DNA structure at the plasmid origin-of-transfer indicates its potential transfer range

Abstract Horizontal gene transfer via plasmid conjugation enables antimicrobial resistance (AMR) to spread among bacteria and is a major health concern. The range of potential transfer hosts of a particular conjugative plasmid is characterised by its mobility (MOB) group, which is currently determin...

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Autores principales:	Jan Zrimec, Aleš Lapanje
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3526ac343ab744d1b4bfc3bc143535c1
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spelling	oai:doaj.org-article:3526ac343ab744d1b4bfc3bc143535c12021-12-02T15:08:14ZDNA structure at the plasmid origin-of-transfer indicates its potential transfer range10.1038/s41598-018-20157-y2045-2322https://doaj.org/article/3526ac343ab744d1b4bfc3bc143535c12018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20157-yhttps://doaj.org/toc/2045-2322Abstract Horizontal gene transfer via plasmid conjugation enables antimicrobial resistance (AMR) to spread among bacteria and is a major health concern. The range of potential transfer hosts of a particular conjugative plasmid is characterised by its mobility (MOB) group, which is currently determined based on the amino acid sequence of the plasmid-encoded relaxase. To facilitate prediction of plasmid MOB groups, we have developed a bioinformatic procedure based on analysis of the origin-of-transfer (oriT), a merely 230 bp long non-coding plasmid DNA region that is the enzymatic substrate for the relaxase. By computationally interpreting conformational and physicochemical properties of the oriT region, which facilitate relaxase-oriT recognition and initiation of nicking, MOB groups can be resolved with over 99% accuracy. We have shown that oriT structural properties are highly conserved and can be used to discriminate among MOB groups more efficiently than the oriT nucleotide sequence. The procedure for prediction of MOB groups and potential transfer range of plasmids was implemented using published data and is available at http://dnatools.eu/MOB/plasmid.html .Jan ZrimecAleš LapanjeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jan Zrimec Aleš Lapanje DNA structure at the plasmid origin-of-transfer indicates its potential transfer range
description	Abstract Horizontal gene transfer via plasmid conjugation enables antimicrobial resistance (AMR) to spread among bacteria and is a major health concern. The range of potential transfer hosts of a particular conjugative plasmid is characterised by its mobility (MOB) group, which is currently determined based on the amino acid sequence of the plasmid-encoded relaxase. To facilitate prediction of plasmid MOB groups, we have developed a bioinformatic procedure based on analysis of the origin-of-transfer (oriT), a merely 230 bp long non-coding plasmid DNA region that is the enzymatic substrate for the relaxase. By computationally interpreting conformational and physicochemical properties of the oriT region, which facilitate relaxase-oriT recognition and initiation of nicking, MOB groups can be resolved with over 99% accuracy. We have shown that oriT structural properties are highly conserved and can be used to discriminate among MOB groups more efficiently than the oriT nucleotide sequence. The procedure for prediction of MOB groups and potential transfer range of plasmids was implemented using published data and is available at http://dnatools.eu/MOB/plasmid.html .
format	article
author	Jan Zrimec Aleš Lapanje
author_facet	Jan Zrimec Aleš Lapanje
author_sort	Jan Zrimec
title	DNA structure at the plasmid origin-of-transfer indicates its potential transfer range
title_short	DNA structure at the plasmid origin-of-transfer indicates its potential transfer range
title_full	DNA structure at the plasmid origin-of-transfer indicates its potential transfer range
title_fullStr	DNA structure at the plasmid origin-of-transfer indicates its potential transfer range
title_full_unstemmed	DNA structure at the plasmid origin-of-transfer indicates its potential transfer range
title_sort	dna structure at the plasmid origin-of-transfer indicates its potential transfer range
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/3526ac343ab744d1b4bfc3bc143535c1
work_keys_str_mv	AT janzrimec dnastructureattheplasmidoriginoftransferindicatesitspotentialtransferrange AT aleslapanje dnastructureattheplasmidoriginoftransferindicatesitspotentialtransferrange
_version_	1718388178307514368

DNA structure at the plasmid origin-of-transfer indicates its potential transfer range

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