine response to tuberculin in Mycobacterium bovis BCG-vaccinated cattle using sampling microneedles
title_short	Temporal dynamics of intradermal cytokine response to tuberculin in Mycobacterium bovis BCG-vaccinated cattle using sampling microneedles
title_full	Temporal dynamics of intradermal cytokine response to tuberculin in Mycobacterium bovis BCG-vaccinated cattle using sampling microneedles
title_fullStr	Temporal dynamics of intradermal cytokine response to tuberculin in Mycobacterium bovis BCG-vaccinated cattle using sampling microneedles
title_full_unstemmed	Temporal dynamics of intradermal cytokine response to tuberculin in Mycobacterium bovis BCG-vaccinated cattle using sampling microneedles
title_sort	temporal dynamics of intradermal cytokine response to tuberculin in mycobacterium bovis bcg-vaccinated cattle using sampling microneedles
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3508ccbc973349b3af867acbcdaae274
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Temporal dynamics of intradermal cytokine response to tuberculin in Mycobacterium bovis BCG-vaccinated cattle using sampling microneedles

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