Engineering lithoheterotrophy in an obligate chemolithoautotrophic Fe(II) oxidizing bacterium

Abstract Neutrophilic Fe(II) oxidizing bacteria like Mariprofundus ferrooxydans are obligate chemolithoautotrophic bacteria that play an important role in the biogeochemical cycling of iron and other elements in multiple environments. These bacteria generally exhibit a singular metabolic mode of gro...

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Autores principales:	Abhiney Jain, Jeffrey A. Gralnick
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3526b6c8eee94343b0f83cb3f8cc145e
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spelling	oai:doaj.org-article:3526b6c8eee94343b0f83cb3f8cc145e2021-12-02T14:16:26ZEngineering lithoheterotrophy in an obligate chemolithoautotrophic Fe(II) oxidizing bacterium10.1038/s41598-021-81412-32045-2322https://doaj.org/article/3526b6c8eee94343b0f83cb3f8cc145e2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81412-3https://doaj.org/toc/2045-2322Abstract Neutrophilic Fe(II) oxidizing bacteria like Mariprofundus ferrooxydans are obligate chemolithoautotrophic bacteria that play an important role in the biogeochemical cycling of iron and other elements in multiple environments. These bacteria generally exhibit a singular metabolic mode of growth which prohibits comparative “omics” studies. Furthermore, these bacteria are considered non-amenable to classical genetic methods due to low cell densities, the inability to form colonies on solid medium, and production of copious amounts of insoluble iron oxyhydroxides as their metabolic byproduct. Consequently, the molecular and biochemical understanding of these bacteria remains speculative despite the availability of substantial genomic information. Here we develop the first genetic system in neutrophilic Fe(II) oxidizing bacterium and use it to engineer lithoheterotrophy in M. ferrooxydans, a metabolism that has been speculated but not experimentally validated. This synthetic biology approach could be extended to gain physiological understanding and domesticate other bacteria that grow using a single metabolic mode.Abhiney JainJeffrey A. GralnickNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abhiney Jain Jeffrey A. Gralnick Engineering lithoheterotrophy in an obligate chemolithoautotrophic Fe(II) oxidizing bacterium
description	Abstract Neutrophilic Fe(II) oxidizing bacteria like Mariprofundus ferrooxydans are obligate chemolithoautotrophic bacteria that play an important role in the biogeochemical cycling of iron and other elements in multiple environments. These bacteria generally exhibit a singular metabolic mode of growth which prohibits comparative “omics” studies. Furthermore, these bacteria are considered non-amenable to classical genetic methods due to low cell densities, the inability to form colonies on solid medium, and production of copious amounts of insoluble iron oxyhydroxides as their metabolic byproduct. Consequently, the molecular and biochemical understanding of these bacteria remains speculative despite the availability of substantial genomic information. Here we develop the first genetic system in neutrophilic Fe(II) oxidizing bacterium and use it to engineer lithoheterotrophy in M. ferrooxydans, a metabolism that has been speculated but not experimentally validated. This synthetic biology approach could be extended to gain physiological understanding and domesticate other bacteria that grow using a single metabolic mode.
format	article
author	Abhiney Jain Jeffrey A. Gralnick
author_facet	Abhiney Jain Jeffrey A. Gralnick
author_sort	Abhiney Jain
title	Engineering lithoheterotrophy in an obligate chemolithoautotrophic Fe(II) oxidizing bacterium
title_short	Engineering lithoheterotrophy in an obligate chemolithoautotrophic Fe(II) oxidizing bacterium
title_full	Engineering lithoheterotrophy in an obligate chemolithoautotrophic Fe(II) oxidizing bacterium
title_fullStr	Engineering lithoheterotrophy in an obligate chemolithoautotrophic Fe(II) oxidizing bacterium
title_full_unstemmed	Engineering lithoheterotrophy in an obligate chemolithoautotrophic Fe(II) oxidizing bacterium
title_sort	engineering lithoheterotrophy in an obligate chemolithoautotrophic fe(ii) oxidizing bacterium
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3526b6c8eee94343b0f83cb3f8cc145e
work_keys_str_mv	AT abhineyjain engineeringlithoheterotrophyinanobligatechemolithoautotrophicfeiioxidizingbacterium AT jeffreyagralnick engineeringlithoheterotrophyinanobligatechemolithoautotrophicfeiioxidizingbacterium
_version_	1718391658626678784

Engineering lithoheterotrophy in an obligate chemolithoautotrophic Fe(II) oxidizing bacterium

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