Fluid-Screen as a real time dielectrophoretic method for universal microbial capture

Abstract Bacterial culture methods, e.g. Plate Counting Method (PCM), are a gold standard in the assessment of microbial contamination in multitude of human industries. They are however slow, labor intensive, and prone to manual errors. Dielectrophoresis (DEP) has shown great promise for particle se...

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Autores principales:	Robert Emanuel Weber, Janusz Jurand Petkowski, Brandye Michaels, Kamil Wisniewski, Anna Piela, Slawomir Antoszczyk, Monika Urszula Weber
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/354a82a4cb834bcdb29e1ab9c2ef6619
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spelling	oai:doaj.org-article:354a82a4cb834bcdb29e1ab9c2ef66192021-11-21T12:22:53ZFluid-Screen as a real time dielectrophoretic method for universal microbial capture10.1038/s41598-021-01600-z2045-2322https://doaj.org/article/354a82a4cb834bcdb29e1ab9c2ef66192021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01600-zhttps://doaj.org/toc/2045-2322Abstract Bacterial culture methods, e.g. Plate Counting Method (PCM), are a gold standard in the assessment of microbial contamination in multitude of human industries. They are however slow, labor intensive, and prone to manual errors. Dielectrophoresis (DEP) has shown great promise for particle separation for decades; however, it has not yet been widely applied in routine laboratory setting. This paper provides an overview of a new DEP microbial capture and separation method called Fluid-Screen (FS), that achieves very fast, efficient, reliable and repeatable capture and separation of microbial cells. Method verification experiments demonstrated that the FS system captured 100% of bacteria in test samples, a capture efficiency much higher than previously reported for similar technology. Data generated supports the superiority of the FS method as compared to the established Plate Counting Method (PCM), that is routinely used to detect bacterial contamination in healthcare, pharmacological and food industries. We demonstrate that the FS method is universal and can capture and separate different species of bacteria and fungi to viruses, from various sample matrices (i.e. human red blood cells, mammalian cells).Robert Emanuel WeberJanusz Jurand PetkowskiBrandye MichaelsKamil WisniewskiAnna PielaSlawomir AntoszczykMonika Urszula WeberNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Robert Emanuel Weber Janusz Jurand Petkowski Brandye Michaels Kamil Wisniewski Anna Piela Slawomir Antoszczyk Monika Urszula Weber Fluid-Screen as a real time dielectrophoretic method for universal microbial capture
description	Abstract Bacterial culture methods, e.g. Plate Counting Method (PCM), are a gold standard in the assessment of microbial contamination in multitude of human industries. They are however slow, labor intensive, and prone to manual errors. Dielectrophoresis (DEP) has shown great promise for particle separation for decades; however, it has not yet been widely applied in routine laboratory setting. This paper provides an overview of a new DEP microbial capture and separation method called Fluid-Screen (FS), that achieves very fast, efficient, reliable and repeatable capture and separation of microbial cells. Method verification experiments demonstrated that the FS system captured 100% of bacteria in test samples, a capture efficiency much higher than previously reported for similar technology. Data generated supports the superiority of the FS method as compared to the established Plate Counting Method (PCM), that is routinely used to detect bacterial contamination in healthcare, pharmacological and food industries. We demonstrate that the FS method is universal and can capture and separate different species of bacteria and fungi to viruses, from various sample matrices (i.e. human red blood cells, mammalian cells).
format	article
author	Robert Emanuel Weber Janusz Jurand Petkowski Brandye Michaels Kamil Wisniewski Anna Piela Slawomir Antoszczyk Monika Urszula Weber
author_facet	Robert Emanuel Weber Janusz Jurand Petkowski Brandye Michaels Kamil Wisniewski Anna Piela Slawomir Antoszczyk Monika Urszula Weber
author_sort	Robert Emanuel Weber
title	Fluid-Screen as a real time dielectrophoretic method for universal microbial capture
title_short	Fluid-Screen as a real time dielectrophoretic method for universal microbial capture
title_full	Fluid-Screen as a real time dielectrophoretic method for universal microbial capture
title_fullStr	Fluid-Screen as a real time dielectrophoretic method for universal microbial capture
title_full_unstemmed	Fluid-Screen as a real time dielectrophoretic method for universal microbial capture
title_sort	fluid-screen as a real time dielectrophoretic method for universal microbial capture
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/354a82a4cb834bcdb29e1ab9c2ef6619
work_keys_str_mv	AT robertemanuelweber fluidscreenasarealtimedielectrophoreticmethodforuniversalmicrobialcapture AT januszjurandpetkowski fluidscreenasarealtimedielectrophoreticmethodforuniversalmicrobialcapture AT brandyemichaels fluidscreenasarealtimedielectrophoreticmethodforuniversalmicrobialcapture AT kamilwisniewski fluidscreenasarealtimedielectrophoreticmethodforuniversalmicrobialcapture AT annapiela fluidscreenasarealtimedielectrophoreticmethodforuniversalmicrobialcapture AT slawomirantoszczyk fluidscreenasarealtimedielectrophoreticmethodforuniversalmicrobialcapture AT monikaurszulaweber fluidscreenasarealtimedielectrophoreticmethodforuniversalmicrobialcapture
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Fluid-Screen as a real time dielectrophoretic method for universal microbial capture

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