Effect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice

Probiotics plays an important role in regulating gut microbiota and maintaining intestinal homeostasis. Extracellular vesicles (EVs) derived from probiotics have emerged as potential mediators of host immune response and anti-inflammatory effect. However, the anti-inflammatory effect and mechanism o...

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Autores principales:	Haining Hao, Xinyi Zhang, Lingjun Tong, Qiqi Liu, Xi Liang, Yushan Bu, Pimin Gong, Tongjie Liu, Lanwei Zhang, Yongjun Xia, Lianzhong Ai, Huaxi Yi
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Lactobacillus plantarum Q7 extracellular vesicles gut microbiota inflammatory cytokines ulcerative colitis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/354978598f32434589719061dae518e1
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spelling	oai:doaj.org-article:354978598f32434589719061dae518e12021-12-02T10:16:41ZEffect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice1664-322410.3389/fimmu.2021.777147https://doaj.org/article/354978598f32434589719061dae518e12021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.777147/fullhttps://doaj.org/toc/1664-3224Probiotics plays an important role in regulating gut microbiota and maintaining intestinal homeostasis. Extracellular vesicles (EVs) derived from probiotics have emerged as potential mediators of host immune response and anti-inflammatory effect. However, the anti-inflammatory effect and mechanism of probiotics derived EVs on inflammatory bowel disease (IBD) remains unclear. In this study, the effect of Lactobacillus plantarum Q7-derived extracellular vesicles (Q7-EVs) on gut microbiota and intestinal inflammation was investigated in C57BL/6J mice. The results showed that Q7-EVs alleviated DSS-induced colitis symptoms, including colon shortening, bleeding, and body weight loss. Consumption of Q7-EVs reduced the degree of histological damage. DSS-upregulated proinflammatory cytokine levels including IL-6, IL-1β, IL-2 and TNF-α were reduced significantly by Q7-EVs (p < 0.05). 16S rRNA sequencing results showed that Q7-EVs improved the dysregulation of gut microbiota and promoted the diversity of gut microbiota. It was observed that the pro-inflammatory bacteria (Proteobacteria) were reduced and the anti-inflammatory bacteria (Bifidobacteria and Muribaculaceae) were increased. These findings indicated that Q7-EVs might alleviate DSS-induced ulcerative colitis by regulating the gut microbiota.Haining HaoXinyi ZhangLingjun TongQiqi LiuXi LiangYushan BuPimin GongTongjie LiuLanwei ZhangYongjun XiaLianzhong AiHuaxi YiFrontiers Media S.A.articleLactobacillus plantarum Q7extracellular vesiclesgut microbiotainflammatory cytokinesulcerative colitisImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lactobacillus plantarum Q7 extracellular vesicles gut microbiota inflammatory cytokines ulcerative colitis Immunologic diseases. Allergy RC581-607
spellingShingle	Lactobacillus plantarum Q7 extracellular vesicles gut microbiota inflammatory cytokines ulcerative colitis Immunologic diseases. Allergy RC581-607 Haining Hao Xinyi Zhang Lingjun Tong Qiqi Liu Xi Liang Yushan Bu Pimin Gong Tongjie Liu Lanwei Zhang Yongjun Xia Lianzhong Ai Huaxi Yi Effect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice
description	Probiotics plays an important role in regulating gut microbiota and maintaining intestinal homeostasis. Extracellular vesicles (EVs) derived from probiotics have emerged as potential mediators of host immune response and anti-inflammatory effect. However, the anti-inflammatory effect and mechanism of probiotics derived EVs on inflammatory bowel disease (IBD) remains unclear. In this study, the effect of Lactobacillus plantarum Q7-derived extracellular vesicles (Q7-EVs) on gut microbiota and intestinal inflammation was investigated in C57BL/6J mice. The results showed that Q7-EVs alleviated DSS-induced colitis symptoms, including colon shortening, bleeding, and body weight loss. Consumption of Q7-EVs reduced the degree of histological damage. DSS-upregulated proinflammatory cytokine levels including IL-6, IL-1β, IL-2 and TNF-α were reduced significantly by Q7-EVs (p < 0.05). 16S rRNA sequencing results showed that Q7-EVs improved the dysregulation of gut microbiota and promoted the diversity of gut microbiota. It was observed that the pro-inflammatory bacteria (Proteobacteria) were reduced and the anti-inflammatory bacteria (Bifidobacteria and Muribaculaceae) were increased. These findings indicated that Q7-EVs might alleviate DSS-induced ulcerative colitis by regulating the gut microbiota.
format	article
author	Haining Hao Xinyi Zhang Lingjun Tong Qiqi Liu Xi Liang Yushan Bu Pimin Gong Tongjie Liu Lanwei Zhang Yongjun Xia Lianzhong Ai Huaxi Yi
author_facet	Haining Hao Xinyi Zhang Lingjun Tong Qiqi Liu Xi Liang Yushan Bu Pimin Gong Tongjie Liu Lanwei Zhang Yongjun Xia Lianzhong Ai Huaxi Yi
author_sort	Haining Hao
title	Effect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice
title_short	Effect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice
title_full	Effect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice
title_fullStr	Effect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice
title_full_unstemmed	Effect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice
title_sort	effect of extracellular vesicles derived from lactobacillus plantarum q7 on gut microbiota and ulcerative colitis in mice
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/354978598f32434589719061dae518e1
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Effect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice

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