CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1

Abstract More than 70% of colorectal, prostate, ovarian, pancreatic and breast cancer specimens show expression of CD276 (B7–H3), a potential immune checkpoint family member. Several studies have shown that high CD276 expression in cancer cells correlates with a poor clinical prognosis. This has bee...

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Autores principales:	Sibel Durlanik, Katrin Fundel-Clemens, Coralie Viollet, Heinrich J. Huber, Martin Lenter, Kerstin Kitt, Stefan Pflanz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3514e95bf4724fdea484cf4f1f37996c
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spelling	oai:doaj.org-article:3514e95bf4724fdea484cf4f1f37996c2021-12-02T17:57:16ZCD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-110.1038/s41598-021-94360-92045-2322https://doaj.org/article/3514e95bf4724fdea484cf4f1f37996c2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94360-9https://doaj.org/toc/2045-2322Abstract More than 70% of colorectal, prostate, ovarian, pancreatic and breast cancer specimens show expression of CD276 (B7–H3), a potential immune checkpoint family member. Several studies have shown that high CD276 expression in cancer cells correlates with a poor clinical prognosis. This has been associated with the presence of lower tumor infiltrating leukocytes. Among those, tumor-associated macrophages can comprise up to 50% of the tumor mass and are thought to support tumor growth through various mechanisms. However, a lack of information on CD276 function and interaction partner(s) impedes rigorous evaluation of CD276 as a therapeutic target in oncology. Therefore, we aimed to understand the relevance of CD276 in tumor-macrophage interaction by employing a 3D spheroid coculture system with human cells. Our data show a role for tumor-expressed CD276 on the macrophage recruitment into the tumor spheroid, and also in regulation of the extracellular matrix modulator PAI-1. Furthermore, our experiments focusing on macrophage-expressed CD276 suggest that the antibody-dependent CD276 engagement triggers predominantly inhibitory signaling networks in human macrophages.Sibel DurlanikKatrin Fundel-ClemensCoralie ViolletHeinrich J. HuberMartin LenterKerstin KittStefan PflanzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sibel Durlanik Katrin Fundel-Clemens Coralie Viollet Heinrich J. Huber Martin Lenter Kerstin Kitt Stefan Pflanz CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1
description	Abstract More than 70% of colorectal, prostate, ovarian, pancreatic and breast cancer specimens show expression of CD276 (B7–H3), a potential immune checkpoint family member. Several studies have shown that high CD276 expression in cancer cells correlates with a poor clinical prognosis. This has been associated with the presence of lower tumor infiltrating leukocytes. Among those, tumor-associated macrophages can comprise up to 50% of the tumor mass and are thought to support tumor growth through various mechanisms. However, a lack of information on CD276 function and interaction partner(s) impedes rigorous evaluation of CD276 as a therapeutic target in oncology. Therefore, we aimed to understand the relevance of CD276 in tumor-macrophage interaction by employing a 3D spheroid coculture system with human cells. Our data show a role for tumor-expressed CD276 on the macrophage recruitment into the tumor spheroid, and also in regulation of the extracellular matrix modulator PAI-1. Furthermore, our experiments focusing on macrophage-expressed CD276 suggest that the antibody-dependent CD276 engagement triggers predominantly inhibitory signaling networks in human macrophages.
format	article
author	Sibel Durlanik Katrin Fundel-Clemens Coralie Viollet Heinrich J. Huber Martin Lenter Kerstin Kitt Stefan Pflanz
author_facet	Sibel Durlanik Katrin Fundel-Clemens Coralie Viollet Heinrich J. Huber Martin Lenter Kerstin Kitt Stefan Pflanz
author_sort	Sibel Durlanik
title	CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1
title_short	CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1
title_full	CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1
title_fullStr	CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1
title_full_unstemmed	CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1
title_sort	cd276 is an important player in macrophage recruitment into the tumor and an upstream regulator for pai-1
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3514e95bf4724fdea484cf4f1f37996c
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CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1

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