BRAHMA ATPase of the SWI/SNF chromatin remodeling complex acts as a positive regulator of gibberellin-mediated responses in arabidopsis.

SWI/SNF chromatin remodeling complexes perform a pivotal function in the regulation of eukaryotic gene expression. Arabidopsis (Arabidopsis thaliana) mutants in major SWI/SNF subunits display embryo-lethal or dwarf phenotypes, indicating their critical role in molecular pathways controlling developm...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Rafal Archacki, Daniel Buszewicz, Tomasz J Sarnowski, Elzbieta Sarnowska, Anna T Rolicka, Takayuki Tohge, Alisdair R Fernie, Yusuke Jikumaru, Maciej Kotlinski, Roksana Iwanicka-Nowicka, Katarzyna Kalisiak, Jacek Patryn, Joanna Halibart-Puzio, Yuji Kamiya, Seth J Davis, Marta K Koblowska, Andrzej Jerzmanowski
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2013
Materias:
R
Q
Acceso en línea:https://doaj.org/article/3535582c1a4e4affa366cf6f0c56f159
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:3535582c1a4e4affa366cf6f0c56f159
record_format dspace
spelling oai:doaj.org-article:3535582c1a4e4affa366cf6f0c56f1592021-11-18T07:53:58ZBRAHMA ATPase of the SWI/SNF chromatin remodeling complex acts as a positive regulator of gibberellin-mediated responses in arabidopsis.1932-620310.1371/journal.pone.0058588https://doaj.org/article/3535582c1a4e4affa366cf6f0c56f1592013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23536800/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203SWI/SNF chromatin remodeling complexes perform a pivotal function in the regulation of eukaryotic gene expression. Arabidopsis (Arabidopsis thaliana) mutants in major SWI/SNF subunits display embryo-lethal or dwarf phenotypes, indicating their critical role in molecular pathways controlling development and growth. As gibberellins (GA) are major positive regulators of plant growth, we wanted to establish whether there is a link between SWI/SNF and GA signaling in Arabidopsis. This study revealed that in brm-1 plants, depleted in SWI/SNF BRAHMA (BRM) ATPase, a number of GA-related phenotypic traits are GA-sensitive and that the loss of BRM results in markedly decreased level of endogenous bioactive GA. Transcriptional profiling of brm-1 and the GA biosynthesis mutant ga1-3, as well as the ga1-3/brm-1 double mutant demonstrated that BRM affects the expression of a large set of GA-responsive genes including genes responsible for GA biosynthesis and signaling. Furthermore, we found that BRM acts as an activator and directly associates with promoters of GA3ox1, a GA biosynthetic gene, and SCL3, implicated in positive regulation of the GA pathway. Many GA-responsive gene expression alterations in the brm-1 mutant are likely due to depleted levels of active GAs. However, the analysis of genetic interactions between BRM and the DELLA GA pathway repressors, revealed that BRM also acts on GA-responsive genes independently of its effect on GA level. Given the central position occupied by SWI/SNF complexes within regulatory networks controlling fundamental biological processes, the identification of diverse functional intersections of BRM with GA-dependent processes in this study suggests a role for SWI/SNF in facilitating crosstalk between GA-mediated regulation and other cellular pathways.Rafal ArchackiDaniel BuszewiczTomasz J SarnowskiElzbieta SarnowskaAnna T RolickaTakayuki TohgeAlisdair R FernieYusuke JikumaruMaciej KotlinskiRoksana Iwanicka-NowickaKatarzyna KalisiakJacek PatrynJoanna Halibart-PuzioYuji KamiyaSeth J DavisMarta K KoblowskaAndrzej JerzmanowskiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58588 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Rafal Archacki
Daniel Buszewicz
Tomasz J Sarnowski
Elzbieta Sarnowska
Anna T Rolicka
Takayuki Tohge
Alisdair R Fernie
Yusuke Jikumaru
Maciej Kotlinski
Roksana Iwanicka-Nowicka
Katarzyna Kalisiak
Jacek Patryn
Joanna Halibart-Puzio
Yuji Kamiya
Seth J Davis
Marta K Koblowska
Andrzej Jerzmanowski
BRAHMA ATPase of the SWI/SNF chromatin remodeling complex acts as a positive regulator of gibberellin-mediated responses in arabidopsis.
description SWI/SNF chromatin remodeling complexes perform a pivotal function in the regulation of eukaryotic gene expression. Arabidopsis (Arabidopsis thaliana) mutants in major SWI/SNF subunits display embryo-lethal or dwarf phenotypes, indicating their critical role in molecular pathways controlling development and growth. As gibberellins (GA) are major positive regulators of plant growth, we wanted to establish whether there is a link between SWI/SNF and GA signaling in Arabidopsis. This study revealed that in brm-1 plants, depleted in SWI/SNF BRAHMA (BRM) ATPase, a number of GA-related phenotypic traits are GA-sensitive and that the loss of BRM results in markedly decreased level of endogenous bioactive GA. Transcriptional profiling of brm-1 and the GA biosynthesis mutant ga1-3, as well as the ga1-3/brm-1 double mutant demonstrated that BRM affects the expression of a large set of GA-responsive genes including genes responsible for GA biosynthesis and signaling. Furthermore, we found that BRM acts as an activator and directly associates with promoters of GA3ox1, a GA biosynthetic gene, and SCL3, implicated in positive regulation of the GA pathway. Many GA-responsive gene expression alterations in the brm-1 mutant are likely due to depleted levels of active GAs. However, the analysis of genetic interactions between BRM and the DELLA GA pathway repressors, revealed that BRM also acts on GA-responsive genes independently of its effect on GA level. Given the central position occupied by SWI/SNF complexes within regulatory networks controlling fundamental biological processes, the identification of diverse functional intersections of BRM with GA-dependent processes in this study suggests a role for SWI/SNF in facilitating crosstalk between GA-mediated regulation and other cellular pathways.
format article
author Rafal Archacki
Daniel Buszewicz
Tomasz J Sarnowski
Elzbieta Sarnowska
Anna T Rolicka
Takayuki Tohge
Alisdair R Fernie
Yusuke Jikumaru
Maciej Kotlinski
Roksana Iwanicka-Nowicka
Katarzyna Kalisiak
Jacek Patryn
Joanna Halibart-Puzio
Yuji Kamiya
Seth J Davis
Marta K Koblowska
Andrzej Jerzmanowski
author_facet Rafal Archacki
Daniel Buszewicz
Tomasz J Sarnowski
Elzbieta Sarnowska
Anna T Rolicka
Takayuki Tohge
Alisdair R Fernie
Yusuke Jikumaru
Maciej Kotlinski
Roksana Iwanicka-Nowicka
Katarzyna Kalisiak
Jacek Patryn
Joanna Halibart-Puzio
Yuji Kamiya
Seth J Davis
Marta K Koblowska
Andrzej Jerzmanowski
author_sort Rafal Archacki
title BRAHMA ATPase of the SWI/SNF chromatin remodeling complex acts as a positive regulator of gibberellin-mediated responses in arabidopsis.
title_short BRAHMA ATPase of the SWI/SNF chromatin remodeling complex acts as a positive regulator of gibberellin-mediated responses in arabidopsis.
title_full BRAHMA ATPase of the SWI/SNF chromatin remodeling complex acts as a positive regulator of gibberellin-mediated responses in arabidopsis.
title_fullStr BRAHMA ATPase of the SWI/SNF chromatin remodeling complex acts as a positive regulator of gibberellin-mediated responses in arabidopsis.
title_full_unstemmed BRAHMA ATPase of the SWI/SNF chromatin remodeling complex acts as a positive regulator of gibberellin-mediated responses in arabidopsis.
title_sort brahma atpase of the swi/snf chromatin remodeling complex acts as a positive regulator of gibberellin-mediated responses in arabidopsis.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/3535582c1a4e4affa366cf6f0c56f159
work_keys_str_mv AT rafalarchacki brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT danielbuszewicz brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT tomaszjsarnowski brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT elzbietasarnowska brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT annatrolicka brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT takayukitohge brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT alisdairrfernie brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT yusukejikumaru brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT maciejkotlinski brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT roksanaiwanickanowicka brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT katarzynakalisiak brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT jacekpatryn brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT joannahalibartpuzio brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT yujikamiya brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT sethjdavis brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT martakkoblowska brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
AT andrzejjerzmanowski brahmaatpaseoftheswisnfchromatinremodelingcomplexactsasapositiveregulatorofgibberellinmediatedresponsesinarabidopsis
_version_ 1718422777280593920