Hydrogen Promotes the M1 Macrophage Conversion During the Polarization of Macrophages in Necrotizing Enterocolitis

Background: Hydrogen is protective against intestinal injury in necrotizing enterocolitis (NEC), mainly through to alleviate inflammation response. The M1 macrophages can promote inflammation. We hypothesized that hydrogen would promote the M1 macrophages conversion during the polarization and reduc...

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Autores principales:	Shenghua Yu, ZhiBao Lv, Zhimei Gao, Jingyi Shi, Qingfeng Sheng, Lulu Zheng, Junmei Zhou, Xueli Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	necrotizing enterocolitis macrophage polarization hydrogen molecule NF-κB mice Pediatrics RJ1-570
Acceso en línea:	https://doaj.org/article/351c408728f24e9b980f00f9dcdb02eb
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spelling	oai:doaj.org-article:351c408728f24e9b980f00f9dcdb02eb2021-11-17T05:35:30ZHydrogen Promotes the M1 Macrophage Conversion During the Polarization of Macrophages in Necrotizing Enterocolitis2296-236010.3389/fped.2021.710382https://doaj.org/article/351c408728f24e9b980f00f9dcdb02eb2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fped.2021.710382/fullhttps://doaj.org/toc/2296-2360Background: Hydrogen is protective against intestinal injury in necrotizing enterocolitis (NEC), mainly through to alleviate inflammation response. The M1 macrophages can promote inflammation. We hypothesized that hydrogen would promote the M1 macrophages conversion during the polarization and reduce the inflammatory factors in NEC.Methods: We used M1 and M2 macrophages induced from RAW264.7 cells and bone marrow-derived macrophages, models of NEC and macrophages derived from spleens, abdominal lymph nodes and lamina propria in model mice. Cytokines, CD16/32 and CD206 were measured by quantitative PCR, flow cytometry. Nuclear factor-κB (NF-κB) p65 were determined by western blot. Histology staining were used to assess the severity of NEC.Results: Macrophages were successfully polarized to M1 or M2 by assessing the expression of inflammatory factors. Pro-inflammatory factors and CD16/32 in M1 macrophages were decreased, and the expression of CD16/32 in lamina propria were inhibited after treatment with hydrogen, but the changes has no effects in other tissues. Hydrogen inhibited the NF-κB p65 in M1 macrophages nucleus and distal ileum of NEC. HE staining showed hydrogen could attenuate the severity of NEC.Conclusion: Hydrogen could attenuate the severity of NEC through promoting M1 macrophages conversion by inhibited the expression of NF-κB p65 in the nucleus.Shenghua YuZhiBao LvZhimei GaoJingyi ShiQingfeng ShengLulu ZhengJunmei ZhouXueli WangFrontiers Media S.A.articlenecrotizing enterocolitismacrophage polarizationhydrogen moleculeNF-κBmicePediatricsRJ1-570ENFrontiers in Pediatrics, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	necrotizing enterocolitis macrophage polarization hydrogen molecule NF-κB mice Pediatrics RJ1-570
spellingShingle	necrotizing enterocolitis macrophage polarization hydrogen molecule NF-κB mice Pediatrics RJ1-570 Shenghua Yu ZhiBao Lv Zhimei Gao Jingyi Shi Qingfeng Sheng Lulu Zheng Junmei Zhou Xueli Wang Hydrogen Promotes the M1 Macrophage Conversion During the Polarization of Macrophages in Necrotizing Enterocolitis
description	Background: Hydrogen is protective against intestinal injury in necrotizing enterocolitis (NEC), mainly through to alleviate inflammation response. The M1 macrophages can promote inflammation. We hypothesized that hydrogen would promote the M1 macrophages conversion during the polarization and reduce the inflammatory factors in NEC.Methods: We used M1 and M2 macrophages induced from RAW264.7 cells and bone marrow-derived macrophages, models of NEC and macrophages derived from spleens, abdominal lymph nodes and lamina propria in model mice. Cytokines, CD16/32 and CD206 were measured by quantitative PCR, flow cytometry. Nuclear factor-κB (NF-κB) p65 were determined by western blot. Histology staining were used to assess the severity of NEC.Results: Macrophages were successfully polarized to M1 or M2 by assessing the expression of inflammatory factors. Pro-inflammatory factors and CD16/32 in M1 macrophages were decreased, and the expression of CD16/32 in lamina propria were inhibited after treatment with hydrogen, but the changes has no effects in other tissues. Hydrogen inhibited the NF-κB p65 in M1 macrophages nucleus and distal ileum of NEC. HE staining showed hydrogen could attenuate the severity of NEC.Conclusion: Hydrogen could attenuate the severity of NEC through promoting M1 macrophages conversion by inhibited the expression of NF-κB p65 in the nucleus.
format	article
author	Shenghua Yu ZhiBao Lv Zhimei Gao Jingyi Shi Qingfeng Sheng Lulu Zheng Junmei Zhou Xueli Wang
author_facet	Shenghua Yu ZhiBao Lv Zhimei Gao Jingyi Shi Qingfeng Sheng Lulu Zheng Junmei Zhou Xueli Wang
author_sort	Shenghua Yu
title	Hydrogen Promotes the M1 Macrophage Conversion During the Polarization of Macrophages in Necrotizing Enterocolitis
title_short	Hydrogen Promotes the M1 Macrophage Conversion During the Polarization of Macrophages in Necrotizing Enterocolitis
title_full	Hydrogen Promotes the M1 Macrophage Conversion During the Polarization of Macrophages in Necrotizing Enterocolitis
title_fullStr	Hydrogen Promotes the M1 Macrophage Conversion During the Polarization of Macrophages in Necrotizing Enterocolitis
title_full_unstemmed	Hydrogen Promotes the M1 Macrophage Conversion During the Polarization of Macrophages in Necrotizing Enterocolitis
title_sort	hydrogen promotes the m1 macrophage conversion during the polarization of macrophages in necrotizing enterocolitis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/351c408728f24e9b980f00f9dcdb02eb
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Hydrogen Promotes the M1 Macrophage Conversion During the Polarization of Macrophages in Necrotizing Enterocolitis

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