Carbon nanospheres mediated delivery of nuclear matrix protein SMAR1 to direct experimental autoimmune encephalomyelitis in mice

Sijo V Chemmannur,1,* Prasad Bhagat,2,* Bhalchandra Mirlekar,1 Kishore M Paknikar,2 Samit Chattopadhyay1,3 1Disease and Chromatin Biology Laboratory, National Center for Cell Science, Pune University Campus, Pune, Maharashtra, India; 2Center for Nanobioscience, Agharkar Research Institute, Pune, Ma...

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Autores principales:	Chemmannur SV, Bhagat P, Mirlekar B, Paknikar KM, Chattopadhyay S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Carbon nanospheres EAE IL-17 SMAR1 Th17 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3515e8cad2274d7e8f242e38f9d4899a
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spelling	oai:doaj.org-article:3515e8cad2274d7e8f242e38f9d4899a2021-12-02T05:04:24ZCarbon nanospheres mediated delivery of nuclear matrix protein SMAR1 to direct experimental autoimmune encephalomyelitis in mice1178-2013https://doaj.org/article/3515e8cad2274d7e8f242e38f9d4899a2016-05-01T00:00:00Zhttps://www.dovepress.com/carbon-nanospheres-mediated-delivery-of-nuclear-matrix-protein-smar1-t-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sijo V Chemmannur,1,* Prasad Bhagat,2,* Bhalchandra Mirlekar,1 Kishore M Paknikar,2 Samit Chattopadhyay1,3 1Disease and Chromatin Biology Laboratory, National Center for Cell Science, Pune University Campus, Pune, Maharashtra, India; 2Center for Nanobioscience, Agharkar Research Institute, Pune, Maharashtra, India; 3Indian Institute of Chemical Biology, Kolkata, India *These authors have contributed equally to this work Abstract: Owing to the suppression of immune responses and associated side effects, steroid based treatments for inflammatory encephalitis disease can be detrimental. Here, we demonstrate a novel carbon nanosphere (CNP) based treatment regime for encephalomyelitis in mice by exploiting the functional property of the nuclear matrix binding protein SMAR1. A truncated part of SMAR1 ie, the DNA binding domain was conjugated with hydrothermally synthesized CNPs. When administered intravenously, the conjugate suppressed experimental animal encephalomyelitis in T cell specific conditional SMAR1 knockout mice (SMAR-/-). Further, CNP-SMAR1 conjugate delayed the onset of the disease and reduced the demyelination significantly. There was a significant decrease in the production of IL-17 after re-stimulation with MOG. Altogether, our findings suggest a potential carbon nanomaterial based therapeutic intervention to combat Th17 mediated autoimmune diseases including experimental autoimmune encephalomyelitis. Keywords: carbon nanospheres, EAE, IL-17, SMAR1, Th17Chemmannur SVBhagat PMirlekar BPaknikar KMChattopadhyay SDove Medical PressarticleCarbon nanospheresEAEIL-17SMAR1Th17Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 2039-2051 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Carbon nanospheres EAE IL-17 SMAR1 Th17 Medicine (General) R5-920
spellingShingle	Carbon nanospheres EAE IL-17 SMAR1 Th17 Medicine (General) R5-920 Chemmannur SV Bhagat P Mirlekar B Paknikar KM Chattopadhyay S Carbon nanospheres mediated delivery of nuclear matrix protein SMAR1 to direct experimental autoimmune encephalomyelitis in mice
description	Sijo V Chemmannur,1,* Prasad Bhagat,2,* Bhalchandra Mirlekar,1 Kishore M Paknikar,2 Samit Chattopadhyay1,3 1Disease and Chromatin Biology Laboratory, National Center for Cell Science, Pune University Campus, Pune, Maharashtra, India; 2Center for Nanobioscience, Agharkar Research Institute, Pune, Maharashtra, India; 3Indian Institute of Chemical Biology, Kolkata, India *These authors have contributed equally to this work Abstract: Owing to the suppression of immune responses and associated side effects, steroid based treatments for inflammatory encephalitis disease can be detrimental. Here, we demonstrate a novel carbon nanosphere (CNP) based treatment regime for encephalomyelitis in mice by exploiting the functional property of the nuclear matrix binding protein SMAR1. A truncated part of SMAR1 ie, the DNA binding domain was conjugated with hydrothermally synthesized CNPs. When administered intravenously, the conjugate suppressed experimental animal encephalomyelitis in T cell specific conditional SMAR1 knockout mice (SMAR-/-). Further, CNP-SMAR1 conjugate delayed the onset of the disease and reduced the demyelination significantly. There was a significant decrease in the production of IL-17 after re-stimulation with MOG. Altogether, our findings suggest a potential carbon nanomaterial based therapeutic intervention to combat Th17 mediated autoimmune diseases including experimental autoimmune encephalomyelitis. Keywords: carbon nanospheres, EAE, IL-17, SMAR1, Th17
format	article
author	Chemmannur SV Bhagat P Mirlekar B Paknikar KM Chattopadhyay S
author_facet	Chemmannur SV Bhagat P Mirlekar B Paknikar KM Chattopadhyay S
author_sort	Chemmannur SV
title	Carbon nanospheres mediated delivery of nuclear matrix protein SMAR1 to direct experimental autoimmune encephalomyelitis in mice
title_short	Carbon nanospheres mediated delivery of nuclear matrix protein SMAR1 to direct experimental autoimmune encephalomyelitis in mice
title_full	Carbon nanospheres mediated delivery of nuclear matrix protein SMAR1 to direct experimental autoimmune encephalomyelitis in mice
title_fullStr	Carbon nanospheres mediated delivery of nuclear matrix protein SMAR1 to direct experimental autoimmune encephalomyelitis in mice
title_full_unstemmed	Carbon nanospheres mediated delivery of nuclear matrix protein SMAR1 to direct experimental autoimmune encephalomyelitis in mice
title_sort	carbon nanospheres mediated delivery of nuclear matrix protein smar1 to direct experimental autoimmune encephalomyelitis in mice
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/3515e8cad2274d7e8f242e38f9d4899a
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_version_	1718400642588868608

Carbon nanospheres mediated delivery of nuclear matrix protein SMAR1 to direct experimental autoimmune encephalomyelitis in mice

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