The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy

Behzad Nasseri,1– 3 Mustafa Turk,4 Kemal Kosemehmetoglu,5 Murat Kaya,2 Erhan Piskin,1 Navid Rabiee,6 Thomas J Webster7 1Chemical Engineering Department, Bioengineering Division and Bioengineering Centre, Hacettepe University, Ankara 06800, Turkey; 2Chemical Engineering and Applied Chemistr...

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Autores principales:	Nasseri B, Turk M, Kosemehmetoglu K, Kaya M, Piskin E, Rabiee N, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	plasmonic photothermal therapy gold nanoparticles colorectal cells pathology Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/35347cd9389846a0882884b65b72c942
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spelling	oai:doaj.org-article:35347cd9389846a0882884b65b72c9422021-12-02T08:54:58ZThe Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy1178-2013https://doaj.org/article/35347cd9389846a0882884b65b72c9422020-04-01T00:00:00Zhttps://www.dovepress.com/the-pimpled-gold-nanosphere-a-superior-candidate-for-plasmonic-phototh-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Behzad Nasseri,1– 3 Mustafa Turk,4 Kemal Kosemehmetoglu,5 Murat Kaya,2 Erhan Piskin,1 Navid Rabiee,6 Thomas J Webster7 1Chemical Engineering Department, Bioengineering Division and Bioengineering Centre, Hacettepe University, Ankara 06800, Turkey; 2Chemical Engineering and Applied Chemistry Department, Atilim University, Ankara 06830, Turkey; 3Bioscience Faculty, Shahid Beheshti University, Tehran, Iran; 4Bioengineering Department, Kirikkale University, Kirikkale, Turkey; 5Department of Pathology, Hacettepe Medical Science University, Ankara, Turkey; 6Department of Chemistry, Shahid Beheshti University, Tehran, Iran; 7Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USACorrespondence: Behzad Nasseri; Thomas J Webster Email bnaseri2000@gmail.com; th.webster@neu.eduBackground: The development of highly efficient nanoparticles to convert light to heat for anti-cancer applications is quite a challenging field of research.Methods: In this study, we synthesized unique pimpled gold nanospheres (PGNSs) for plasmonic photothermal therapy (PPTT). The light-to-heat conversion capability of PGNSs and PPTT damage at the cellular level were investigated using a tissue phantom model. The ability of PGNSs to induce robust cellular damage was studied during cytotoxicity tests on colorectal adenocarcinoma (DLD-1) and fibroblast cell lines. Further, a numerical model of plasmonic (COMSOL Multiphysics) properties was used with the PPTT experimental assays.Results: A low cytotoxic effect of thiolated polyethylene glycol (SH-PEG400-SH-) was observed which improved the biocompatibility of PGNSs to maintain 89.4% cell viability during cytometry assays (in terms of fibroblast cells for 24 hrs at a concentration of 300 μg/mL). The heat generated from the nanoparticle-mediated phantom models resulted in ΔT=30°C, ΔT=23.1°C and ΔT=21°C for the PGNSs, AuNRs, and AuNPs, respectively (at a 300 μg/mL concentration and for 325 sec). For the in vitro assays of PPTT on cancer cells, the PGNS group induced a 68.78% lethality (apoptosis) on DLD-1 cells. Fluorescence microscopy results showed the destruction of cell membranes and nuclei for the PPTT group. Experiments further revealed a penetration depth of sufficient PPTT damage in a physical tumor model after hematoxylin and eosin (H&E) staining through pathological studies (at depths of 2, 3 and 4 cm). Severe structural damages were observed in the tissue model through an 808-nm laser exposed to the PGNSs.Conclusion: Collectively, such results show much promise for the use of the present PGNSs and photothermal therapy for numerous anti-cancer applications.Keywords: plasmonic, photothermal therapy, gold nanoparticles, colorectal cells, pathologyNasseri BTurk MKosemehmetoglu KKaya MPiskin ERabiee NWebster TJDove Medical Pressarticleplasmonicphotothermal therapygold nanoparticlescolorectal cellspathologyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 2903-2920 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	plasmonic photothermal therapy gold nanoparticles colorectal cells pathology Medicine (General) R5-920
spellingShingle	plasmonic photothermal therapy gold nanoparticles colorectal cells pathology Medicine (General) R5-920 Nasseri B Turk M Kosemehmetoglu K Kaya M Piskin E Rabiee N Webster TJ The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy
description	Behzad Nasseri,1– 3 Mustafa Turk,4 Kemal Kosemehmetoglu,5 Murat Kaya,2 Erhan Piskin,1 Navid Rabiee,6 Thomas J Webster7 1Chemical Engineering Department, Bioengineering Division and Bioengineering Centre, Hacettepe University, Ankara 06800, Turkey; 2Chemical Engineering and Applied Chemistry Department, Atilim University, Ankara 06830, Turkey; 3Bioscience Faculty, Shahid Beheshti University, Tehran, Iran; 4Bioengineering Department, Kirikkale University, Kirikkale, Turkey; 5Department of Pathology, Hacettepe Medical Science University, Ankara, Turkey; 6Department of Chemistry, Shahid Beheshti University, Tehran, Iran; 7Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USACorrespondence: Behzad Nasseri; Thomas J Webster Email bnaseri2000@gmail.com; th.webster@neu.eduBackground: The development of highly efficient nanoparticles to convert light to heat for anti-cancer applications is quite a challenging field of research.Methods: In this study, we synthesized unique pimpled gold nanospheres (PGNSs) for plasmonic photothermal therapy (PPTT). The light-to-heat conversion capability of PGNSs and PPTT damage at the cellular level were investigated using a tissue phantom model. The ability of PGNSs to induce robust cellular damage was studied during cytotoxicity tests on colorectal adenocarcinoma (DLD-1) and fibroblast cell lines. Further, a numerical model of plasmonic (COMSOL Multiphysics) properties was used with the PPTT experimental assays.Results: A low cytotoxic effect of thiolated polyethylene glycol (SH-PEG400-SH-) was observed which improved the biocompatibility of PGNSs to maintain 89.4% cell viability during cytometry assays (in terms of fibroblast cells for 24 hrs at a concentration of 300 μg/mL). The heat generated from the nanoparticle-mediated phantom models resulted in ΔT=30°C, ΔT=23.1°C and ΔT=21°C for the PGNSs, AuNRs, and AuNPs, respectively (at a 300 μg/mL concentration and for 325 sec). For the in vitro assays of PPTT on cancer cells, the PGNS group induced a 68.78% lethality (apoptosis) on DLD-1 cells. Fluorescence microscopy results showed the destruction of cell membranes and nuclei for the PPTT group. Experiments further revealed a penetration depth of sufficient PPTT damage in a physical tumor model after hematoxylin and eosin (H&E) staining through pathological studies (at depths of 2, 3 and 4 cm). Severe structural damages were observed in the tissue model through an 808-nm laser exposed to the PGNSs.Conclusion: Collectively, such results show much promise for the use of the present PGNSs and photothermal therapy for numerous anti-cancer applications.Keywords: plasmonic, photothermal therapy, gold nanoparticles, colorectal cells, pathology
format	article
author	Nasseri B Turk M Kosemehmetoglu K Kaya M Piskin E Rabiee N Webster TJ
author_facet	Nasseri B Turk M Kosemehmetoglu K Kaya M Piskin E Rabiee N Webster TJ
author_sort	Nasseri B
title	The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy
title_short	The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy
title_full	The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy
title_fullStr	The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy
title_full_unstemmed	The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy
title_sort	pimpled gold nanosphere: a superior candidate for plasmonic photothermal therapy
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/35347cd9389846a0882884b65b72c942
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The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy

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