Tracing Asian seabass individuals to single fish farms using microsatellites.

Traceability through physical labels is well established, but it is not highly reliable as physical labels can be easily changed or lost. Application of DNA markers to the traceability of food plays an increasingly important role for consumer protection and confidence building. In this study, we tes...

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Autores principales:	Gen Hua Yue, Jun Hong Xia, Peng Liu, Feng Liu, Fei Sun, Grace Lin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3541c8894f6a47808484670b3f174601
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spelling	oai:doaj.org-article:3541c8894f6a47808484670b3f1746012021-11-18T08:04:02ZTracing Asian seabass individuals to single fish farms using microsatellites.1932-620310.1371/journal.pone.0052721https://doaj.org/article/3541c8894f6a47808484670b3f1746012012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23285169/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Traceability through physical labels is well established, but it is not highly reliable as physical labels can be easily changed or lost. Application of DNA markers to the traceability of food plays an increasingly important role for consumer protection and confidence building. In this study, we tested the efficiency of 16 polymorphic microsatellites and their combinations for tracing 368 fish to four populations where they originated. Using the maximum likelihood and Bayesian methods, three most efficient microsatellites were required to assign over 95% of fish to the correct populations. Selection of markers based on the assignment score estimated with the software WHICHLOCI was most effective in choosing markers for individual assignment, followed by the selection based on the allele number of individual markers. By combining rapid DNA extraction, and high-throughput genotyping of selected microsatellites, it is possible to conduct routine genetic traceability with high accuracy in Asian seabass.Gen Hua YueJun Hong XiaPeng LiuFeng LiuFei SunGrace LinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52721 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gen Hua Yue Jun Hong Xia Peng Liu Feng Liu Fei Sun Grace Lin Tracing Asian seabass individuals to single fish farms using microsatellites.
description	Traceability through physical labels is well established, but it is not highly reliable as physical labels can be easily changed or lost. Application of DNA markers to the traceability of food plays an increasingly important role for consumer protection and confidence building. In this study, we tested the efficiency of 16 polymorphic microsatellites and their combinations for tracing 368 fish to four populations where they originated. Using the maximum likelihood and Bayesian methods, three most efficient microsatellites were required to assign over 95% of fish to the correct populations. Selection of markers based on the assignment score estimated with the software WHICHLOCI was most effective in choosing markers for individual assignment, followed by the selection based on the allele number of individual markers. By combining rapid DNA extraction, and high-throughput genotyping of selected microsatellites, it is possible to conduct routine genetic traceability with high accuracy in Asian seabass.
format	article
author	Gen Hua Yue Jun Hong Xia Peng Liu Feng Liu Fei Sun Grace Lin
author_facet	Gen Hua Yue Jun Hong Xia Peng Liu Feng Liu Fei Sun Grace Lin
author_sort	Gen Hua Yue
title	Tracing Asian seabass individuals to single fish farms using microsatellites.
title_short	Tracing Asian seabass individuals to single fish farms using microsatellites.
title_full	Tracing Asian seabass individuals to single fish farms using microsatellites.
title_fullStr	Tracing Asian seabass individuals to single fish farms using microsatellites.
title_full_unstemmed	Tracing Asian seabass individuals to single fish farms using microsatellites.
title_sort	tracing asian seabass individuals to single fish farms using microsatellites.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/3541c8894f6a47808484670b3f174601
work_keys_str_mv	AT genhuayue tracingasianseabassindividualstosinglefishfarmsusingmicrosatellites AT junhongxia tracingasianseabassindividualstosinglefishfarmsusingmicrosatellites AT pengliu tracingasianseabassindividualstosinglefishfarmsusingmicrosatellites AT fengliu tracingasianseabassindividualstosinglefishfarmsusingmicrosatellites AT feisun tracingasianseabassindividualstosinglefishfarmsusingmicrosatellites AT gracelin tracingasianseabassindividualstosinglefishfarmsusingmicrosatellites
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Tracing Asian seabass individuals to single fish farms using microsatellites.

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