Function of mesenchymal stem cells following loading of gold nanotracers

Laura M Ricles1, Seung Yun Nam1,2, Konstantin Sokolov3,1, Stanislav Y Emelianov1,3, Laura J Suggs11Department of Biomedical Engineering, 2Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, TX, USA; 3Department of Imaging Physics, The University of Texas MD...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	et al, Konstantin Sokolov, Laura M Ricles, Seung Yun Nam
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/35542a2816d54e2f8a9c6793c20bcb8b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:35542a2816d54e2f8a9c6793c20bcb8b
record_format	dspace
spelling	oai:doaj.org-article:35542a2816d54e2f8a9c6793c20bcb8b2021-12-02T05:12:25ZFunction of mesenchymal stem cells following loading of gold nanotracers1176-91141178-2013https://doaj.org/article/35542a2816d54e2f8a9c6793c20bcb8b2011-02-01T00:00:00Zhttp://www.dovepress.com/function-of-mesenchymal-stem-cells-following-loading-of-gold-nanotrace-a6395https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Laura M Ricles1, Seung Yun Nam1,2, Konstantin Sokolov3,1, Stanislav Y Emelianov1,3, Laura J Suggs11Department of Biomedical Engineering, 2Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, TX, USA; 3Department of Imaging Physics, The University of Texas MD Anderson Cancer Center, Houston, TX, USABackground: Stem cells can differentiate into multiple cell types, and therefore can be used for cellular therapies, including tissue repair. However, the participation of stem cells in tissue repair and neovascularization is not well understood. Therefore, implementing a noninvasive, long-term imaging technique to track stem cells in vivo is needed to obtain a better understanding of the wound healing response. Generally, we are interested in developing an imaging approach to track mesenchymal stem cells (MSCs) in vivo after delivery via a polyethylene glycol modified fibrin matrix (PEGylated fibrin matrix) using MSCs loaded with gold nanoparticles as nanotracers. The objective of the current study was to assess the effects of loading MSCs with gold nanoparticles on cellular function.Methods: In this study, we utilized various gold nanoparticle formulations by varying size and surface coatings and assessed the efficiency of cell labeling using darkfield microscopy. We hypothesized that loading cells with gold nanotracers would not significantly alter cell function due to the inert and biocompatible characteristics of gold. The effect of nanoparticle loading on cell viability and cytotoxicity was analyzed using a LIVE/DEAD stain and an MTT assay. The ability of MSCs to differentiate into adipocytes and osteocytes after nanoparticle loading was also examined. In addition, nanoparticle loading and retention over time was assessed using inductively coupled plasma mass spectrometry (ICP-MS).Conclusion: Our results demonstrate that loading MSCs with gold nanotracers does not alter cell function and, based on the ICP-MS results, long-term imaging and tracking of MSCs is feasible. These findings strengthen the possibility of imaging MSCs in vivo, such as with optical or photoacoustic imaging, to understand better the participation and role of MSCs in neovascularization.Keywords: neovascularization, gold nanoparticles, cell tracking, stem cells, fibrin gel, optical imaging&nbsp; et alKonstantin SokolovLaura M RiclesSeung Yun NamDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 407-416 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 et al Konstantin Sokolov Laura M Ricles Seung Yun Nam Function of mesenchymal stem cells following loading of gold nanotracers
description	Laura M Ricles1, Seung Yun Nam1,2, Konstantin Sokolov3,1, Stanislav Y Emelianov1,3, Laura J Suggs11Department of Biomedical Engineering, 2Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, TX, USA; 3Department of Imaging Physics, The University of Texas MD Anderson Cancer Center, Houston, TX, USABackground: Stem cells can differentiate into multiple cell types, and therefore can be used for cellular therapies, including tissue repair. However, the participation of stem cells in tissue repair and neovascularization is not well understood. Therefore, implementing a noninvasive, long-term imaging technique to track stem cells in vivo is needed to obtain a better understanding of the wound healing response. Generally, we are interested in developing an imaging approach to track mesenchymal stem cells (MSCs) in vivo after delivery via a polyethylene glycol modified fibrin matrix (PEGylated fibrin matrix) using MSCs loaded with gold nanoparticles as nanotracers. The objective of the current study was to assess the effects of loading MSCs with gold nanoparticles on cellular function.Methods: In this study, we utilized various gold nanoparticle formulations by varying size and surface coatings and assessed the efficiency of cell labeling using darkfield microscopy. We hypothesized that loading cells with gold nanotracers would not significantly alter cell function due to the inert and biocompatible characteristics of gold. The effect of nanoparticle loading on cell viability and cytotoxicity was analyzed using a LIVE/DEAD stain and an MTT assay. The ability of MSCs to differentiate into adipocytes and osteocytes after nanoparticle loading was also examined. In addition, nanoparticle loading and retention over time was assessed using inductively coupled plasma mass spectrometry (ICP-MS).Conclusion: Our results demonstrate that loading MSCs with gold nanotracers does not alter cell function and, based on the ICP-MS results, long-term imaging and tracking of MSCs is feasible. These findings strengthen the possibility of imaging MSCs in vivo, such as with optical or photoacoustic imaging, to understand better the participation and role of MSCs in neovascularization.Keywords: neovascularization, gold nanoparticles, cell tracking, stem cells, fibrin gel, optical imaging&nbsp;
format	article
author	et al Konstantin Sokolov Laura M Ricles Seung Yun Nam
author_facet	et al Konstantin Sokolov Laura M Ricles Seung Yun Nam
author_sort	et al
title	Function of mesenchymal stem cells following loading of gold nanotracers
title_short	Function of mesenchymal stem cells following loading of gold nanotracers
title_full	Function of mesenchymal stem cells following loading of gold nanotracers
title_fullStr	Function of mesenchymal stem cells following loading of gold nanotracers
title_full_unstemmed	Function of mesenchymal stem cells following loading of gold nanotracers
title_sort	function of mesenchymal stem cells following loading of gold nanotracers
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/35542a2816d54e2f8a9c6793c20bcb8b
work_keys_str_mv	AT etal functionofmesenchymalstemcellsfollowingloadingofgoldnanotracers AT konstantinsokolov functionofmesenchymalstemcellsfollowingloadingofgoldnanotracers AT lauramricles functionofmesenchymalstemcellsfollowingloadingofgoldnanotracers AT seungyunnam functionofmesenchymalstemcellsfollowingloadingofgoldnanotracers
_version_	1718400522763894784

Function of mesenchymal stem cells following loading of gold nanotracers

Ejemplares similares