TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum

B cells played an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, C57BL/6 mice were percutaneously infected by S. japonicum for 5–6 weeks. The percent...

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Autores principales:	Haixia Wei, Hongyan Xie, Jiale Qu, Anqi Xie, Shihao Xie, He Huang, Jiajie Li, Chao Fang, Feihu Shi, Huaina Qiu, Yanwei Qi, Xu Tian, Quan Yang, Jun Huang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/35319f5ef25a41a3b12f606f5b40940e
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spelling	oai:doaj.org-article:35319f5ef25a41a3b12f606f5b40940e2021-11-25T06:33:06ZTLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum1935-27271935-2735https://doaj.org/article/35319f5ef25a41a3b12f606f5b40940e2021-11-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8598019/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735B cells played an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, C57BL/6 mice were percutaneously infected by S. japonicum for 5–6 weeks. The percentages and numbers of B cells increased in the infected mice (p < 0.05), and many activation and function associated molecules were also changed on B cells. More splenic cells of the infected mice expressed TLR7, and B cells were served as the main cell population. Moreover, a lower level of soluble egg antigen (SEA) specific antibody and less activation associated molecules were found on the surface of splenic B cells from S. japonicum infected TLR7 gene knockout (TLR7 KO) mice compared to infected wild type (WT) mice (p < 0.05). Additionally, SEA showed a little higher ability in inducing the activation of B cells from naive WT mice than TLR7 KO mice (p < 0.05). Finally, the effects of TLR7 on B cells are dependent on the activation of NF-κB p65. Altogether, TLR7 was found modulating the splenic B cell responses in S. japonicum infected C57BL/6 mice. Author summary Schistosomiasis seriously jeopardizes public health and social development in tropical and subtropical regions. B cells play an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, we found the percentage and numbers of B cells increased in the infected mice (p < 0.05), and the expression of many activation and function associated molecules were also changed on B cells. B cells were served as the main cell population expressed TLR7. When TLR7 gene was knockout, the soluble egg antigen (SEA) specific antibody and activation associated molecules were decreased in S. japonicum infected mice. Additionally, SEA could induce the activation of B cells by TLR7. Finally, we found the effects of TLR7 on B cells are dependent on the activation of NF-κB p65. In this study, the characteristic of B cells in the spleen of S. japonicum infected C57BL/6 mice was explored, and the role of TLR7 on the progress of B cell activation and differentiation was investigated.Haixia WeiHongyan XieJiale QuAnqi XieShihao XieHe HuangJiajie LiChao FangFeihu ShiHuaina QiuYanwei QiXu TianQuan YangJun HuangPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Haixia Wei Hongyan Xie Jiale Qu Anqi Xie Shihao Xie He Huang Jiajie Li Chao Fang Feihu Shi Huaina Qiu Yanwei Qi Xu Tian Quan Yang Jun Huang TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum
description	B cells played an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, C57BL/6 mice were percutaneously infected by S. japonicum for 5–6 weeks. The percentages and numbers of B cells increased in the infected mice (p < 0.05), and many activation and function associated molecules were also changed on B cells. More splenic cells of the infected mice expressed TLR7, and B cells were served as the main cell population. Moreover, a lower level of soluble egg antigen (SEA) specific antibody and less activation associated molecules were found on the surface of splenic B cells from S. japonicum infected TLR7 gene knockout (TLR7 KO) mice compared to infected wild type (WT) mice (p < 0.05). Additionally, SEA showed a little higher ability in inducing the activation of B cells from naive WT mice than TLR7 KO mice (p < 0.05). Finally, the effects of TLR7 on B cells are dependent on the activation of NF-κB p65. Altogether, TLR7 was found modulating the splenic B cell responses in S. japonicum infected C57BL/6 mice. Author summary Schistosomiasis seriously jeopardizes public health and social development in tropical and subtropical regions. B cells play an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, we found the percentage and numbers of B cells increased in the infected mice (p < 0.05), and the expression of many activation and function associated molecules were also changed on B cells. B cells were served as the main cell population expressed TLR7. When TLR7 gene was knockout, the soluble egg antigen (SEA) specific antibody and activation associated molecules were decreased in S. japonicum infected mice. Additionally, SEA could induce the activation of B cells by TLR7. Finally, we found the effects of TLR7 on B cells are dependent on the activation of NF-κB p65. In this study, the characteristic of B cells in the spleen of S. japonicum infected C57BL/6 mice was explored, and the role of TLR7 on the progress of B cell activation and differentiation was investigated.
format	article
author	Haixia Wei Hongyan Xie Jiale Qu Anqi Xie Shihao Xie He Huang Jiajie Li Chao Fang Feihu Shi Huaina Qiu Yanwei Qi Xu Tian Quan Yang Jun Huang
author_facet	Haixia Wei Hongyan Xie Jiale Qu Anqi Xie Shihao Xie He Huang Jiajie Li Chao Fang Feihu Shi Huaina Qiu Yanwei Qi Xu Tian Quan Yang Jun Huang
author_sort	Haixia Wei
title	TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum
title_short	TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum
title_full	TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum
title_fullStr	TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum
title_full_unstemmed	TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum
title_sort	tlr7 modulating b-cell immune responses in the spleen of c57bl/6 mice infected with schistosoma japonicum
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/35319f5ef25a41a3b12f606f5b40940e
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TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum

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