SnO2-Doped ZnO/Reduced Graphene Oxide Nanocomposites: Synthesis, Characterization, and Improved Anticancer Activity via Oxidative Stress Pathway

Maqusood Ahamed,1 Mohd Javed Akhtar,1 MA Majeed Khan,1 Hisham A Alhadlaq1,2 1King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia; 2Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaCorrespondence: Maqusoo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ahamed M, Akhtar MJ, Khan MAM, Alhadlaq HA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	zno nanoparticles nanocomposites improved anticancer activity better selectivity reactive oxygen species caspase-3 breast cancer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3545232a3d1c47f3bfe528f4c8205e89
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3545232a3d1c47f3bfe528f4c8205e89
record_format	dspace
spelling	oai:doaj.org-article:3545232a3d1c47f3bfe528f4c8205e892021-12-02T14:24:01ZSnO2-Doped ZnO/Reduced Graphene Oxide Nanocomposites: Synthesis, Characterization, and Improved Anticancer Activity via Oxidative Stress Pathway1178-2013https://doaj.org/article/3545232a3d1c47f3bfe528f4c8205e892021-01-01T00:00:00Zhttps://www.dovepress.com/sno2-doped-znoreduced-graphene-oxide-nanocomposites-synthesis-characte-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Maqusood Ahamed,1 Mohd Javed Akhtar,1 MA Majeed Khan,1 Hisham A Alhadlaq1,2 1King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia; 2Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaCorrespondence: Maqusood AhamedKing Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi ArabiaEmail mahamed@ksu.edu.saBackground: Therapeutic selectivity and drug resistance are critical issues in cancer therapy. Currently, zinc oxide nanoparticles (ZnO NPs) hold considerable promise to tackle this problem due to their tunable physicochemical properties. This work was designed to prepare SnO2-doped ZnO NPs/reduced graphene oxide nanocomposites (SnO2-ZnO/rGO NCs) with enhanced anticancer activity and better biocompatibility than those of pure ZnO NPs.Materials and Methods: Pure ZnO NPs, SnO2-doped ZnO (SnO2-ZnO) NPs, and SnO2-ZnO/rGO NCs were prepared via a facile hydrothermal method. Prepared samples were characterized by field emission transmission electron microscopy (FETEM), energy dispersive spectroscopy (EDS), field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), ultraviolet-visible (UV-VIS) spectrometer, and dynamic light scattering (DLS) techniques. Selectivity and anticancer activity of prepared samples were assessed in human breast cancer (MCF-7) and human normal breast epithelial (MCF10A) cells. Possible mechanisms of anticancer activity of prepared samples were explored through oxidative stress pathway.Results: XRD spectra of SnO2-ZnO/rGO NCs confirmed the formation of single-phase of hexagonal wurtzite ZnO. High resolution TEM and SEM mapping showed homogenous distribution of SnO2 and rGO in ZnO NPs with high quality lattice fringes without any distortion. Band gap energy of SnO2-ZnO/rGO NCs was lower compared to SnO2-ZnO NPs and pure ZnO NPs. The SnO2-ZnO/rGO NCs exhibited significantly higher anticancer activity against MCF-7 cancer cells than those of SnO2-ZnO NPs and ZnO NPs. The SnO2-ZnO/rGO NCs induced apoptotic response through the upregulation of caspase-3 gene and depletion of mitochondrial membrane potential. Mechanistic study indicated that SnO2-ZnO/rGO NCs kill cancer cells through oxidative stress pathway. Moreover, biocompatibility of SnO2-ZnO/rGO NCs was also higher against normal breast epithelial (MCF10A cells) in comparison to SnO2-ZnO NPs and ZnO NPs.Conclusion: SnO2-ZnO/rGO NCs showed enhanced anticancer activity and better biocompatibility than SnO2-ZnO NPs and pure ZnO NPs. This work suggested a new approach to improve the selectivity and anticancer activity of ZnO NPs. Studies on antitumor activity of SnO2-ZnO/rGO NCs in animal models are further warranted.Keywords: ZnO nanocomposites, improved anticancer activity, better selectivity, reactive oxygen species, caspase-3, breast cancerAhamed MAkhtar MJKhan MAMAlhadlaq HADove Medical Pressarticlezno nanoparticlesnanocompositesimproved anticancer activitybetter selectivityreactive oxygen speciescaspase-3breast cancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 89-104 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	zno nanoparticles nanocomposites improved anticancer activity better selectivity reactive oxygen species caspase-3 breast cancer Medicine (General) R5-920
spellingShingle	zno nanoparticles nanocomposites improved anticancer activity better selectivity reactive oxygen species caspase-3 breast cancer Medicine (General) R5-920 Ahamed M Akhtar MJ Khan MAM Alhadlaq HA SnO2-Doped ZnO/Reduced Graphene Oxide Nanocomposites: Synthesis, Characterization, and Improved Anticancer Activity via Oxidative Stress Pathway
description	Maqusood Ahamed,1 Mohd Javed Akhtar,1 MA Majeed Khan,1 Hisham A Alhadlaq1,2 1King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia; 2Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaCorrespondence: Maqusood AhamedKing Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi ArabiaEmail mahamed@ksu.edu.saBackground: Therapeutic selectivity and drug resistance are critical issues in cancer therapy. Currently, zinc oxide nanoparticles (ZnO NPs) hold considerable promise to tackle this problem due to their tunable physicochemical properties. This work was designed to prepare SnO2-doped ZnO NPs/reduced graphene oxide nanocomposites (SnO2-ZnO/rGO NCs) with enhanced anticancer activity and better biocompatibility than those of pure ZnO NPs.Materials and Methods: Pure ZnO NPs, SnO2-doped ZnO (SnO2-ZnO) NPs, and SnO2-ZnO/rGO NCs were prepared via a facile hydrothermal method. Prepared samples were characterized by field emission transmission electron microscopy (FETEM), energy dispersive spectroscopy (EDS), field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), ultraviolet-visible (UV-VIS) spectrometer, and dynamic light scattering (DLS) techniques. Selectivity and anticancer activity of prepared samples were assessed in human breast cancer (MCF-7) and human normal breast epithelial (MCF10A) cells. Possible mechanisms of anticancer activity of prepared samples were explored through oxidative stress pathway.Results: XRD spectra of SnO2-ZnO/rGO NCs confirmed the formation of single-phase of hexagonal wurtzite ZnO. High resolution TEM and SEM mapping showed homogenous distribution of SnO2 and rGO in ZnO NPs with high quality lattice fringes without any distortion. Band gap energy of SnO2-ZnO/rGO NCs was lower compared to SnO2-ZnO NPs and pure ZnO NPs. The SnO2-ZnO/rGO NCs exhibited significantly higher anticancer activity against MCF-7 cancer cells than those of SnO2-ZnO NPs and ZnO NPs. The SnO2-ZnO/rGO NCs induced apoptotic response through the upregulation of caspase-3 gene and depletion of mitochondrial membrane potential. Mechanistic study indicated that SnO2-ZnO/rGO NCs kill cancer cells through oxidative stress pathway. Moreover, biocompatibility of SnO2-ZnO/rGO NCs was also higher against normal breast epithelial (MCF10A cells) in comparison to SnO2-ZnO NPs and ZnO NPs.Conclusion: SnO2-ZnO/rGO NCs showed enhanced anticancer activity and better biocompatibility than SnO2-ZnO NPs and pure ZnO NPs. This work suggested a new approach to improve the selectivity and anticancer activity of ZnO NPs. Studies on antitumor activity of SnO2-ZnO/rGO NCs in animal models are further warranted.Keywords: ZnO nanocomposites, improved anticancer activity, better selectivity, reactive oxygen species, caspase-3, breast cancer
format	article
author	Ahamed M Akhtar MJ Khan MAM Alhadlaq HA
author_facet	Ahamed M Akhtar MJ Khan MAM Alhadlaq HA
author_sort	Ahamed M
title	SnO2-Doped ZnO/Reduced Graphene Oxide Nanocomposites: Synthesis, Characterization, and Improved Anticancer Activity via Oxidative Stress Pathway
title_short	SnO2-Doped ZnO/Reduced Graphene Oxide Nanocomposites: Synthesis, Characterization, and Improved Anticancer Activity via Oxidative Stress Pathway
title_full	SnO2-Doped ZnO/Reduced Graphene Oxide Nanocomposites: Synthesis, Characterization, and Improved Anticancer Activity via Oxidative Stress Pathway
title_fullStr	SnO2-Doped ZnO/Reduced Graphene Oxide Nanocomposites: Synthesis, Characterization, and Improved Anticancer Activity via Oxidative Stress Pathway
title_full_unstemmed	SnO2-Doped ZnO/Reduced Graphene Oxide Nanocomposites: Synthesis, Characterization, and Improved Anticancer Activity via Oxidative Stress Pathway
title_sort	sno2-doped zno/reduced graphene oxide nanocomposites: synthesis, characterization, and improved anticancer activity via oxidative stress pathway
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/3545232a3d1c47f3bfe528f4c8205e89
work_keys_str_mv	AT ahamedm sno2dopedznoreducedgrapheneoxidenanocompositessynthesischaracterizationandimprovedanticanceractivityviaoxidativestresspathway AT akhtarmj sno2dopedznoreducedgrapheneoxidenanocompositessynthesischaracterizationandimprovedanticanceractivityviaoxidativestresspathway AT khanmam sno2dopedznoreducedgrapheneoxidenanocompositessynthesischaracterizationandimprovedanticanceractivityviaoxidativestresspathway AT alhadlaqha sno2dopedznoreducedgrapheneoxidenanocompositessynthesischaracterizationandimprovedanticanceractivityviaoxidativestresspathway
_version_	1718391419800911872

SnO2-Doped ZnO/Reduced Graphene Oxide Nanocomposites: Synthesis, Characterization, and Improved Anticancer Activity via Oxidative Stress Pathway

Ejemplares similares