Structural and developmental expression of Ss-riok-2, an RIO protein kinase encoding gene of Strongyloides stercoralis

Abstract RIO kinases are essential atypical protein kinases in diverse prokaryotic and eukaryotic organisms, playing significant roles in yeast and humans. However, little is known about their functions in parasitic nematodes. In the present study, we have isolated and characterized the full-length...

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Autores principales:	Wei-Qiang Lei, James B. Lok, Wang Yuan, Yue-Zhou Zhang, Jonathan D. Stoltzfus, Robin B. Gasser, Si-Yuan He, Huan Zhou, Rui Zhou, Jun-Long Zhao, Min Hu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/35097fbd72704ccdb5011348d86a5e1f
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spelling	oai:doaj.org-article:35097fbd72704ccdb5011348d86a5e1f2021-12-02T16:07:03ZStructural and developmental expression of Ss-riok-2, an RIO protein kinase encoding gene of Strongyloides stercoralis10.1038/s41598-017-07991-22045-2322https://doaj.org/article/35097fbd72704ccdb5011348d86a5e1f2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07991-2https://doaj.org/toc/2045-2322Abstract RIO kinases are essential atypical protein kinases in diverse prokaryotic and eukaryotic organisms, playing significant roles in yeast and humans. However, little is known about their functions in parasitic nematodes. In the present study, we have isolated and characterized the full-length cDNA, gDNA and a putative promoter of a RIOK-2 protein kinase (Ss-RIOK-2) encoding gene (Ss-riok-2) from Strongyloides stercoralis, a medically important parasitic nematode (Order Rhabditida). A three-dimensional structure (3D) model of Ss-RIOK-2 was generated using the Chaetomium thermophilum RIOK-2 protein kinase (Ct-RIOK-2) crystal structure 4GYG as a template. A docking study revealed some critical sites for ATP binding and metal binding. The putative promoter of Ss-riok-2 contains a number of conserved elements. RNAseq analysis revealed the highest levels of the Ss-riok-2 transcript in free-living females and parasitic females. To identify anatomical patterns of Ss-riok-2 expression in S. stercoralis, we observed expression patterns of a transgene construct encoding green fluorescent protein under the Ss-riok-2 promoter in post free-living S. stercoralis. Expression driven by this promoter predominated in intestinal cells. This study demonstrates significant advancement in molecular and cellular biological study of S. stercoralis and of parasitic nematodes generally, and provides a foundation for further functional genomic studies.Wei-Qiang LeiJames B. LokWang YuanYue-Zhou ZhangJonathan D. StoltzfusRobin B. GasserSi-Yuan HeHuan ZhouRui ZhouJun-Long ZhaoMin HuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei-Qiang Lei James B. Lok Wang Yuan Yue-Zhou Zhang Jonathan D. Stoltzfus Robin B. Gasser Si-Yuan He Huan Zhou Rui Zhou Jun-Long Zhao Min Hu Structural and developmental expression of Ss-riok-2, an RIO protein kinase encoding gene of Strongyloides stercoralis
description	Abstract RIO kinases are essential atypical protein kinases in diverse prokaryotic and eukaryotic organisms, playing significant roles in yeast and humans. However, little is known about their functions in parasitic nematodes. In the present study, we have isolated and characterized the full-length cDNA, gDNA and a putative promoter of a RIOK-2 protein kinase (Ss-RIOK-2) encoding gene (Ss-riok-2) from Strongyloides stercoralis, a medically important parasitic nematode (Order Rhabditida). A three-dimensional structure (3D) model of Ss-RIOK-2 was generated using the Chaetomium thermophilum RIOK-2 protein kinase (Ct-RIOK-2) crystal structure 4GYG as a template. A docking study revealed some critical sites for ATP binding and metal binding. The putative promoter of Ss-riok-2 contains a number of conserved elements. RNAseq analysis revealed the highest levels of the Ss-riok-2 transcript in free-living females and parasitic females. To identify anatomical patterns of Ss-riok-2 expression in S. stercoralis, we observed expression patterns of a transgene construct encoding green fluorescent protein under the Ss-riok-2 promoter in post free-living S. stercoralis. Expression driven by this promoter predominated in intestinal cells. This study demonstrates significant advancement in molecular and cellular biological study of S. stercoralis and of parasitic nematodes generally, and provides a foundation for further functional genomic studies.
format	article
author	Wei-Qiang Lei James B. Lok Wang Yuan Yue-Zhou Zhang Jonathan D. Stoltzfus Robin B. Gasser Si-Yuan He Huan Zhou Rui Zhou Jun-Long Zhao Min Hu
author_facet	Wei-Qiang Lei James B. Lok Wang Yuan Yue-Zhou Zhang Jonathan D. Stoltzfus Robin B. Gasser Si-Yuan He Huan Zhou Rui Zhou Jun-Long Zhao Min Hu
author_sort	Wei-Qiang Lei
title	Structural and developmental expression of Ss-riok-2, an RIO protein kinase encoding gene of Strongyloides stercoralis
title_short	Structural and developmental expression of Ss-riok-2, an RIO protein kinase encoding gene of Strongyloides stercoralis
title_full	Structural and developmental expression of Ss-riok-2, an RIO protein kinase encoding gene of Strongyloides stercoralis
title_fullStr	Structural and developmental expression of Ss-riok-2, an RIO protein kinase encoding gene of Strongyloides stercoralis
title_full_unstemmed	Structural and developmental expression of Ss-riok-2, an RIO protein kinase encoding gene of Strongyloides stercoralis
title_sort	structural and developmental expression of ss-riok-2, an rio protein kinase encoding gene of strongyloides stercoralis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/35097fbd72704ccdb5011348d86a5e1f
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Structural and developmental expression of Ss-riok-2, an RIO protein kinase encoding gene of Strongyloides stercoralis

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