Establishment and Validation of a GC–MS/MS Method for the Quantification of Penicillin G Residues in Poultry Eggs

A simple and sensitive gas chromatography–tandem mass spectrometry (GC–MS/MS) method was established for the quantitative screening of penicillin G residues in chicken and duck eggs (whole egg, yolk and albumen). The analyte was separated on a TG-1MS capillary column (30.0 m × 0.25 mm i.d., 0.25 μm)...

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Autores principales:	Chujun Liu, Yawen Guo, Bo Wang, Lan Chen, Kaizhou Xie, Chenggen Yang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	penicillin G GC–MS/MS ASE poultry eggs SPE Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/3515c6e968ed4c0c8d46baed42467299
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spelling	oai:doaj.org-article:3515c6e968ed4c0c8d46baed424672992021-11-25T17:34:58ZEstablishment and Validation of a GC–MS/MS Method for the Quantification of Penicillin G Residues in Poultry Eggs10.3390/foods101127352304-8158https://doaj.org/article/3515c6e968ed4c0c8d46baed424672992021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2735https://doaj.org/toc/2304-8158A simple and sensitive gas chromatography–tandem mass spectrometry (GC–MS/MS) method was established for the quantitative screening of penicillin G residues in chicken and duck eggs (whole egg, yolk and albumen). The analyte was separated on a TG-1MS capillary column (30.0 m × 0.25 mm i.d., 0.25 μm) with an external calibration method and electron impact (EI) ionization. Samples were pretreated using an accelerated solvent extraction (ASE) procedure followed by solid-phase extraction (SPE) on HLB cartridges (60 mg/3 mL). The derivative, which was safer and easier to store than penicillin G, was obtained by reacting trimethylsilyl diazomethane (TMSD) with penicillin G. The method was validated by the following parameters: linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ). The matrix-matched calibration curves had good linearity (R2 ≥ 0.9994) within the concentration range of LOQ–200.0 µg/kg for penicillin G in the sample matrices. In the same concentration range, the accuracy, in terms of recovery, was 80.31–94.50%; the relative standard deviation (RSD), intra-day RSD and inter-day RSD ranged from 1.24 to 3.44%, 2.13 to 4.82% and 2.74 to 6.13%, respectively. The LODs and LOQs of penicillin G in the matrices were in the ranges of 1.70–3.20 and 6.10–8.50 μg/kg, respectively. The applicability of the GC–MS/MS method was demonstrated by the determination of poultry eggs obtained from local markets with no penicillin G residues.Chujun LiuYawen GuoBo WangLan ChenKaizhou XieChenggen YangMDPI AGarticlepenicillin GGC–MS/MSASEpoultry eggsSPEChemical technologyTP1-1185ENFoods, Vol 10, Iss 2735, p 2735 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	penicillin G GC–MS/MS ASE poultry eggs SPE Chemical technology TP1-1185
spellingShingle	penicillin G GC–MS/MS ASE poultry eggs SPE Chemical technology TP1-1185 Chujun Liu Yawen Guo Bo Wang Lan Chen Kaizhou Xie Chenggen Yang Establishment and Validation of a GC–MS/MS Method for the Quantification of Penicillin G Residues in Poultry Eggs
description	A simple and sensitive gas chromatography–tandem mass spectrometry (GC–MS/MS) method was established for the quantitative screening of penicillin G residues in chicken and duck eggs (whole egg, yolk and albumen). The analyte was separated on a TG-1MS capillary column (30.0 m × 0.25 mm i.d., 0.25 μm) with an external calibration method and electron impact (EI) ionization. Samples were pretreated using an accelerated solvent extraction (ASE) procedure followed by solid-phase extraction (SPE) on HLB cartridges (60 mg/3 mL). The derivative, which was safer and easier to store than penicillin G, was obtained by reacting trimethylsilyl diazomethane (TMSD) with penicillin G. The method was validated by the following parameters: linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ). The matrix-matched calibration curves had good linearity (R2 ≥ 0.9994) within the concentration range of LOQ–200.0 µg/kg for penicillin G in the sample matrices. In the same concentration range, the accuracy, in terms of recovery, was 80.31–94.50%; the relative standard deviation (RSD), intra-day RSD and inter-day RSD ranged from 1.24 to 3.44%, 2.13 to 4.82% and 2.74 to 6.13%, respectively. The LODs and LOQs of penicillin G in the matrices were in the ranges of 1.70–3.20 and 6.10–8.50 μg/kg, respectively. The applicability of the GC–MS/MS method was demonstrated by the determination of poultry eggs obtained from local markets with no penicillin G residues.
format	article
author	Chujun Liu Yawen Guo Bo Wang Lan Chen Kaizhou Xie Chenggen Yang
author_facet	Chujun Liu Yawen Guo Bo Wang Lan Chen Kaizhou Xie Chenggen Yang
author_sort	Chujun Liu
title	Establishment and Validation of a GC–MS/MS Method for the Quantification of Penicillin G Residues in Poultry Eggs
title_short	Establishment and Validation of a GC–MS/MS Method for the Quantification of Penicillin G Residues in Poultry Eggs
title_full	Establishment and Validation of a GC–MS/MS Method for the Quantification of Penicillin G Residues in Poultry Eggs
title_fullStr	Establishment and Validation of a GC–MS/MS Method for the Quantification of Penicillin G Residues in Poultry Eggs
title_full_unstemmed	Establishment and Validation of a GC–MS/MS Method for the Quantification of Penicillin G Residues in Poultry Eggs
title_sort	establishment and validation of a gc–ms/ms method for the quantification of penicillin g residues in poultry eggs
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3515c6e968ed4c0c8d46baed42467299
work_keys_str_mv	AT chujunliu establishmentandvalidationofagcmsmsmethodforthequantificationofpenicillingresiduesinpoultryeggs AT yawenguo establishmentandvalidationofagcmsmsmethodforthequantificationofpenicillingresiduesinpoultryeggs AT bowang establishmentandvalidationofagcmsmsmethodforthequantificationofpenicillingresiduesinpoultryeggs AT lanchen establishmentandvalidationofagcmsmsmethodforthequantificationofpenicillingresiduesinpoultryeggs AT kaizhouxie establishmentandvalidationofagcmsmsmethodforthequantificationofpenicillingresiduesinpoultryeggs AT chenggenyang establishmentandvalidationofagcmsmsmethodforthequantificationofpenicillingresiduesinpoultryeggs
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Establishment and Validation of a GC–MS/MS Method for the Quantification of Penicillin G Residues in Poultry Eggs

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